Active Inducer Transport and Regulation of Microbial Enzyme Biosynthesis in Chemostat Cultures<sup>a</sup>

Ray, N. G.; Vieth, W. R.; Venκatasubramanian, Κ.

doi:10.1111/j.1749-6632.1986.tb26499.x

Cited by 2 publications

(2 citation statements)

References 37 publications

(1 reference statement)

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“…Model verification with lactose as the only carbon source has been reported earlier. 38 The presence of glucose in the growth medium alters the functions of lac operon control system, since glucose regulates some of the regulatory species which are involved in transcription control and inducer translocation. Therefore, modeling lac operon functions becomes more complex with the growth medium containing both glucose and lactose.…”

Section: Model Verificationmentioning

confidence: 99%

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Regulation of lac operon expression in mixed sugar chemostat cultures

Ray

Vieth

Venκatasubramanian

1987

Biotech & Bioengineering

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Expression of the lactose (lac) operon in the Escherichia coli chromosome has been studied in mixed-sugar chemostat cultures under steady-state and transient conditions. A unified model has been formulated which involves regulation of active inducer (lactose) transport, promoter-operator regulated expression of the lac operon, glucose-mediated inducer exclusion, and catabolite repression. The model of the lac operon control system focuses on the molecular interactions among the regulatory species and the genetic control elements for the initiation of transcription. The role of catabolite modulator factor (CMF) in the regulation of transcription is described. The modeling of glucose-mediated regulation of intracellular cyclic adenosine monophosphate (cAMP) and inducer exclusion is based on the recently elucidated mechanisms of the involvement of the PTS (phosphoen-olpyruvate dependent sugar transport system) enzymes, in the presence of glucose, in regulation of adenylate cyclase and non-PTS sugar transport proteins (i.e. per-meases). The adequacy of the unified model was verified with experimental data.

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Section: Model Verificationmentioning

confidence: 99%

“…This is quite different than what was observed with lactose alone. 38 Synthesis of lac operon enzymes is controlled at the transcription level. Any alterations in the regulatory elements may change the efficiency of the lac operon expression.…”

Section: Parameter Estimationmentioning

confidence: 99%

Regulation of lac operon expression in mixed sugar chemostat cultures

Ray

Vieth

Venκatasubramanian

1987

Biotech & Bioengineering

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The induction and repression of benzene and catechol oxidizing capacity of Pseudomonas putida ML2 studied in perturbed chemostat culture

Mason

1994

Arch. Microbiol.

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The oxidation of catechol, an intermediate in benzene catabolism, was studied using transient variations in dissolved oxygen tension (DOT) when a succinate limited steady state culture of Pseudomonas putida ML2 was perturbed with a pulse of another substrate. A model was developed and tested for the effect of fluctuations in oxidizing enzyme activity on DOT. It was found that the rate of induction of catechol oxidizing enzymes was independent of dilution rate up to a relative growth rate (mu/mumax) of 0.75. Only at higher dilution rates was catabolite repression observed.

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Active Inducer Transport and Regulation of Microbial Enzyme Biosynthesis in Chemostat Cultures^a

Cited by 2 publications

References 37 publications

Regulation of lac operon expression in mixed sugar chemostat cultures

Regulation of lac operon expression in mixed sugar chemostat cultures

The induction and repression of benzene and catechol oxidizing capacity of Pseudomonas putida ML2 studied in perturbed chemostat culture

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Active Inducer Transport and Regulation of Microbial Enzyme Biosynthesis in Chemostat Culturesa

Cited by 2 publications

References 37 publications

Regulation of lac operon expression in mixed sugar chemostat cultures

Regulation of lac operon expression in mixed sugar chemostat cultures

The induction and repression of benzene and catechol oxidizing capacity of Pseudomonas putida ML2 studied in perturbed chemostat culture

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Active Inducer Transport and Regulation of Microbial Enzyme Biosynthesis in Chemostat Cultures^a