Effects of curcumin on the activities of the enzymes that hydrolyse adenine nucleotides in platelets from cigarette smoke-exposed rats

Jaques, Jeandre Augusto dos Santos; Ruchel, Jader Betsch; Schlemmer, Karine Bizzi; Pimentel, Victor Câmera; Bagatini, Margarete Dulce; Souza, Viviane do Carmo Gonçalves; Moretto, Maria Beatriz; Morsch, Vera Maria; Schetinger, Maria Rosa Chitolina; Leal, Daniela Bitencourt Rosa

doi:10.1002/cbf.1798

Cited by 15 publications

(15 citation statements)

References 37 publications

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“…However, as shown in Figure 2C , although the OB-MS group and the RW-MS can be well separated in the OPLS scores plot, CV-ANOVA test of model C in Figure 2 gives poor validity of the model suggesting that larger sample size is required to make the model performance reliable. Like adenosine derivatives, uridine has shown anti-inflammatory effects and appears to affect the tumour necrosis factor (TNF, also known as TNF-alpha) levels in lungs [ 51 ]. Elevated level of uridine and decreased level of unassigned peaks of some pyrimidine metabolites observed in this study suggested that cigarette smoke exposure can cause perturbation of pyrimidine metabolism.…”

Section: Discussionmentioning

confidence: 99%

Metabolite Signatures in Hydrophilic Extracts of Mouse Lungs Exposed to Cigarette Smoke Revealed by 1H NMR Metabolomics Investigation

Wang

Feng

et al. 2015

Metabolomics

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1H-NMR metabolomics was used to investigate the changes of metabolites in the lungs of mice with and without being exposed to a controlled amount of cigarette smoke. It was found that the concentrations of adenosine derivatives (i.e. ATP, ADP and AMP), inosine and uridine were significantly changed in the lungs of mice exposed to cigarette smoke when compared with controls regardless the mice were obese or of regular weight. The decreased ATP, ADP, AMP and elevated inosine suggested that the deaminases in charge of adenosine derivatives to inosine derivatives conversion would be significantly changed in the lungs of mice exposed to cigarette smoke. Indeed, transcriptional study confirmed that the concentrations of adenosine monophosphate deaminase 2 and adenosine deaminase 2 were significantly changed in the lungs of mice exposed to cigarette smoke. We also found that the ratio of glycerophosphocholine (GPC) to phosphocholine (PC) was significantly increased in the lungs of obese mice compared with those of the regular weight mice. The GPC/PC ratio was further elevated in the lungs of obese group exposed to cigarette smoke.

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Section: Discussionmentioning

confidence: 99%

Metabolite Signatures in Hydrophilic Extracts of Mouse Lungs Exposed to Cigarette Smoke Revealed by 1H NMR Metabolomics Investigation

Wang

Feng

et al. 2015

Metabolomics

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“…It is well known that pro‐inflammatory cytokines such as tumour necrosis factor (TNF)–alpha, the interleukin‐1 (IL‐1) family, interleukin‐6 (IL‐6) and auto‐reactive T cells have various important activities in the RA pathogenesis. In this context, the purpose of this study was to investigate the activity of purinergic system ecto‐enzymes, which are important in the regulation of immune and inflammatory responses such as E‐NTPDase and E‐ADA, in lymphocytes isolated from peripheral blood of patients with RA.…”

Section: Discussionmentioning

confidence: 99%

Activities of enzymes that hydrolyze adenine nucleotides in lymphocytes from patients with rheumatoid arthritis

Jaques

Becker

Souza

et al. 2012

Cell Biochemistry & Function

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The purpose of this study was to investigate the activities of ecto-nucleoside triphosphate diphosphohydrolase (E-NTPDase; EC 3.6.1.5; CD39) and adenosine deaminase (E-ADA; EC 3.5.4.4) in lymphocytes from patients with rheumatoid arthritis (RA). Thirty patients diagnosed with RA through American College of Rheumatology criteria as well as 30 healthy patients were selected. Peripheral blood lymphocytes were isolated, and E-NTPDase and E-ADA activities were assayed. The results demonstrated an increased E-NTPDase activity (both ATP and ADP as substrates) and a decreased E-ADA activity in RA patients. These data suggest an organic effort to preserve the adenosine level, which is known to have anti-inflammatory and analgesic properties, working as a potent suppressor of immune response.

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“…We prepared the plasma rich in platelets (PRP) as previously described [34] with modifications [35]. ATP (A6419), ADP (A2754), AMP (A1752), bovine serum albumin (BSA, A2153), trichloroacetic acid (TCA, T4885), 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt (HEPES-H7006) were acquired from Sigma-Aldrich (St. Louis, MO, USA).…”

Section: Assays Of Ectonucleotidase Triphosphate Diphosphohydrolase (mentioning

confidence: 99%

Protective Effect of α-Linolenic Acid on Non-Alcoholic Hepatic Steatosis and Interleukin-6 and -10 in Wistar Rats

et al. 2019

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Consumption of omega-3 (n-3) polyunsaturated fatty acids (PUFA) is related to improvement in the inflammatory response associated with decreases in metabolic disorders of obesity, such as low-grade inflammation and hepatic steatosis. Linseed (Linum usitatissimum) oil is a primary source of n-3 fatty acids (FAs) of plant origin, particularly α-linolenic acid, and provides an alternative for the ingestion of n-3 PUFA by persons allergic to, or wishing to avoid, animal sources. In our study, we evaluated the effect of the consumption of different lipidic sources on metabolic and inflammatory parameters in Wistar rats. We split 56 male rats into four groups that were fed for 60 days with the following diets: sesame oil, (SO, Sesamum indicum), linseed oil (LO), SO + LO (SLO), and a control group (CG) fed with animal fat. Our results reveal that the use of LO or SLO produced improvements in the hepatic tissue, such as lower values of aspartate aminotransferase, liver weight, and hepatic steatosis. LO and SLO reduced the weight of visceral fats, weight gain, and mediated the inflammation through a decrease in interleukin (IL)-6 and increase in IL-10. Though we did not detect any significant differences in the intestine histology and the purinergic system enzymes, the consumption of α-linolenic acid appears to contribute to the inflammatory and hepatic modulation of animals compared with a diet rich in saturated FAs and or unbalanced in n-6/n-3 PUFAs, inferring possible use in treatment of metabolic disorders associated with obesity and cardiovascular diseases.

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Effects of curcumin on the activities of the enzymes that hydrolyse adenine nucleotides in platelets from cigarette smoke-exposed rats

Cited by 15 publications

References 37 publications

Metabolite Signatures in Hydrophilic Extracts of Mouse Lungs Exposed to Cigarette Smoke Revealed by 1H NMR Metabolomics Investigation

Metabolite Signatures in Hydrophilic Extracts of Mouse Lungs Exposed to Cigarette Smoke Revealed by 1H NMR Metabolomics Investigation

Activities of enzymes that hydrolyze adenine nucleotides in lymphocytes from patients with rheumatoid arthritis

Protective Effect of α-Linolenic Acid on Non-Alcoholic Hepatic Steatosis and Interleukin-6 and -10 in Wistar Rats

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