Active carbon uptake in Laminaria digitata and L. saccharina (Phaeophyta) is driven by a proton pump in the plasma membrane

Klenell, Markus; Snoeijs, Pauli; Pedersén, Marianne

doi:10.1023/b:hydr.0000018205.80186.3e

Cited by 46 publications

(27 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In addition, the VAN-sensitive carbon uptake (i.e. the mechanism of the carbon uptake based on the plasma membrane P-type H þ -ATPase activity) would definitely be inhibited also by TRIS buffer (Klenell et al, 2004), but no effect of TRIS buffer was found in the present study with E. binghamiae. Therefore, it is possible that VAN may inhibit photosynthesis simply because functional proton pumps are vital for cell function and thereby VAN could interfere with net photosynthesis more directly and not by means of any HCO À 3 acquisition mechanism.…”

contrasting

confidence: 48%

“…(Axelsson et al, 2000;Klenell et al, 2002Klenell et al, , 2004Mercado et al, 2006) and other macrophytes (Price & Badger, 1985;Hellblom et al, 2001;Hellblom & Axelsson, 2003;Uku et al, 2005). That is, a proton-gradient-driven CO 2 pump in the cell membrane based on the proton extrusion forming low pH (acid zones) on the thalli surface is integrated with HCO À 3 dehydration via external CA activity.…”

mentioning

confidence: 99%

“…It has been demonstrated to inhibit the HCO À 3 utilizing mechanism in macroalgae like the buffer sensitive brown alga Laminaria sp. (Klenell et al, , 2004, the red alga Coccotylus truncatus and the green alga Cladophora glomerata , most likely via inhibition of proton pumps. From the inhibitory effect of VAN on the photosynthetic rates, one might easily anticipate that a P-type H þ -ATPase (proton pump) was involved in carbon acquisition of E. binghamiae in natural seawater.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Acquisition of inorganic carbon byEndarachne binghamiae(Scytosiphonales, Phaeophyceae)

Zou

Gao

2010

European Journal of Phycology

View full text Add to dashboard Cite

Photosynthetic acquisition of inorganic carbon was studied in the brown seaweed Endarachne binghamiae J. Agardh. Photosynthesis was saturated at 245 mmol photons m À2 s À1 and photoinhibition did not occur at an irradiance as high as 750 mmol photons m À2 s À1 . The dependence of O 2 evolution on inorganic carbon (Ci) concentration demonstrated that the normal Ci composition in natural seawater was not saturating for irradiance-saturated photosynthesis. Three lines of evidence demonstrated that E. binghamiae was able to acquire HCO À 3 as a source of Ci for photosynthesis: (i) the high value of photosynthetic conductance for CO 2 (220.6 mm s À1 ); (ii) the high pH compensation point of 9.7; and (iii) the measured photosynthetic rates being in excess of the theoretical maximum rates supported solely by the CO 2 supply from the spontaneous dehydration of HCO À 3 in the bulk seawater. In order to establish the mechanism of Ci acquisition, specific inhibitors and a proton buffer were applied to examine their inhibitory effects on photosynthesis. No inhibitory effects were found for the proton buffer tris(hydroxymethyl)aminomethane and the anion exchanger inhibitor, 4,4 0 -diisothiocyano-stilbene-2,2 0 -disulphonate. By contrast, photosynthetic O 2 evolution in natural seawater was significantly depressed by the extracellular carbonic anhydrase (CA) activity inhibitor, acetazolamide, and the plasma membrane P-type H þ -ATPase inhibitor, vanadate.These results suggested that carbon acquisition from the natural seawater was mostly through the external CA-mediated HCO À 3 dehydration mechanism, and that P-type H þ -ATPase (proton pump) in the plasma membrane simultaneously functioned in photosynthesis of E. binghamiae. Additional experiments on the O 2 exchange versus pH value relationship indicated that, in contrast to photosynthesis, dark respiration of E. binghamiae was insensitive to the change of pH in the seawater, which resulted in a decreasing instantaneous balance between net carbon gain and respiratory carbon loss at high pH values in seawater.

show abstract

contrasting

confidence: 48%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Acquisition of inorganic carbon byEndarachne binghamiae(Scytosiphonales, Phaeophyceae)

Zou

Gao

2010

European Journal of Phycology

View full text Add to dashboard Cite

show abstract

“…7). However, caution should be exercised when interpreting these inhibition rates, as the AZ inhibitor influences the buffering capacity of seawater, and thus the rates of the pH increase (Klenell et al 2004).…”

Section: Resultsmentioning

confidence: 99%

Is the tetrasporophyte of Asparagopsis armata (Bonnemaisoniales) limited by inorganic carbon in integrated aquaculture?¹

