Activities of alkaline phosphatase, glutamate oxaloacetate transaminase and glutamate pyruvate transaminase in liver and serum of <I>Trypanosoma brucei</I>-infected rats treated with honey

Abstract

Alkaline phosphatase, glutamate oxaloacetate transaminase and glutamate pyruvate transaminase activities were assessed in rats highly infected with federe strain of Trypanosoma brucei and treated with honey. Therapeutic effect of honey on parasitaemia was also assessed. Results show an extension in the life span of infected but treated rats from 12 days for control to 19 days for infected honey-treated rats. Parasitaemia was also effectively lowered. Alkaline phosphatase activity was increased in the serum but not significantly different in the liver. Glutamate oxaloacetate transaminase and glutamate pyruvate transaminase were also increased in the serum but there is decrease in glutamate pyruvate transaminase activity in the liver. There were no significant differences in glutamate oxaloacetate transaminase activity in the liver. Results suggest that, honey could be a potential agent in the management of African trypanosomosis. However, it leads to decrease in glutamate pyruvate transaminase activity in the liver suggesting liver damage. Increase in glutamate oxaloacetate transaminase and alkaline phosphatase activities in the serum also suggest possible damage to other organs where these maybe abundant.

Some biochemical and haematological effects of black seed (Nigella sativa) oil on T. brucei-infected rats

“…This suggests and probably confirms earlier results (Ekanem and Yusuf, 2005;Ekanem et al, 2006) that infection could lead to gradual tissue especially liver destruction as decreases in the liver enzyme activities are also observed. Treatment with black seed oil however enhanced the situation as observed in the further increases in the activities of alkaline phosphatase and Glutamate oxaloacetate transaminase (Tables 2 and 3).…”

“…The suspension as described earlier (Ekanem and Yusuf, 2005;Ekanem et al, 2006) contained 3 or 4 trypanosomes per view at x100 magnification (approximately 10 6 cells per ml).…”

“…Serum and liver collection as well as alkaline phosphatase, glutamate pyruvate transaminase and glutamate oxaloacetate transaminase assays were carried out as earlier described (Ekanem and Yusuf, 2005;Ekanem et al, 2006).…”

“…Formulations or natural products which boost the host immune system and possibly reduce parasitaemia or completely remove parasites from the host system could contribute extensively to the control or management of the disease (Hoet et al, 2004;Chibale, 2005). We have earlier reported that the administration of honey to Trypanosoma brucei-infected rats was able to reduce the parasitaemia and extensively extend the life span of infected rats when compared with infected nontreated rats (Ekanem and Yusuf, 2005), even when included as part of the diet (Ekanem et al, 2006). Honey is a natural product reported to have immune-boosting properties (Abuharfeil, 1999).…”

Biochemical and morphological changes in Trypanosoma brucei brucei- infected rats treated with homidium chloride and diminazene aceturate

“…(1) . It is a scourge for which both humans and animals stand the risk of epidemics (2) and could be fatal if left untreated. The control of trypanosomosis over the years has largely relied on two broad strategies including the use of chemotherapeutic agents and vector control (3,4) .…”