Activities of chalcone synthase and UDPGal: flavonoid-3-o-glycosyltransferase in relation to anthocyanin synthesis in apple

Ju, Zhiguo; Liu, Chenglian; Yuan, Yahong

doi:10.1016/0304-4238(95)00807-6

Cited by 90 publications

(62 citation statements)

References 27 publications

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“…These genes were expressed in a highly correlative way with MrMYB1 expression, as cultivar, tissue and light regulation were consistent with a primary effect on MrMYB1, which then affects the pathways expression. CHS and CHI were least influenced by tissue, cultivar or fruit bagging treatments, suggesting its non-rate limiting role in anthocyanin biosynthesis, as has been observed in some other plant species (Ju et al 1995;Boss et al 1996;Lo Piero et al 2005;Ahmed et al 2009;Yuan et al 2009). The EBG have been separated by such behavior from LBG in species such as grape, wheat and gentian (Boss et al 1996;Kobayashi et al 2002;Nakatsuka et al 2008;Ahmed et al 2009).…”

Section: The Bayberry Anthocyanin Pathwaymentioning

confidence: 77%

Coordinated regulation of anthocyanin biosynthesis in Chinese bayberry (Myrica rubra) fruit by a R2R3 MYB transcription factor

Niu

Zhang

et al. 2010

Planta

248

166

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Chinese bayberry (Myrica rubra) is a fruit crop with cultivars producing fruit ranging from white (Shuijing, SJ) to red (Dongkui, DK) and dark red-purple (Biqi, BQ), as a result of different levels of anthocyanin accumulation. Genes encoding the anthocyanin biosynthesis enzymes chalcone synthase, chalcone isomerase, flavanone 3-hydroxylase (F3H), flavonoid 3'-hydroxylase (F3'H), dihydroflavonol 4-reductase (DFR), anthocyanidin synthase (ANS) and UDPglucose: flavonoid 3-O-glucosyltransferase (UFGT), as well as MrMYB1, a R2R3 MYB transcription factor homologous to known activators of anthocyanin biosynthesis, were isolated from ripe fruit of BQ. Differences in mRNA abundance of MrF3H, MrF3'H, MrDFR1, MrANS and MrUFGT were highly correlated with differential accumulation of anthocyanins between cultivars, suggesting coordinated regulation by transcription factors. The transcript level of MrMYB1 was strongly associated with the anthocyanin content in ripe fruit of the three cultivars, as well as different anthocyanin containing tissues of BQ fruit. Fruit bagging strongly inhibited anthocyanin accumulation in fruit as well as the expression of all anthocyanin biosynthetic genes and MrMYB1. Overexpression of MrMYB1 stimulated both anthocyanin accumulation and activated an Arabidopsis-DFR promoter in tobacco (Nicotiana tabacum). MrMYB1d, an allele with a 1 bp deletion at nucleotide 30 of coding sequence, was observed in SJ and DK fruit, suggesting that a nonsense mutation of the MYB1 protein may be responsible for no or low expression of MYB1 in the white and red fruit. These results show that coordinated expression of multiple biosynthetic genes is involved in anthocyanin accumulation in Chinese bayberry fruit, and this is regulated by MrMYB1.

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Section: The Bayberry Anthocyanin Pathwaymentioning

confidence: 77%

Coordinated regulation of anthocyanin biosynthesis in Chinese bayberry (Myrica rubra) fruit by a R2R3 MYB transcription factor

Niu

Zhang

et al. 2010

Planta

248

166

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“…For example, quercetin glycosides, such as rutin (quercetin 3-rutinoside), have a cyanidin glycosides-like structure and are among the main flavonoids in citrus leaves (Kawaii et al 2000). UFGTs from red cabbage seedlings and apple are able to use quercetin more efficiently than cyanidin as a substrate (Sun and Hrazdina 1991;Ju et al 1995). Recently, Offen et al (2006) showed that red grape UFGT (VvGT1), responsible for the formation of anthocyanins, is active, in vitro, on a range of flavonoids.…”

