The induction and repression of benzene and catechol oxidizing capacity of Pseudomonas putida ML2 studied in perturbed chemostat culture

Mason, Jeremy R.

doi:10.1007/bf00264373

Cited by 22 publications

(7 citation statements)

References 15 publications

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“…Although such observation has not been reported, it is in line with the previous statements that LMWOAs represented a readily utilizable C source for microorganisms (van Hees et al, 2002) and inhibited the degradation of aromatic compounds (Duetz et al, 1994;Mason, 1994;Dal et al, 2002). Dal et al (2002) have also reported that the consumption of aromatic compounds can be delayed in the presence of LMWOAs until the labile substrates have been depleted.…”

Section: Discussionsupporting

confidence: 74%

Effects of maize root exudates and organic acids on the desorption of phenanthrene from soils

Zhu

Zhang

Huang

et al. 2009

Journal of Environmental Sciences

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The effects of maize root exudates and low-molecular-weight-organic anions (LMWOAs) on the desorption of phenanthrene from eight artificially contaminated soils were evaluated. A significant negative correlation was observed between the amounts of phenanthrene desorbed and the soil organic carbon (SOC) contents (P < 0.01), and the influences of soil pH and clay content on phenanthrene desorption were insignificant (P > 0.1). Neither maize root exudates nor oxalate and citrate anions influenced desorption of phenanthrene with the addition of NaN 3 . A faster phenanthrene desorption occurred without the addition of NaN 3 in the presence of maize root exudates than oxalate or citrate due to the enhanced degradation by root exudates. Without the addition of NaN 3 , oxalate or citrate at different concentrations could inhibit phenanthrene desorption to different extents and the inhibiting effect by citrate was more significant than by oxalate. This study leads to the conclusion that maize root exudates can not enhance the desorption under abiotic condition with the addition of NaN 3 and can promote the desorption of phenanthrene in soils without the addition of NaN 3 .

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Section: Discussionsupporting

confidence: 74%

Effects of maize root exudates and organic acids on the desorption of phenanthrene from soils

Zhu

Zhang

Huang

et al. 2009

Journal of Environmental Sciences

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“…S8), which are typically involved in chloroaromatic, but also in nitroaromatic or hydroxybenzoquinol metabolism (Chapman and Ribbons, 1976;Kaschabek and Reineke, 1992;Perry and Zylstra, 2007). In accordance with the assumption that a huge set of aromatic pollutants may be degraded via catechol and intradiol cleavage (Mason, 1994;Filonov et al, 1997), a subset of intradiol pathway genes were expressed in the groundwater samples, specifically those for catechol metabolism. It is, however, also noteworthy that various aromatic catabolic genes, such as those for central aromatic metabolism (like the catechol ortho-cleavage pathway) or for benzoate metabolism (see above), obviously belong to the core genome of various proteobacterial (but also actinobacterial) genera (Perez-Pantoja et al, 2009a).…”

Section: Figmentioning

confidence: 87%

Analysis of the microbial gene landscape and transcriptome for aromatic pollutants and alkane degradation using a novel internally calibrated microarray system

Vílchez-Vargas

Geffers

Suárez-Diez

et al. 2012

Environmental Microbiology

132

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Despite various efforts to develop tools to detect and compare the catabolic potential and activity for pollutant degradation in environmental samples, there is still a need for an open-source, curated and reliable array method. We developed a custom array system including a novel normalization strategy that can be applied to any microarray design, allowing the calculation of the reliability of signals and make cross-experimental comparisons. Array probes, which are fully available to the scientific community, were designed from knowledge-based curated databases for key aromatic catabolic gene families and key alkane degradation genes. This design assigns signals to the respective protein subfamilies, thus directly inferring function and substrate specificity. Experimental procedures were optimized using DNA of four genome sequenced biodegradation strains and reliability of signals assessed through a novel normalization procedure, where a plasmid containing four artificial targets in increased copy numbers and co-amplified with the environmental DNA served as an internal calibration curve. The array system was applied to assess the catabolic gene landscape and transcriptome of aromatic contaminated environmental samples, confirming the abundance of catabolic gene subfamilies previously detected by functional metagenomics but also revealing the presence of previously undetected catabolic groups and specifically their expression under pollutant stress.

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“…Studies on P. putida ML-2 growing on succinate under carbon limitation in a continuous culture and sparged with catechol showed that a repression of catechol 2,3-dioxygenase activity was seen only at high dilution rates. The lack of repression of catechol 2,3-dioxygenase activity at lower dilution rates is believed to be due to the lower concentrations of succinate in the medium (18). P. putida CA-3 behaves differently in continuous culture than in batch culture with regard to the induction of the upper pathway by PAA.…”

Section: Discussionmentioning

confidence: 99%

The effect of nutrient limitation on styrene metabolism in Pseudomonas putida CA-3

O’Connor

Duetz

Wind

et al. 1996

Appl Environ Microbiol

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Styrene degradation in Pseudomonas putida CA-3 has previously been shown to be subject to catabolite repression in batch culture. We report here on the catabolite-repressing effects of succinate and glutamate and the effects of a limiting inorganic-nutrient concentration on the styrene degradation pathway of P. putida CA-3 in a chemostat culture at low growth rates (0.05 h ؊1). Oxidation of styrene and the presence of styrene oxide isomerase and phenylacetaldehyde dehydrogenase activities were used as a measure of the expression of the styrene degradation pathway. Both glutamate and succinate failed to repress the styrene degradation ability under growth conditions of carbon and energy limitation. Lower levels of enzyme activities of the styrene degradation pathway were seen in cells grown on styrene or phenylacetic acid (PAA) under conditions of both ammonia and sulfate limitation than were seen under carbon and energy limitation. Cells grown on PAA under continuous culture oxidize styrene and styrene oxide and possess styrene oxide isomerase and NAD ؉-dependent phenylacetaldehyde dehydrogenase activities. Catabolite repression of styrene metabolism was observed in cells grown on styrene or PAA in the presence of growth-saturating (nonlimiting) concentrations of succinate or glutamate under sulfate limitation.

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The induction and repression of benzene and catechol oxidizing capacity of Pseudomonas putida ML2 studied in perturbed chemostat culture

Cited by 22 publications

References 15 publications

Effects of maize root exudates and organic acids on the desorption of phenanthrene from soils

Effects of maize root exudates and organic acids on the desorption of phenanthrene from soils

Analysis of the microbial gene landscape and transcriptome for aromatic pollutants and alkane degradation using a novel internally calibrated microarray system

The effect of nutrient limitation on styrene metabolism in Pseudomonas putida CA-3

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