Activity and Stability Studies of Verbascoside, a Novel Antioxidant, in Dermo-Cosmetic and Pharmaceutical Topical Formulations

Abstract

We here report the results of our investigations carried out on verbascoside, a phenylpropanoid glycoside known for its antioxidant, anti-inflammatory and photoprotective actions. Verbascoside was obtained from Buddleia davidii meristematic cells, obtained in turn using a sustainable biotechnology platform which employs an in vitro plant cell culture technology. Verbascoside was first investigated to assess the behaviour of the active ingredient in solution or in finished preparations, in view of its potential topical use, especially in skin protection. Stability studies were performed by HPLC, and a PCL assay was adopted to determine the radical scavenging activity toward superoxide anion. The high hydrophilic character of verbascoside, suggested in a somewhat limited range of possible applications, leading us to explore its derivatization to obtain the semi-synthetic derivative VPP, an acyl derivative of verbascoside, with an improved range of applications due to its lower hydrophilic profile. Alone, VPP revealed increased antioxidant activity, both as an active ingredient and in dermocosmetic preparations. Stability studies showed a greater stability of VPP in lipophilic vehicles, whereas the parent verbascoside proved more stable in an O/W emulsions. Verbascoside was also stable in suppositories, an interesting pharmaceutical form for possible applications in treatment of inflammation of the intestinal mucosa.

Filamentous fungi from Plantago lanceolata L. leaves: Contribution to the pattern and stability of bioactive metabolites

“…IGs were stable in controls during the incubation period (no significant degradation detected, p>0.05), but acteoside showed an exponential decay curve, 31.5 % of the initial concentration remained by the 15th day. The compound is considered somewhat unstable in solution, as described by (Vertuani et al, 2011). .…”

Evaluation of anticandidal and antioxidant activities of phenolic compounds from Pyrostegia venusta (Ker Gawl.) Miers

“…This phenylpropanoid glycoside has numerous therapeutic effects; its antioxidant [31,36,37], kinase C inhibitor [38], immunomodulatory [39,40], hepatoprotective [41], anti-inflammatory [42][43][44], analgesic [45], and anti-asthmatic [46] actions have already been validated. Verbascoside has also been indicated to treat patients with the Parkinson disease, an oxidative stress-induced neurodegenerative condition [47].…”

“…Innovative herbal medicines containing verbascoside may become a reality, especially if we consider that this substance remains stable in pharmaceutical formulations and dermocosmetics [31]. Besides P. venusta, there have been reports that the dry biomass of roseroot species yields approximately 16% of this biologically active component [32].…”

Methods for the Evaluation of the Protective Efficacy of Sunscreen Products

“…As a defense system against the action of free radicals in this optical window, topical antioxidants incorporated in cosmetic formulations [18,19,20], and the systemic [21,22,23] and combined application of antioxidants [21,24,25], are advised. Additionally, antioxidants protect cosmetic formulations [26,27] against oxidation, thus extending their lifetime.…”