Activity of crude extracts from Brazilian cerrado plants against clinically relevant Candida species

Correia, Amabel Fernandes; Silveira, Dâmaris; Fonseca-Bazzo, Yris Maria; Magalhães, Pérola Oliveira; Fagg, Christopher William; Silva, Elton Clementino da; Gomes, Suelí Maria; Gandolfi, Lenora; Pratesi, Riccardo; Nóbrega, Yanna Karla de Medeiros

doi:10.1186/s12906-016-1164-3

Cited by 34 publications

(25 citation statements)

References 31 publications

(21 reference statements)

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“…However, no assays using Candida species have been carried out. Other species within the genera Eugenia, such as Eugenia dysenterica, have shown antimicrobial activity against several Candida species with MICs ranging between 125 and 500 µg/mL (Correia et al, 2016). These values are similar to those found in the present study using E. uniflora.…”

Section: Discussionsupporting

confidence: 89%

Anti-candida biofilm properties of Cameroonian plant extracts

Elisabeth¹,

Lopez²,

Soto³

et al. 2016

J. Med. Plants Res.

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Candida infections can be superficial, invasive or disseminating. The virulence of Candida species has been attributed to several factors, including the promotion of hyphae and biofilm formation, adherence to host tissues, and response to environmental changes and morphogenesis. Resistance to many clinically used antifungal agents has led to the need to identify new compounds and drugs for therapeutic use. Therefore, the objective of this study was to evaluate the anti-candida and anti-biofilm activities of some Cameroonian plant extracts against Candida albicans and Candida glabrata. The biofilm biomass of C. albicans and C. glabrata was quantified using the violet crystal protocol. A microbroth dilution method was used to determine the minimum inhibitory concentrations (MICs), and a biofilm enumeration assay was employed to determine the minimum biofilm inhibition concentrations (MBICs) and minimum biofilm eradication concentrations (MBECs) of the extracts. The absorbance value of the biofilm biomass of C. albicans was 0.14±0.01 and that of C. glabrata was 0.51±0.06. Eugenia uniflora and Terminalia mantaly aqueous leaf extracts showed MICs of 0.3125 and 0.625 mg/mL for C. glabrata, while the MICs for C. albicans were 10 and 0.625 mg/mL, respectively. The MBIC and MBEC of C. glabrata of E. uniflora aqueous leaf extracts were 0.125 and 0.5 mg/mL, respectively, and 0.45 and >1.8 mg/mL, respectively for T. mantaly. The results of this study demonstrated the in vitro anti-biofilm potential of T. mantaly and E. uniflora aqueous leaf extracts against Candida biofilm. Nonetheless, further analyses of a larger number of Candida isolates and plant extracts are needed to validate these findings.

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Section: Discussionsupporting

confidence: 89%

Anti-candida biofilm properties of Cameroonian plant extracts

Elisabeth¹,

Lopez²,

Soto³

et al. 2016

J. Med. Plants Res.

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“…Additionally, the results observed by Holetz et al [ 27 ], Domingues et al [ 40 ], Paula et al [ 41 ] and Correia et al [ 42 ] showed good activity against yeasts of other species of the Myrtaceae family, suggesting that compounds of M. tomentosa , as avicularin can be used as a potential antifungal.…”

Section: Resultsmentioning

confidence: 95%

Phytochemical Analysis and Antimicrobial Activity of Myrcia tomentosa (Aubl.) DC. Leaves

et al. 2017

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This work describes the isolation and structural elucidation of compounds from the leaves of Myrcia tomentosa (Aubl.) DC. (goiaba-brava) and evaluates the antimicrobial activity of the crude extract, fractions and isolated compounds against bacteria and fungi. Column chromatography was used to fractionate and purify the extract of the M. tomentosa leaves and the chemical structures of the compounds were determined using spectroscopic techniques. The antibacterial and antifungal activities were assessed using the broth microdilution method. The phytochemical investigation isolated 11 compounds: α-bisabolol, α-bisabolol oxide B, α-cadinol, β-sitosterol, n-pentacosane, n-tetracosane, quercetin, kaempferol, avicularin, juglanin and guaijaverin. The crude ethanolic extract and its fractions were tested against 15 bacteria and 9 yeasts. The crude extract inhibited the in vitro growth of yeasts at concentration of 4 to 32 μg/mL. The hexane, dichloromethane, ethyl acetate and aqueous fractions inhibited Candida sp. at concentrations of 4 to 256 μg/mL, whereas the Cryptococcus sp. isolates were inhibited only by the hexane and dichloromethane fractions in minimal inhibitory concentrations (MICs) at 16 to 64 μg/mL. The flavonoid quercetin-3-O-α-arabinofuranose (avicularin) was the most active compound, inhibiting Candida species in concentrations of 2 to 32 μg/mL. The MIC values suggest potential activity of this plant species against yeast.

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“…Several groups have been dedicated to the study of products of natural origin with antifungal action, in order to identify and isolate compounds with effective activity, safety in use and low toxicity against pathogenic fungi (Correia et al, 2016 ).…”

Section: Natural Products With Antifungal Properties Against mentioning

confidence: 99%

“…In relation to plants found mainly in Brazilian territory, Correia et al ( 2016 ) carried out an important study with different plants found in the Brazilian Cerrado, a region with an important number of species used in popular medicine, mainly in studies of essential oils with anti- Candida activity (Nordin et al, 2013 ; Correia et al, 2016 ).…”

Section: Natural Products With Antifungal Properties Against mentioning

confidence: 99%

An Update on Candida tropicalis Based on Basic and Clinical Approaches

2017

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Candida tropicalis has emerged as one of the most important Candida species. It has been widely considered the second most virulent Candida species, only preceded by C. albicans. Besides, this species has been recognized as a very strong biofilm producer, surpassing C. albicans in most of the studies. In addition, it produces a wide range of other virulence factors, including: adhesion to buccal epithelial and endothelial cells; the secretion of lytic enzymes, such as proteinases, phospholipases, and hemolysins, bud-to-hyphae transition (also called morphogenesis) and the phenomenon called phenotypic switching. This is a species very closely related to C. albicans and has been easily identified with both phenotypic and molecular methods. In addition, no cryptic sibling species were yet described in the literature, what is contradictory to some other medically important Candida species. C. tropicalis is a clinically relevant species and may be the second or third etiological agent of candidemia, specifically in Latin American countries and Asia. Antifungal resistance to the azoles, polyenes, and echinocandins has already been described. Apart from all these characteristics, C. tropicalis has been considered an osmotolerant microorganism and this ability to survive to high salt concentration may be important for fungal persistence in saline environments. This physiological characteristic makes this species suitable for use in biotechnology processes. Here we describe an update of C. tropicalis, focusing on all these previously mentioned subjects.

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Activity of crude extracts from Brazilian cerrado plants against clinically relevant Candida species

Cited by 34 publications

References 31 publications

Anti-candida biofilm properties of Cameroonian plant extracts

Anti-candida biofilm properties of Cameroonian plant extracts

Phytochemical Analysis and Antimicrobial Activity of Myrcia tomentosa (Aubl.) DC. Leaves

An Update on Candida tropicalis Based on Basic and Clinical Approaches

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