Active Immunization against Inhibin Improves Superovulatory Response to Exogenous FSH in Cattle

Takedomi, T.; Kishi, Hisashi; Medan, Mohamed S.; Aoyagi, Yoshito; Konishi, Masato; Itoh, Takuya; Yazawa, Shigeto; Watanabe, Gen; Taya, Kazuyoshi

doi:10.1262/jrd.16055

Cited by 30 publications

(17 citation statements)

References 32 publications

(28 reference statements)

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“…The yields of CL were increased gradually from 8.60±0.60 to 14.80±0.66 indicating a clear impact of FSH dose levels on CL production in Local genetic group (Table 1). The CL yields in Local genetic group with FSH observed in the present study were in agreement with the findings of Elsden et al (1978), Chupin and Procureur (1983), Yaakub et al (1999) and Takedomi et al (2005), but were higher than those reported by Sugano and Shinogi (1999). The yields of both the recovered embryos (RE) and transferable embryos (TE) showed significantly highest yields in the 280 mg of FSH dose level and thereafter decreased gradually indicating significant impact of 280 mg dose level of FSH on the production of transferable embryos in Local genetic group relating to dose specific response which might be influenced by the genotype and body weight of the experimental donor cows and the day of superovulation induction.…”

Section: Resultssupporting

confidence: 92%

Ovarian Response to Different Dose Levels of Follicle Stimulating Hormone (FSH) in Different Genotypes of Bangladeshi Cattle

Ali¹,

Khandoker²,

Afroz³

et al. 2011

Asian Australas. J. Anim. Sci

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The experiment was conducted under the Department of Animal Breeding and Genetics, Bangladesh Agricultural University (BAU), Mymensingh from June, 2001 to December, 2005 in two different locations (Central Cattle Breeding and Dairy Farm and Bangladesh Livestock Research Institute in Savar, Dhaka) to observe ovarian response to different doses of FSH in three different genotypes of cattle- indigenous Local, Pabna cattle and Friesian×Local cross. Five different dose levels used were 200, 240, 280, 320 and 360 mg. Ovarian response as corpus luteum (CL), recovered embryo (RE) and of transferable embryos (TE) count in Local were significant for 320, 280 and 280 mg respectively. In Pabna cattle CL, RE and TE count were found significant for 360, 320 and 320 mg respectively. In Friesian×Local cross CL, RE and TE count were found significant for 360, 320 and 320 mg respectively. The excellent quality embryos showed significantly the highest yield (1.80±0.20) in the 240 and 280 mg FSH levels in Local genotype. In Pabna cattle, the highest yield (2.00±0.32) was found at FSH level 320 mg. In Friesian×Local, the highest yield (2.20±0.20) was found at FSH level 280 mg.

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Section: Resultssupporting

confidence: 92%

Ovarian Response to Different Dose Levels of Follicle Stimulating Hormone (FSH) in Different Genotypes of Bangladeshi Cattle

Ali¹,

Khandoker²,

Afroz³

et al. 2011

Asian Australas. J. Anim. Sci

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“…Therefore, use of passive or active immunization against inhibin is a suitable, simple, and practical alternative to eCG for induction of superovulation in goats. Moreover, active i m m u n i z a t i o n a g a i n s t i n h i b i n i m p r o v e s superovulatory response to exogenous FSH in cattle [27]. In the present study, the ovulation rate was not comparable to the follicular population.…”

Section: Discussioncontrasting

confidence: 73%

“…Multiple ovulations have been induced successfully by passive immunization against endogenous inhibin in several species, such as rats [1], ewes [19,20], hamsters [12], cows [21,22], mares [10], guinea pigs [13], mice [23], and goats [24,25]. Also, active immunization against inhibin has been used in cows [26][27][28], goats [29], ewes [30], and mares [31]. T h u s , t h e s e i n i t i a l r e s u l t s i n d i c a t e t h a t immunization of animals against endogenous inhibin to induce superovulation through increased endogenous FSH secretion is an alternative method to the current exogenous gonadotropin protocols.…”

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confidence: 99%

Immunization of Goats against Inhibin Increased Follicular Development and Ovulation Rate