Section: Discussionmentioning

confidence: 98%

Cloning and molecular characterization of R2R3-MYB and bHLH-MYC transcription factors from Citrus sinensis

Cultrone

Cotroneo

Recupero

2009

Tree Genetics & Genomes

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Members of the MYB and MYC family regulate the biosynthesis of phenylpropanoids in several plant species. Two sequences, called CsMYB8 and CsMYC2, were identified from Citrus sinensis, and both the cDNA and the genomic clones were isolated and characterized from the flesh of common and blood oranges. Analysis by real-time polymerase chain reaction showed that the expression pattern of CsMYC2 is generally higher in rind than in flesh and in blood oranges than in common ones. In contrast, no significant difference in expression was observed for CsMYB8. The expression pattern of the structural genes chalcone synthase, anthocyanidin synthase, and UDP-glucose-flavonoid 3-O-glucosyltransferase, which code for three enzymes involved in the anthocyanin biosynthetic pathway, was also analyzed and correlated with CsMYC2, in flesh, rind, and leaf of the common and blood oranges, and in leaf of Citrus limon cultivars (characterized by anthocyanin absence or variable content). Surprisingly, CsMYC2 is highly expressed in the leaf and expression is correlated with UFGT expression in this organ. These results suggest that CsMYC2 is involved in the regulation of the flavonoid biosynthetic pathway in Citrus.

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“…CHS and UFGT activities were assayed as described by Ju et al (1995) and Ghasemzadeh et al (2012), respectively. Briefly, the reaction mixture for UFGT activity contained 100 mL of enzyme extract, 1 mM UDP-Glc dissolved in 20 mL of a 330 mM solution of quercetin in methanol, and additional extraction buffer to produce a 250-mL total volume.…”

Section: Enzymatic Assaysmentioning

confidence: 99%

Developmental and metabolic plasticity of white-skinned grape berries in response to Botrytis cinerea during noble rot

et al. 2015

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Noble rot results from exceptional infections of ripe grape (Vitis vinifera) berries by Botrytis cinerea. Unlike bunch rot, noble rot promotes favorable changes in grape berries and the accumulation of secondary metabolites that enhance wine grape composition. Noble rot-infected berries of cv Sémillon, a white-skinned variety, were collected over 3 years from a commercial vineyard at the same time that fruit were harvested for botrytized wine production. Using an integrated transcriptomics and metabolomics approach, we demonstrate that noble rot alters the metabolism of cv Sémillon berries by inducing biotic and abiotic stress responses as well as ripening processes. During noble rot, B. cinerea induced the expression of key regulators of ripening-associated pathways, some of which are distinctive to the normal ripening of red-skinned cultivars. Enhancement of phenylpropanoid metabolism, characterized by a restricted flux in white-skinned berries, was a common outcome of noble rot and red-skinned berry ripening. Transcript and metabolite analyses together with enzymatic assays determined that the biosynthesis of anthocyanins is a consistent hallmark of noble rot in cv Sémillon berries. The biosynthesis of terpenes and fatty acid aroma precursors also increased during noble rot. We finally characterized the impact of noble rot in botrytized wines. Altogether, the results of this work demonstrated that noble rot causes a major reprogramming of berry development and metabolism. This desirable interaction between a fruit and a fungus stimulates pathways otherwise inactive in white-skinned berries, leading to a greater accumulation of compounds involved in the unique flavor and aroma of botrytized wines.

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Activities of chalcone synthase and UDPGal: flavonoid-3-o-glycosyltransferase in relation to anthocyanin synthesis in apple

Cited by 90 publications

References 27 publications

Coordinated regulation of anthocyanin biosynthesis in Chinese bayberry (Myrica rubra) fruit by a R2R3 MYB transcription factor

Coordinated regulation of anthocyanin biosynthesis in Chinese bayberry (Myrica rubra) fruit by a R2R3 MYB transcription factor

Cloning and molecular characterization of R2R3-MYB and bHLH-MYC transcription factors from Citrus sinensis

Developmental and metabolic plasticity of white-skinned grape berries in response to Botrytis cinerea during noble rot

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