Activity of ectonucleotidases and adenosine deaminase in rats exposed to cigarette smoke

Thomé, Gustavo R.; Mazzanti, Cinthia M.; Ahmed, Mushtaq; Junior, Marcos Corrêa; Spanevello, Rosélia Maria; Maldonado, Paula; Luchese, Cristiane; Cargnelutti, Denise; Morsch, Vera Maria; Duarte, Marta M. M. F.; Fiorenza, Amanda Maino; Nogueira, Cristina W.; Bona, Karine Santos De; Moretto, Maria Beatriz; Luz, Sônia Cristina Almeida da; Mazzantí, Alexandre; Schetinger, Maria Rosa Chitolina

doi:10.1080/08958370802632267

Cited by 25 publications

(21 citation statements)

References 33 publications

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“…Surprisingly, we found an increase in CD39 expression of inflammatory cells and soluble ATPase activity in the airways of smokers and patients with COPD. Similar observations were also reported in platelets after acute smoke exposure [22,23]. However, in platelets an opposing effect of acute cigarette smoke stimulation on ATP hydrolysing capacity has also been noted [24].…”

Section: Discussionsupporting

confidence: 73%

NTPDase1/CD39 and aberrant purinergic signalling in the pathogenesis of COPD

et al. 2015

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Purinergic receptor activation via extracellular ATP is involved in the pathogenesis of chronic obstructive pulmonary disease (COPD). Nucleoside triphosphate diphosphohydrolase-1/CD39 hydrolyses extracellular ATP and modulates P2 receptor signalling.We aimed to investigate the expression and function of CD39 in the pathogenesis of cigarette smokeinduced lung inflammation in patients and preclinical mouse models. CD39 expression and soluble ATPase activity were quantified in sputum and bronchoalveolar lavage fluid (BALF) cells in nonsmokers, smokers and COPD patients or mice with cigarette smoke-induced lung inflammation. In mice, pulmonary ATP and cytokine concentrations, inflammation and emphysema were analysed in the presence or absence of CD39.Following acute cigarette smoke exposure CD39 was upregulated in BALF cells in smokers with further increases in COPD patients. Acute cigarette smoke exposure induced CD39 upregulation in murine lungs and BALF cells, and ATP degradation was accelerated in airway fluids. CD39 inhibition and deficiency led to augmented lung inflammation; treatment with ATPase during cigarette smoke exposure prevented emphysema.Pulmonary CD39 expression and activity are increased in COPD. CD39 deficiency leads to enhanced emphysema in mice, while external administration of a functional CD39 analogue partially rescues the phenotype. The compensatory upregulation of pulmonary CD39 might serve as a protective mechanism in cigarette smoke-induced lung damage. @ERSpublications The upregulation of pulmonary ATPase is a protective mechanism in cigarette smoke-induced lung inflammation http://ow.ly/S5YcC

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Section: Discussionsupporting

confidence: 73%

NTPDase1/CD39 and aberrant purinergic signalling in the pathogenesis of COPD

et al. 2015

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“…Furthermore, observed an increase in the activity of ADA in the cerebral cortex of rats eight days after neonatal Hypoxia Ischemia [28] and the administration of Metothrexate (MTX) induced a significant decreased in the ADA activity in the cerebral cortex, kidney and liver tissues of young rats [29]. Besides, ADA activity is decreased in plasma of rats exposed to aged and diluted sidestream smoke [30], and in lymphocytes and platelets of patients with multiple sclerosis [31,32].…”

Section: Introductionmentioning

confidence: 95%

<i>Syzygium cumini</i> Extract Decrease Adenosine Deaminase, 5′Nucleotidase Activities and Oxidative Damage in Platelets of Diabetic Patients

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Sari

et al. 2010

Cell Physiol Biochem

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Diabetes mellitus, a chronic metabolic disorder, has assumed epidemic proportions and its long-term complications can have devastating consequences. The oxidative stress in diabetes was greatly increased due to prolonged exposure to hyperglycemia and impairment of oxidant/antioxidant equilibrium. Syzygium cumini is being widely used to treat diabetes by the traditional practitioners over many centuries. Adenosine deaminase (ADA) and 5′-Nucleotidase (5′NT) are enzymes of purine nucleoside metabolism that play an important role in the regulation of adenosine (Ado) levels. In this study, we investigated the effect of Syzygium cumini aqueous leaves extract (ASc) on ADA and 5′NT activities and on parameters of oxidative stress under in vitro conditions, using platelets of patients with Type 2 diabetes mellitus. Platelet-Rich Plasma (PRP) was assayed by ADA, 5′NT, Catalase (CAT), Superoxide Dismutase (SOD) activities and Thiobarbituric acid reactive substances (TBARS) levels. We observed that ADA, 5′NT activities and TBARS levels were significantly higher when compared to the control group, and ASc (100 and 200 µg/mL) prevented these effects. Our study demonstrates that ASc was able to remove oxidant species generated in diabetic conditions and modulates in the Ado levels. Then, ASc may promote a compensatory response in platelet function, improving the susceptibility-induced by the diabetes mellitus.

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“…Control animals were placed in an equal chamber for the same amount of time but without exposure to smoke. When the smoke exposure was carried out, the control group was always outside, without any contact with smoke …”

Section: Methodsmentioning

confidence: 99%

“…A metal grille was placed on top of the cigarette holder to avoid direct contact with the cigarettes and, thus, prevent the rats from injuring themselves. The inhalation exposure of our study was to aged and diluted sidestream smoke, used as a surrogate of environmental tobacco smoke as experienced by non‐smokers …”

Section: Methodsmentioning

confidence: 99%

Effects of curcumin on the activities of the enzymes that hydrolyse adenine nucleotides in platelets from cigarette smoke-exposed rats

et al. 2011

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The aim of the present study was to investigate the effect of curcumin (Cur) on the activity of ectonucleoside triphosphate diphosphohydrolase (CD39), 5'-nucleotidase (CD73) and adenosine deaminase in platelets of cigarette smoke-exposed rats. For that purpose, we subjected male Wistar rats to a treatment with Cur and cigarette smoke, once a day, 5 days each week, for 30 days. The rats were treated by gavage with Cur or corn oil and then exposed to cigarette smoke. The experimental procedures were divided into two sets of experiments. In the first, the animals were divided into four groups: vehicle (corn oil) or Cur 12·5, 25 or 50 mg·kg(-1) . In the second, the animals were divided into five groups: vehicle (corn oil), smoke, or smoke and Cur 12·5, 25 or 50 mg·kg(-1) . The results showed that treatment with Cur significantly prevented the increased adenosine triphosphate (ATP) (121%) and adenosine monophosphate (AMP) (159%) and the decreased adenosine diphosphate (ADP) (51%) hydrolysis observed in the cigarette smoke-exposed rats Our results suggest that those purinergic enzyme alterations observed in the cigarette smoke-exposed rats could be related to an excessive platelet aggregation and point toward the potential of Cur to modulate purinergic signalling and, consequently, regulate the thrombus formation.

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Activity of ectonucleotidases and adenosine deaminase in rats exposed to cigarette smoke

Cited by 25 publications

References 33 publications

NTPDase1/CD39 and aberrant purinergic signalling in the pathogenesis of COPD

NTPDase1/CD39 and aberrant purinergic signalling in the pathogenesis of COPD

<i>Syzygium cumini</i> Extract Decrease Adenosine Deaminase, 5′Nucleotidase Activities and Oxidative Damage in Platelets of Diabetic Patients

Effects of curcumin on the activities of the enzymes that hydrolyse adenine nucleotides in platelets from cigarette smoke-exposed rats

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