Active RNA interference in mitochondria

Gao, Kuanxing; Cheng, Man; Zuo, Xinxin; Lin, Jinzhong; Hoogewijs, Kurt; Murphy, Michael P.; Fu, Xiang-Dong; Zhang, Xiaorong

doi:10.1038/s41422-020-00394-5

Cited by 36 publications

(33 citation statements)

References 51 publications

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“…A recent study reported an active argonaute 2-dependent RNAi mechanism in controlling targeted mRNA levels in animal and human mitochondria (mitoRNAi). 64 However, there is no evidence of the co-occurrence of nuclear-encoded RNA-induced silencing complex (RISC) components in plant chloroplasts. 62 Interestingly, overexpression of long dsRNA molecules in plastomes partially produced unexpected lower molecular weight transcripts than the desired full-length double-stranded structures, suggesting the existence of an active RNA processing mechanism in plastids.…”

Section: Resultsmentioning

confidence: 99%

Non-transgenic Gene Modulation via Spray Delivery of Nucleic Acid/Peptide Complexes into Plant Nuclei and Chloroplasts

et al. 2022

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Genetic engineering of economically important traits in plants is an effective way to improve global welfare. However, introducing foreign DNA molecules into plant genomes to create genetically engineered plants not only requires a lengthy testing period and high developmental costs but also is not well-accepted by the public due to safety concerns about its effects on human and animal health and the environment. Here, we present a high-throughput nucleic acids delivery platform for plants using peptide nanocarriers applied to the leaf surface by spraying. The translocation of sub-micrometer-scale nucleic acid/peptide complexes upon spraying varied depending on the physicochemical characteristics of the peptides and was controlled by a stomata-dependent-uptake mechanism in plant cells. We observed efficient delivery of DNA molecules into plants using cell-penetrating peptide (CPP)-based foliar spraying. Moreover, using foliar spraying, we successfully performed gene silencing by introducing small interfering RNA molecules in plant nuclei via siRNA-CPP complexes and, more importantly, in chloroplasts via our CPP/chloroplast-targeting peptide-mediated delivery system. This technology enables effective nontransgenic engineering of economically important plant traits in agricultural systems.

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Section: Resultsmentioning

confidence: 99%

Non-transgenic Gene Modulation via Spray Delivery of Nucleic Acid/Peptide Complexes into Plant Nuclei and Chloroplasts

et al. 2022

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“…Moreover, we could not apply the classical mitochondria-targeted coding sequence, such as Su9, 71 owing to the noncoding character of lncRNAs. It was recently revealed that an RNA sequence longer than 77 nt could not be imported into the mitochondria, 72 thereby indicating the limitation of the current technology.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of cytosolic long noncoding RNA cytb protects against pressure-overload-induced heart failure via sponging microRNA-103-3p

Zhang

Yuan

Liu

et al. 2022

Molecular Therapy - Nucleic Acids

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“…If so, this would be an important mechanism of gene-specific regulation. However, the mitochondrial localization and function of RNAi machinery elements have not been studied systematically, with the exception of a report that shows a surprising ability to transfect mitochondria [ 149 ]. In summary, further study on the interesting concept of RNAi-mediated regulation of human mitochondrial gene expression is required.…”

Section: Unresolved Issuesmentioning

confidence: 99%

Human Mitochondrial RNA Processing and Modifications: Overview

Jedynak-Slyvka

Jabczynska

Szczęsny

2021

IJMS

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Mitochondria, often referred to as the powerhouses of cells, are vital organelles that are present in almost all eukaryotic organisms, including humans. They are the key energy suppliers as the site of adenosine triphosphate production, and are involved in apoptosis, calcium homeostasis, and regulation of the innate immune response. Abnormalities occurring in mitochondria, such as mitochondrial DNA (mtDNA) mutations and disturbances at any stage of mitochondrial RNA (mtRNA) processing and translation, usually lead to severe mitochondrial diseases. A fundamental line of investigation is to understand the processes that occur in these organelles and their physiological consequences. Despite substantial progress that has been made in the field of mtRNA processing and its regulation, many unknowns and controversies remain. The present review discusses the current state of knowledge of RNA processing in human mitochondria and sheds some light on the unresolved issues.

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Active RNA interference in mitochondria

Cited by 36 publications

References 51 publications

Non-transgenic Gene Modulation via Spray Delivery of Nucleic Acid/Peptide Complexes into Plant Nuclei and Chloroplasts

Non-transgenic Gene Modulation via Spray Delivery of Nucleic Acid/Peptide Complexes into Plant Nuclei and Chloroplasts

Overexpression of cytosolic long noncoding RNA cytb protects against pressure-overload-induced heart failure via sponging microRNA-103-3p

Human Mitochondrial RNA Processing and Modifications: Overview

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