Activity of ciprofloxacin (BAYo 9867) against pseudomonas aeruginosa and ampicillin-resistant Enterobacteriaceae

C, Roy; A, Foz; Segura, C.; Tirado, M; Teixell, M; Teruel, D. García

doi:10.1007/bf01641358

Cited by 25 publications

(10 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Significantly higher peak levels of ciprofloxacin in serum were observed after multiple dosing (3.51 versus 2.26 ,g/ml; P < 0.01). Increased elimination half-life occurred after multiple dosing; this seems Ciprofloxacin is an investigational quinolone with superior in vitro activity over other DNA gyrase inhibitors (5,17). Single-dose pharmacokinetic and blister fluid penetration studies have been reported after oral and intravenous administration of ciprofloxacin (3, 4, 21), but no studies are available on blister fluid penetration after multiple doses.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Tissue penetration of ciprofloxacin after single and multiple doses

LeBel¹,

Vallée²,

Bergeron³

1986

Antimicrob Agents Chemother

View full text Add to dashboard Cite

The pharmacokinetics and the suction-induced blister fluid penetration of ciprofloxacin were compared after a single dose (500 mg) and after multiple dosing (500 mg q8h for 13 doses). Significantly higher peak levels of ciprofloxacin in serum were observed after multiple dosing (3.51 versus 2.26 ,g/ml; P < 0.01). Increased elimination half-life occurred after multiple dosing; this seems Ciprofloxacin is an investigational quinolone with superior in vitro activity over other DNA gyrase inhibitors (5,17). Single-dose pharmacokinetic and blister fluid penetration studies have been reported after oral and intravenous administration of ciprofloxacin (3, 4, 21), but no studies are available on blister fluid penetration after multiple doses. Multiple-dose pharmacokinetic studies with ciprofloxacin administered twice daily have also been previously conducted (1,8,14).To formulate appropriate dosing schedules for ciprofloxacin, we need to identify the serum and blister fluid concentrations obtained after multiple doses. The purpose of this report was to compare serum and blister fluid concentrations of ciprofloxacin achieved in healthy adult volunteers after administration of a single 500-mg dose and after multiple 500-mg doses given every 8 h over 4 days.(This work was presented in part previously [M. LeBel, F. Vallde, and M. G. Bergeron, 14th Int. Congr. Chemother. 1985, Kyoto, Japan, abstr. no. S-40-2].) MATERIALS AND METHODSSubjects and study desip. Twelve healthy volunteers (six females and six males) between 19 and 25 years old (mean age, 22.3 t 2.0 years) gave their written informed consent to participate in the study. The protocol was approved by the Centre Hospitalier de l'Universite Laval Human Research Review and Pharmacology-Therapeutics committees. The mean weight of the subjects was 62.0 + 7.0 kg (range, 49.5 to 75.0 kg), and the mean body surface area was 1.73 ml (range,

show abstract

mentioning

confidence: 99%

“…Ciprofloxacin is an investigational quinolone with superior in vitro activity over other DNA gyrase inhibitors (5,17). Single-dose pharmacokinetic and blister fluid penetration studies have been reported after oral and intravenous administration of ciprofloxacin (3,4,21), but no studies are available on blister fluid penetration after multiple doses.…”

mentioning

confidence: 99%

Tissue penetration of ciprofloxacin after single and multiple doses

LeBel¹,

Vallée²,

Bergeron³

1986

Antimicrob Agents Chemother

View full text Add to dashboard Cite

show abstract

“…Lower MICs of ciprofloxacin for members of the family Enterobac-teriaceae (including P-lactamase-producing strains) (1, 2, 4-6, 9) and slightly higher MICs of ciprofloxacin for Pseudomonas aeruginosa (including strains resistant to gentamicin or carbenicillin) (2,6,8) were found compared with those of available antimicrobial agents. Other gram-negative and certain gram-positive bacteria were also susceptible to ciprofloxacin.…”

mentioning

confidence: 89%

Quantitation of ciprofloxacin in body fluids by high-pressure liquid chromatography

Weber¹,

Chaffin²,

Smith³

et al. 1985

Antimicrob Agents Chemother

View full text Add to dashboard Cite

We describe a reverse-phase high-pressure liquid chromatography method for the quantitation of a new qumoline carboxylic acid antimicrobial agent, ciprofloxacin (Bay o 9867). This assay utilizes the intrinsic fluorescence of ciprofloxacin for primary detection but employs UV absorption as a secondary detection system. Mobile phases contained methanol and phosphate buffer and used a common C18 ,uBondapak column. A single precipitation step of a 50-,ul specimen was the only sample preparation necessary. The assay is linear from 2,000 to 10 ng/ml and sensitive to 5 ng/ml. The mean recovery of ciprofloxacin from serum was 105.7%. The coefficient of variation was s3.1% for same-day precision and s6.3% for assay-to-assay precision. Because Wise et al. suggest that the mode of antibacterial action of ciprofloxacin is similar to that of nalidixic acid and norfloxacin (9); this suggestion is supported in part by a high correlation between the MICs of ciprofloxacin and norfloxacin (1) (r = 0.96). Ciprofloxacin, however, has demonstrated antimicrobial activity superior to that of naladixic acid and norfloxacin against all pathogens tested (1, 2, 5, 9). A potential use of ciprofloxacin is against systemic infection. This is a departure for this class of antibiotics, which are normally used for the treatment of urinary tract infections (7).Clinical trials and pharmacokinetic studies of ciprofloxacin are needed to study efficacy against pathogens, toxicity (or lack of it), adverse reactions, drug disposition and elimination, and possible emergence of resistance. In two published studies of ciprofloxacin pharmacokinetics (3, 10), samples from a single set of patients were assayed by a plate diffusion method (bioassay). These studies reported 95% confidence limits of ± 19.3 and ± 17.5%, respectively, which were the only indication of accuracy and precision in the assay.Inherently poor precision, antimicrobial factors in patient serum samples, and overnight incubation periods make microbiological bioassays less than ideal. The presence of active antimicrobial metabolites or other antibiotics will also confuse and bias the data.High-pressure liquid chromatography (HPLC) is a rapid, precise, and reliable assay for the quantitation of certain antibiotics. We developed a reverse-phase HPLC method for the quantitation of ciprofloxacin, which uses a common * Corresponding author.C18 ,uBondapak column with two detection systems (UV absorption and fluorescence). This assay takes advantage of the intrinsic fluorescence of ciprofloxacin, enhancing its specificity and sensitivity. This method employs a singlestep precipitation, the only sample preparation necessary, and requires only 50 j,l of specimen. MATERIALS AND METHODSAn HPLC pump (Constrametric I; Laboratory Data Control, Rivera Beach, Fla.) delivered a mobile phase to a previously compressed C18 ,uBondapak radial pack column (8 mm by 10 cm; lots no. P317D01 and P2224D01, Waters Associates, Medford, Mass.) which was under radial compression of ca. 1,600 lb/in2 by an RCM-100 module...

show abstract

“…For instance, both the Providencia genus and enterococci species were fully identified in only one study each (7,12), and the susceptibility of all of the coagulase-negative staphylococcal species has not been previously reported (6,11). Moreover, the activity of ciprofloxacin against strains isolated from Southern Europe has been scarcely evaluated (18).…”

mentioning

confidence: 99%