Active cellular sensing with quantum dots: Transitioning from research tool to reality; a review

Delehanty, James B.; Susumu, Kimihiro; Manthe, Rachel L.; Algar, W. Russ; Medintz, Igor L.

doi:10.1016/j.aca.2012.05.032

Cited by 71 publications

(51 citation statements)

References 195 publications

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“…The application of nanoparticle biosensors as active cellular probes is a highly promising area, and they are beginning to achieve success ( Fig. 5A) (78,82). Getting nanoparticles into intracellular regions of interest is a major challenge.…”

Section: Moving Toward Applicationsmentioning

confidence: 99%

Colloidal nanoparticles as advanced biological sensors

Howes

Chandrawati

Stevens

2014

Science

878

686

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Colloidal nanoparticle biosensors have received intense scientific attention and offer promising applications in both research and medicine. We review the state of the art in nanoparticle development, surface chemistry, and biosensing mechanisms, discussing how a range of technologies are contributing toward commercial and clinical translation. Recent examples of success include the ultrasensitive detection of cancer biomarkers in human serum and in vivo sensing of methyl mercury. We identify five key materials challenges, including the development of robust mass-scale nanoparticle synthesis methods, and five broader challenges, including the use of simulations and bioinformatics-driven experimental approaches for predictive modeling of biosensor performance. The resultant generation of nanoparticle biosensors will form the basis of high-performance analytical assays, effective multiplexed intracellular sensors, and sophisticated in vivo probes.Evolution has given rise to organisms of staggering complexity. Our now extensive knowledge of biological systems pales in comparison to the remaining mysteries. Unraveling these requires tools that probe the molecular machinery of life and provide detailed feedback on complex networks of subtle interactions. Such tools are cornerstones of biomedical research and practice, and improvements in these lead directly to a better understanding of fundamental biology, monitoring of health, and diagnosis of disease. Colloidal nanoparticle biosensors are a class of biological probe that will not only yield improved biological sensing but also provide a step change in our ability to probe the biomolecular realm. Nanoparticles can act as high-performance sensors because nanomaterials exhibit unique and useful behaviors not present in their bulk form: for example, bright tunable fluorescence from semiconductor nanoparticles and localized surface plasmon resonance (LSPR) phenomena in metallic nanoparticles. These particles exhibit intense responses to incident light (or other stimuli), and the ability to modulate this response by interaction with target analytes makes them excellent biosensor signal transducers. Outputs can be quantitative or qualitative depending on the functionality of

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Section: Moving Toward Applicationsmentioning

confidence: 99%

Colloidal nanoparticles as advanced biological sensors

Howes

Chandrawati

Stevens

2014

Science

878

686

View full text Add to dashboard Cite

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“…In the studies of living cells and in operation with in vivo animal models they bring unprecedented sensitivity and spatial resolution (Smith et al 2006 ). Combined with biomolecular engineering strategies for tailoring the particle surfaces at the molecular level, the bio-conjugated quantum dot probes are well suited for imaging the singlemolecule dynamics in living cells, for monitoring protein-protein interactions within specifi c intracellular locations, and for detecting diseased sites and tissues (Delehanty et al 2012 ). The explosive increase in the use of QDs in biological imaging was triggered by an improved synthesis of water-stable QDs, the development of approaches to introduce them effi ciently into the cells, and the improvements in conjugating QDs to specifi c biomolecules.…”

Section: The Quantum Dots Applications In Imagingmentioning

confidence: 99%

Sensing Inside the Living Cells

Demchenko

2015

Introduction to Fluorescence Sensing

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“…The ligand for the biological applications should possess a strong affinity for the QD surface. Ligand exchange is the method used to replace the original hydrophobic surface ligands on the QD surface with hydrophilic ligands or to attach to the QD a cell-penetrating peptide in order to allow QDs penetration into the living cells [15,16]. Thiol groups as in Fig.…”

Section: Synthesismentioning

confidence: 99%

Group III–V and II–VI Quantum Dots and Nanoparticles

Guda

Soldatov

Солдатов

2014

Springer Series in Optical Sciences

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By decreasing the size of semiconducting material the novel properties appear from the increased surface/bulk ratio and quantum confinement effects. X-ray absorption spectroscopy (XAS) can be applied to quantum dots and nanoparticles in-situ in the colloid, on the substrate or inside a solid matrix. The structural information about average size, phase composition, and growth orientation can be extracted from XAS along with information about electronic and magnetic properties of pure and doped nanostructures. In the chapter we shall describe the general properties of quantum dots and their applications that will help to understand the tasks for spectroscopy. Case studies will provide the information that is obtained from XAS complementary to other methods. Properties and Applications of Quantum DotsNanoparticle (NP) and quantum dot (QD) are regarded as zero-dimensional nanostructures. The term "QD" is usually used for semiconductor nanoparticles and islands where quantum confinement of electrons and excitons determines their properties. Decades passed after the dependence of the spectral position of the exciton absorption lines of the nanosized CuCl crystals was attributed to the quantum size effect [1] and three-dimensionally confined semiconductor quantum wells or zero-dimensional semiconductor nanostructures showing discrete electronic states were named "QDs"

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Active cellular sensing with quantum dots: Transitioning from research tool to reality; a review

Cited by 71 publications

References 195 publications

Colloidal nanoparticles as advanced biological sensors

Colloidal nanoparticles as advanced biological sensors

Sensing Inside the Living Cells

Group III–V and II–VI Quantum Dots and Nanoparticles

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