Overexpression of cytosolic long noncoding RNA cytb protects against pressure-overload-induced heart failure via sponging microRNA-103-3p

Zhang, Xudong; Yuan, Shuai; Liu, Jingbo; Tang, Yuyan; Wang, Yan; Zhan, Jiabing; Fan, Jiahui; Nie, Xiang; Zhang, Yanru; Wen, Zheng; Li, Huaping; Chen, Chen; Wang, Dao Wen

doi:10.1016/j.omtn.2022.02.002

Cited by 11 publications

(7 citation statements)

References 84 publications

(97 reference statements)

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“…Previous research has shown that miR-103-3p is negatively linked to cardiomyocyte fate ( Dhingra et al, 2015 ; Wang et al, 2015 ). Zhang et al indicated that overexpression of cytosolic long noncoding RNA cytb protects against pressure-overload-induced heart failure by sponging microRNA-103-3p ( Zhang et al, 2022 ). In our study, we found that the mmu-Ryr2_0040---miR-103-3p---Celsr2/Prmt8 axis may be a novel target in cardiac hypertrophy.…”

Section: Discussionmentioning

confidence: 99%

Identification of circular RNAs in cardiac hypertrophy and cardiac fibrosis

et al. 2022

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Cardiac hypertrophy initially serves as an adaptive response to physiological and pathological stimuli. Sustained hypertrophy progress to pathological cardiac hypertrophy, cardiac fibrosis and ultimately lead to heart failure, one of the leading medical causes of mortality worldwide. Intervention of pathological cardiac hypertrophy can effectively reduce the occurrence of heart failure. Abundant factors, such as adrenergic, angiotensin, and endothelin (ET-1) receptors, have been shown to participate in the regulation of pathological cardiac hypertrophy. Recently, an increasing number of studies have indicated that circRNA and circRNA-miRNA–mRNA network regulation is indispensable for the posttranscriptional regulation of mRNA in cardiac hypertrophy. In our study, the morphological, cardiac function and pathological changes during cardiac hypertrophy were investigated. RNA sequencing identified 93 circRNAs that were differentially expressed in the TAC_2w group, and 55 circRNAs in the TAC_4w group compared with the sham group. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses identified several significant pathways, including hypertrophic cardiomyopathy, extracellular matrix (ECM)-receptor interaction and focal adhesion. Coexpression analyses were performed for differentially expressed circRNAs and differentially expressed mRNAs. Based on gene set enrichment analysis (GSEA), 8 circRNAs (mmu-Nfkb1_0001, mmu-Smad4_0007, mmu-Hecw2_0009, mmu-Itgbl1_0002, mmu-Lrrc2_0005, mmu-Cpeb3_0007, mmu-Ryr2_0040, and mmu-Rtn4_0001) involved in cardiac hypertrophy and cardiac fibrosis were identified. We validated some key circRNAs by qPCR. The crucial coexpression of circRNA–mRNA and its interaction with miRNA showed the possible mechanism of circRNAs in the process of cardiac dysfunction. Our results may provide promising targets for the treatment of pathological cardiac hypertrophy and fibrosis.

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Section: Discussionmentioning

confidence: 99%

Identification of circular RNAs in cardiac hypertrophy and cardiac fibrosis

et al. 2022

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“…β-Actin and U6 were used as internal references for mRNA and miRNA transcript-levels quantification, respectively. Absolute qRT-PCR was performed as reported previously ( 35 ). Standard curves of mir222hg and miR146a-5p were generated via the serial dilution of standard mir222hg (Tsingke Biotechnology, Beijing, China) and standard miR146a-5p (Accurate Biotechnology, Hunan, China).…”

Section: Methodsmentioning

confidence: 99%

MIR222HG attenuates macrophage M2 polarization and allergic inflammation in allergic rhinitis by targeting the miR146a-5p/TRAF6/NF-κB axis

Wen

Tang

et al. 2023

Front. Immunol.

