Activity In Vitro of Clotrimazole against Canine Methicillin-Resistant and Susceptible Staphylococcus pseudintermedius

Abstract

Emergence of multidrug-resistance in Staphylococcus pseudintermedius (SP) has increased interest in topical therapy as an alternative to systemic antibiotics in canine pyoderma. The antifungal imidazole, clotrimazole, is contained in numerous licensed canine ear preparations. Its in vitro activity against SP has not been evaluated, although previous studies have shown that the related imidazole, miconazole, has significant anti-staphylococcal efficacy. We therefore determined minimum inhibitory concentrations (MICs) of clotrimazole amongst 50 SP isolates (25 methicillin-resistant [MR]SP and susceptible [MS]SP) collected from dogs in Germany during 2010–2011 using an agar dilution method (CLSI VET01-A4). MICs amongst MRSP and MSSP were comparable (MIC50 and MIC90 = 1mg/L for both groups, p = 0.317); overall, 49 isolates had MIC = 1 mg/L and one had MIC = 0.5 mg/L. The relatively low MICs obtained in this study are likely to be exceeded by topical therapy and thus further clinical evaluation of clotrimazole use in canine superficial pyoderma and otitis externa caused by MRSP and MSSP is now warranted.

Antibiotic Susceptibility Profile of Pseudomonas aeruginosa Canine Isolates from a Multicentric Study in Romania

“…Pseudomonas isolates in canine pyodermatitis have been confirmed in numerous other studies carried out in several geographic regions [19,20]. In agreement with our findings regarding the presence of multidrug-resistant Pseudomonas in canine pyodermatitis lesions, multiple data are reported in previous surveys conducted by Wildemuth et al [21], based on comparing the susceptibility of Pseudomonas spp.…”

Antibiotic Susceptibility Profile of Pseudomonas Aeruginosa Canine Isolates from a Multicentric Study in Romania

“…The involvement of Pseudomonas isolates in canine pyodermatitis has been confirmed in numerous other studies carried out in several geographic regions [21,22].…”

Clinically used antifungal azoles as ligands for gold(iii) complexes: the influence of the Au(iii) ion on the antimicrobial activity of the complex

“…Interestingly, S. aureus MRSA was the most sensitive, with MIC of 14.1 and 32.2 µM (5.4 and 12.5 µg mL −1 ) for ecz and tcz, respectively. Previously, ctz, ecz and tcz were shown to affect the growth of Staphylococcus strains [53][54][55][56] and some Gramnegative bacterial species, however there are no available data on the antibacterial activity of their metal complexes. Complexation of the investigated azoles with Au(III) ion resulted in the emergence of the activity against other bacterial strains such as E. coli, with the lowest MIC of 30.9 µM (20 µg mL −1 ) for complex 4.…”