Antibiotic Susceptibility Profile of Pseudomonas aeruginosa Canine Isolates from a Multicentric Study in Romania

Abstract

Treating infections caused by Pseudomonas aeruginosa is increasingly difficult due to high antibiotic resistance, materialized through the presence of multiple resistance strains, as well as due to rapid development of resistance throughout treatment. The present survey was conducted to investigate the antibiotic susceptibility profile of Pseudomonas aeruginosa pathogens in two University Veterinary hospitals from different geographical regions of Romania (i.e., Southwest Timișoara county and Northeast Iași county) involved in superficial canine infections. A total of 142 swab specimens were collected from dogs with superficial infections (superficial skin infections, otitis externa, and perianal abscess) to assess the presence of Pseudomonas aeruginosa, based on phenotypic and molecular characterization. According to their confirmed morphological and molecular features, 58 samples (40.84%; 58/142) were positive for Pseudomonas aeruginosa (according to their confirmed morphological and molecular features). Antibiotic susceptibility testing for 12 antibiotics was conducted using the Kirby–Bauer disc diffusion method. Drug resistance was observed in the case of all tested antibiotics. The susceptibility rate of P. aeruginosa strains that were tested in this study was in the following order: ceftazidime (53.44%; 31/58), followed by aztreonam (51.72%; 30/58), amikacin (44.82%; 26/58), azithromycin (41.37%; 24/58), gentamicin (37.93%; 22/58), cefepime (36.20%; 21/58), meropenem (25.86%; 13/58), piperacillin-tazobactam (25.86%; 13/58), imipenem (22.41%; 13/158), ciprofloxacin (17.24%; 10/58), tobramycin (8.62; 5/58), and polymyxin B (1.72; 1/58). The results highlight the importance of antibiotic susceptibility testing in Pseudomonas aeruginosa isolates from dogs with superficial infections to use an adequate treatment plan to manage the skin condition and other pathologies (otitis externa and perianal abscesses).

Genetic Diversity, Biofilm Formation, and Antibiotic Resistance of Pseudomonas aeruginosa Isolated from Cow, Camel, and Mare with Clinical Endometritis

“…P. aeruginosa possesses a naturally-occurring AmpC β-lactamase that is not inhibited by the currently available lactam inhibitors, clavulanic acid, sulbactam, and tazobactam, and therefore confers resistance to antibiotic combinations [ 87 , 88 ]. Several earlier studies have reported high proportions of P. aeruginosa resistance to ceftazidime and aztreonam [ 84 , 89 , 90 , 91 ]. This is consistent with the 39% resistance observed in this study.…”

Clonal Dissemination of Extended-Spectrum Cephalosporin-Resistant Enterobacterales between Dogs and Humans in Households and Animal Shelters of Romania

“…Despite this concerning record, there is limited information on the prevalence of AMR in bacterial populations from humans [ 18 , 19 , 20 , 21 ] and, with the exception of a few studies on the prevalence of Staphylococcus spp. in dogs [ 22 , 23 , 24 ], AMR data in companion animals is lacking. In this study, we found that 44.11% of healthy dogs from households carried ESC-R Enterobacterales , which is similar to carriage rates of cefotaxime-resistant E. coli isolates found in healthy dogs in the Netherlands (45%) [ 14 ], and slightly lower than that reported in dogs from South Korea (49%) [ 25 ].…”

Antibiotic resistance profiles and activity of clove essential oil (Syzygium aromaticum) against Pseudomonas aeruginosa isolated of canine otitis

“…The low susceptibility of the isolates to anti-pseudomonal antibiotics, such as aztreonam, imipenem, ceftazidime, cefepime, and piperacillin associated with tazobactam, is alarming. It reflects the skillful defense developed by P. aeruginosa against these agents and the scarce remaining therapeutic resources [ 36 , 37 ]. Another valuable finding was the greater susceptibility of the isolates to ceftazidime ( Table-1 ) than to cefepime, as the latter is a fourth-generation cephalosporin.…”