Sasaki

Medan

Watanabe

et al. 2006

Journal of Reproduction and Development

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Abstract. In the present study, two experiments were conducted to induce superovulation in goats using passive and active immunization against inhibin. In the first experiment, two groups of goats were given an intravenous injection of either 10 ml normal goat serum (control; n=6) or inhibin antiserum developed against [Tyro 30 ]-inhibin α (1-30) (passively immunized; n=6) 48 h before treatment with PGF2α. In the second experiment, two groups of goats were immunized with inhibin vaccine (actively immunized; n=5) or Freund's adjuvant (control; n=5) followed by three booster immunizations at 4 week intervals. Blood samples were collected for determination of FSH, LH, estradiol-17β, and progesterone. Ultrasonography was used to determine ovarian activity at PGF2α injection and ovulation rate one week after estrus. In both experiments, there was a significant increase in plasma FSH concentration compared with the controls. However, the pattern of the FSH levels was different between the passively and actively immunized goats. The numbers of follicles in passively and actively immunized goats (22.4 ± 2.3 and 18.6 ± 2.1, respectively) were significantly greater than those in the controls (2.6 ± 0.4 and 2.3 ± 0.4, respectively). In addition, the ovulation rate was greater in the immunized animals compared with the controls. Therefore, either passive or active immunization against inhibin could be used to induce superovulation in goats. Key words: Goats, Inhibin, Ovulation rate, Passive and active immunization (J. Reprod. Dev. 52: [543][544][545][546][547][548][549][550] 2006) nhibin is a heterodimeric glycoprotein hormone that selectively inhibits secretion of follicle stimulating hormone (FSH) from the pituitary gland [1]. A negative relationship between plasma concentrations of FSH and inhibin has been established in several mammalian species [2,3]. The number of follicles that develop to ovulatory size depends on both the amount of FSH and the time of exposure to FSH [4,5]. The release of FSH by the pituitary is in turn controlled by synergistic action of two of the major products of ovulatory follicles, inhibin and estradiol [6,7]. Previous findings [8][9][10] that immunoneutralization of endogenous inhibin produced a significant elevation of peripheral FSH offer evidence that inhibin is an important factor in the inhibitory regulation of FSH secretion in domestic animals as well as laboratory animals [11][12][13]. On the other hand, ultrasonographic observation of the ovary

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“…Also, although Inhbb expression was up‐regulated after Inha silencing, the secretion of Inhibin β is still decreased (Han et al, ), indicating how important their co‐production is. Interestingly, inhibin vaccines that attenuate its activity resulted in higher fertility and ovulation rates in sheep, cattle, and rats (Dan, Liu, Han, Liu, & Yang, ; Han et al, ; Takedomi et al, ). Activin, on the other hand, enhances the secretion of FSH, and is a promoter of FSHR expression within pituitary cells (Thackray, Mellon, & Coss, ).…”

Section: Discussionmentioning

confidence: 99%

The role of Melatonin receptor MTNR1A in the action of Melatonin on bovine granulosa cells

Wang

Liu

Wang

et al. 2017

Molecular Reproduction Devel

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Granulosa cells (GCs) play an important role in ovarian follicle growth, development, and follicular atresia. In the present study, we investigated the effects of Melatonin on bovine GCs, and asked if MTNR1A was involved in their response to this indole hormone. Our results indicated that Melatonin inhibited GC apoptosis by up-regulating the expression of BCL2, BCL-XL, GPX4, and SOD1, and down-regulating the expression of BAX, CASP3, and TP53. Moreover, Melatonin modulated bovine GC function by decreasing the expression of INHA, INHBB, FSHR, and TGFBR3, and the abundance of Inhibin β and Activin B, while increasing the expression of LHR, INHBA, and secretion of progesterone by GCs. In contrast, knockdown of MTNR1A significantly increased the expression of BAX, CASP3, TP53, INHA, FSHR, and TGFBR3, as well as Inhibin β abundance, while decreasing the expression of BCL2, GPX4, SOD1, and LHR, and production of progesterone and estradiol; no effect was observed on the expression of BCL-XL, INHBA, or INHBB. These results suggest that Melatonin and MTNR1A play an important role in modulating bovine GC function by regulating cellular progression, apoptosis, hormones secretion, and reproduction-related genes. Furthermore, altered expression of MTNR1A could affect how bovine GCs respond to Melatonin.

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Active Immunization against Inhibin Improves Superovulatory Response to Exogenous FSH in Cattle

Cited by 30 publications

References 32 publications

Ovarian Response to Different Dose Levels of Follicle Stimulating Hormone (FSH) in Different Genotypes of Bangladeshi Cattle

Ovarian Response to Different Dose Levels of Follicle Stimulating Hormone (FSH) in Different Genotypes of Bangladeshi Cattle

Immunization of Goats against Inhibin Increased Follicular Development and Ovulation Rate

The role of Melatonin receptor MTNR1A in the action of Melatonin on bovine granulosa cells

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