Activity-Directed Fractionation ofPleurotus ostreatus. in the Search for Analgesics

Abstract

Pleurotus ostreatus (Jacquin: Fries) P. Kummer (Tricholomataceae) was subjected to an antinociceptive activityguided fractionation procedure in order to identify the fraction having highest activity. Three extracts, namely, acetone, dichloromethane, and hexane, were prepared at room temperature using fresh P. ostreatus, and 500 mg/kg was orally administered to male rats. The acetone extract showed significant antinociceptive activity with the hot-plate assay (male, 68%; p < 0.05), whereas dichloromethane and hexane extracts did not show significant activity. The same dose of acetone extract with female rats in the diestrous stage also showed prolongation of reaction time using the hot-plate test (female, 54%; p < 0.05). None of the extracts showed a significant increase in reaction time with the tail-flick test. The acetone extract was further fractionated by solvent partition to obtain four fractions (hexane, dichloromethane, ethyl acetate, and aqueous). Of these extracts, only the aqueous fraction showed marked prolongation in reaction time with the hot-plate test (500 mg/kg dose) on both male rats and on female rats in the diestrous stage (male, 37%; female, 26%). The aqueous fraction, following purification on a reverse phase column, yielded AqFrA-1, AqFrA-2, and AqFrA-3. Oral administration of a 500 mg/kg dose of these three fractions also showed marked prolongation in reaction time with the hot-plate test after 1 h of treatment (AqFrA-1, 26%; AqFrA-2, 69%; AqFrA-3, 101%). The effect was highest in AqFrA-3 at 1 h of treatment, and the effect lasted for 3 h. Hence, we can conclude that the compounds responsible for the activity have very high polarity. The acetone extract of P. ostreatus did not induce a membrane-stabilizing effect and did not cause prostaglandin inhibition. Naloxone blocked the antinociceptive activity in the hot-plate test upon feeding of 500 mg/kg of acetone extract of P. ostreatus, Accepted: October 8, 2007.Address correspondence to: D.T.U. Abeytunga, Department of Chemistry, University of Colombo, Colombo 3, Sri Lanka. E-mail: thusitha@chem.cmb.ac.lk indicating the mechanism of action is via opioid receptor mediation.

Yield, size, nutritional value, and antioxidant activity of oyster mushrooms grown on perilla stalks

“…Its extract can lower cholesterol as effectively as dietary supplements (Khatun et al, 2007). Furthermore, P. ostreatus has potent antinociceptive, antitumor, antioxidant, and immunological activities (Jayakumar et al, 2009, Vasudewa et al, 2008, Sarangi et al, 2006). P. ostreatus has been cultivated using agroindustrial residues from banana (Reddy et al, 2003), olive (Ruiz-Rodriguez et al, 2010, Kalmis et al, 2008), and weed plants ( Khatun et al, 2007), as well as crop waste, including soybean, cotton, and wheat stalk and sugar cane remnants (Khatun et al, 2007).…”

Anti‐Inflammatory Activity of Pleurotus ostreatus, a Culinary Medicinal Mushroom, in Wistar Rats

“…Planas et al [42] evaluated the opioid receptor-mediated activities on suppression of the inflammation in the carrageenan-induced paw oedema. e SFDP P.o and AE of P.o have been shown to act via opioid mechanisms in inducing antinociception action in rats [9,43]. erefore, P.o may also act via opioid receptors to induce anti-inflammatory activity.…”

Antioxidant activity and cytotoxicity of the edible mushroom, Pleurotus cystidiosus against Hep-2 carcinoma cells

“…Extraction and fractionation of P. cystidiosus mushroom was carried out using the procedure of Vasudewa et al (2008). This procedure was very successful, giving a fraction with potent anti nociceptive activity.…”