Actividad antimicrobiana de hongos endofíticos aislados de Brugmansia suaveolens Bercht. & J. Presl

Abstract

Natural products produced from endophyte fungi have a broad spectrum of biological activity and can be grouped into several categories. Antimicrobials constitute an important group of therapeutic agents, which can be produced and obtained from living organisms. The main objective of this study is to investigate the endophytic fungi and the leaves of Brugmansia suaveolens Bercht. & J. Presl as elicitors, aiming at the production of bioactive substances with antimicrobial properties. The extracts were prepared from 19 fungi isolated from the leaves of B. suaveolens Bercht. & J. Presl, used as a biotic elicitor. The extracts were obtained by fermentation in a submerged medium with the addition of leaves from its dry leaves, and the antimicrobial activity of its extracts was evaluated using the agar diffusion method. The results showed that 57% of the endophytic fungi showed antimicrobial activity after fermentation with the elicitor. It was observed that the antimicrobial activity was increased compared to control cultures. The leaves of B. suaveolens Bercht. & J. Presl presented bioactives that stimulated the production of a substance with antimicrobial activity by endophytic fungi, the study of such compounds showed promise to clarify the application potential of these bioactives.

Therapeutic Potential of Bioactive Compounds from Brugmansia suaveolens Bercht. & J. Presl

“…It grows in Peru, Bolivia, and Ecuador in the Solanaceae family, genus Brugmansia, and species suaveolens; its growth is in the form of shrubs, reaching approximately 3–9 m in height, or even more in favorable conditions. It has white or pink flowers but presents color variations; it is aromatic, in the shape of a trombette, and can measure up to 15–50 cm, from where its popular name arises, “Trompete”, as well as other popular names such as “Saia Branca”, “Cartuchiller”, “Canudo”, or “Zabumba” [ 3 , 4 ].…”

“…Brugmansia suaveolens Bercht. & J. Presl present different secondary metabolites, which include alkaloids, steroids, phenolic compounds, terpenes, triterpenes, and flavonoids, among others, related to therapeutic potential [ 4 , 60 , 61 ]. The main metabolites are the tropane alkaloids related to significant biological activities and recognized as the main secondary metabolites in medications derived from plants [ 62 ].…”

“…It is considered the third most economically important plant family and the first among vegetables. Some examples of these species are tomato ( Solanum lycopersicum ), with major importance in agriculture, and, for the pharmaceutical industry, Atropa belladonna stands out, among others [ 3 , 4 ].…”

“…& J. Presl), widely cultivated as an ornamental piece due to its characteristic odor and the beauty of its flowers. Within the research scope, plants belonging to the Solanaceae family are known to produce tropane alkaloids, a group of commonly toxic secondary metabolites used as plant defense [ 4 , 5 ].…”

“…and J. Presl is still a little-reported species regarding its biological effects, which enable the elucidation of substances of natural origin, stimulating the discovery of new products with different applications. This lack of comprehensive reports enables unprecedented studies using substances of natural origin, stimulating the discovery of new products with different applications [ 4 , 8 , 12 ].…”