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Although M2 macrophages are involved in the orchestration of type 2 inflammation in allergic diseases, the mechanisms underlying non-coding RNA (ncRNA)-mediated macrophage polarization in allergic rhinitis (AR) have not been systematically understood. Here, we identified long non-coding RNA (lncRNA) MIR222HG as a key regulator of macrophage polarization and revealed its role in AR. Consistent with our bioinformatic analysis of GSE165934 dataset derived from the Gene Expression Omnibus (GEO) database, lncRNA-MIR222HG and murine mir222hg were downregulated in our clinical samples and animal models of AR, respectively. Mir222hg was upregulated in M1 macrophages and downregulated in M2 macrophages. The allergen-ovalbumin facilitated polarization of RAW264.7 cells to the M2 phenotype, accompanied by the downregulation of mir222hg expression in a dose-dependent manner. Mir222hg facilitates macrophage M1 polarization and reverses M2 polarization caused by ovalbumin. Furthermore, mir222hg attenuates macrophage M2 polarization and allergic inflammation in the AR mouse model. Mechanistically, a series of gain- and loss-of-function experiments and rescue experiments were performed to verify the role of mir222hg as a ceRNA sponge that adsorbed miR146a-5p, upregulated Traf6, and activated the IKK/IκB/P65 pathway. Collectively, the data highlight the remarkable role of MIR222HG in the modulation of macrophage polarization and allergic inflammation, as well as its potential role as a novel AR biomarker or therapeutic target.

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“…For instance, in a study by Li et al focusing on dilated cardiomyopathy-induced CHF, lncRNA profiling identified 20 upregulated and 20 downregulated lncRNAs. Among them, the upregulated lncRNA RP11-544D21.2 and the downregulated lncRNA XLOC_014288 were correctly validated and functionally characterized [85] . In another investigation by Zhang et al, the lncRNA cytb was discovered to be downregulated in the heart and plasma of patients with heart failure [86] .…”

Section: Cardiovascular Diseasementioning

confidence: 99%

“…It stands as a prominent cause of mortality, particularly affecting the aging population, with an estimated lifetime risk of developing CHF ranging from 20% to 33%. Despite advancements in therapies, approximately half of CHF-diagnosed patients face a grim prognosis, with a significant number succumbing within five years [85] . Recent studies have brought attention to numerous aberrantly expressed lncRNAs in CHF, shedding light on their potential roles and significant functions in both the pathology and potential alleviation of the disease [85][86][87][88] .…”

Section: Cardiovascular Diseasementioning

confidence: 99%

“…Despite advancements in therapies, approximately half of CHF-diagnosed patients face a grim prognosis, with a significant number succumbing within five years [85] . Recent studies have brought attention to numerous aberrantly expressed lncRNAs in CHF, shedding light on their potential roles and significant functions in both the pathology and potential alleviation of the disease [85][86][87][88] . For instance, in a study by Li et al focusing on dilated cardiomyopathy-induced CHF, lncRNA profiling identified 20 upregulated and 20 downregulated lncRNAs.…”

Section: Cardiovascular Diseasementioning

confidence: 99%

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Silent players, loud impact: unveiling the therapeutic potentials of LncRNAs

Kamal,

Swellam,

Shalaby

et al. 2024

J Transl Genet Genom

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Long non-coding RNAs (lncRNAs) are a class of RNA transcripts that are long (i.e., more than 200 nucleotides) and not translated into proteins. They have recently emerged as potential treatment targets for numerous disorders due to their involvement in multiple cellular functions such as gene regulation, epigenetic modulation, and chromatin organization. This review highlights the current state of lncRNA-based therapeutics, the potential of lncRNAs as drug targets for treating human diseases, the various strategies and types of RNA-based therapeutics, and the complications of developing lncRNA-based drugs. We conclude that lncRNA-based therapeutics represent a promising class of drugs that can potentially treat various human diseases and that further research is needed to fully realize their therapeutic potential.

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Overexpression of cytosolic long noncoding RNA cytb protects against pressure-overload-induced heart failure via sponging microRNA-103-3p

Cited by 11 publications

References 84 publications

Identification of circular RNAs in cardiac hypertrophy and cardiac fibrosis

Identification of circular RNAs in cardiac hypertrophy and cardiac fibrosis

MIR222HG attenuates macrophage M2 polarization and allergic inflammation in allergic rhinitis by targeting the miR146a-5p/TRAF6/NF-κB axis

Silent players, loud impact: unveiling the therapeutic potentials of LncRNAs

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