Mata

Silva

Schuenhoff

et al. 2007

Journal of Phycology

View full text Add to dashboard Cite

Seaweeds cultivated in traditional land-based tank systems usually grow under carbon-limited conditions and consequently have low production rates, if no costly artificial source of inorganic carbon is supplied. In integrated aquaculture, the fish effluents provide an extra source of dissolved inorganic carbon (DIC) to seaweeds due to fish respiration. To evaluate if the tetrasporophyte of Asparagopsis armata (Harv.) F. Schmitz (the Falkenbergia stage) is carbon limited when cultivated with effluents of a fish (Sparus aurata) farm in southern Portugal, we characterized the DIC forms in the water, assessed the species photosynthetic response to the different DIC concentrations and pH values, and inferred for the presence of a carbonic anhydrase (CA)-mediated mechanism. Results showed that A. armata relies mainly on CO 2 to meet photosynthetic needs. Nevertheless, from pH 7.5 upward, the CO 2 supply to RUBISCO seems to derive also from the external dehydration of HCO 3 -mediated by CA. The contribution of this mechanism was essential for A. armata to attain fully saturated O 2 -evolution rates at the natural seawater DIC concentration (2-2.2 mM) and pH values ($8.0). We revealed in this study that seaweeds cultivated in fish-farm effluents benefit not only from a rich source of ammonia but also from an important and free source of DIC for their photosynthesis. If supplied at relatively high turnover rates ($100 vol AE d )1 ), fish-farm effluents provide enough carbon to maximize the photosynthesis and growth even for species with low affinity for HCO 3 -, avoiding the artificial and costly supply of inorganic carbon to seaweed cultures.

show abstract

“…The model suggested that algal production accounted about 14-40% of total CO 2 variation in seaweed farm. 그동안 해조류에 의한 영양염과 이산화탄소 등의 흡수 연구는 광합성에 따른 해조류 체내의 미량원소 요구량 및 산소발생 측면 에서 서술되었으며, 이들 실험도 실내 배양을 통해 얻은 결과들이 대부분이다 (Ale et al, 2010;Klenell et al, 2004;Ozaki et al, 2001;Rees, 2007;Zou, 2005). 국내에서도 미역, 다시마를 비롯한 다양한 해조류에 대한 영양염 요구 및 이산화탄소 흡수량에 대한 연구 결과들이 최근 보고되고 있다 (Hwang et al, 2011;Shim et al, 2010;Zou, 2005 (Fig.…”

unclassified

Real-time Monitoring of Environmental Properties at Seaweed Farm and a Simple Model for CO₂Budget

Shim¹,

Kang

Han³

et al. 2012

The Sea

View full text Add to dashboard Cite

Real-time monitoring for environmental factors(temperature, salinity, chlorophyll, etc.) and carbonate components(pH and fCO 2 ) was conducted during 5-6th of July, 2012 at a seaweeds farm in Gijang, Busan. Surface temperature and salinity were ranged from 12.5~17.6 o C and 33.7~34.0, respectively, with highly daily and inter-daily variations due to tide, light frequency(day and night) and currents. Surface f CO 2 and pH showed a range of 381~402µatm and 8.03~8.15, and chlorophyll-a concentration in surface seawater ranged 0.8~5.8 µg L -1. Environmental and carbonate factors showed the highest/lowest values around 5 pm of 5th July when the lowest tidal height and strongest thermocline in the water column, suggesting that biological production resulted in decrease of CO 2 and increase of pH in the seaweed farm. Processes affecting the surface f CO 2 distribution were evaluated using a simple budget model. In day time, biological productions by phytoplankton and macro algae are the main factors for CO 2 drawdown and counteracted the amount of CO 2 increase by temperature and air-sea exchange. The model values were a little higher than observed values in night time due to the over-estimation of physical mixing. The model suggested that algal production accounted about 14-40% of total CO 2 variation in seaweed farm.

show abstract

Active carbon uptake in Laminaria digitata and L. saccharina (Phaeophyta) is driven by a proton pump in the plasma membrane

Cited by 46 publications

References 0 publications

Acquisition of inorganic carbon byEndarachne binghamiae(Scytosiphonales, Phaeophyceae)

Acquisition of inorganic carbon byEndarachne binghamiae(Scytosiphonales, Phaeophyceae)

Is the tetrasporophyte of Asparagopsis armata (Bonnemaisoniales) limited by inorganic carbon in integrated aquaculture?¹

Real-time Monitoring of Environmental Properties at Seaweed Farm and a Simple Model for CO₂Budget

Contact Info

Product

Resources

About

Active carbon uptake in Laminaria digitata and L. saccharina (Phaeophyta) is driven by a proton pump in the plasma membrane

Cited by 46 publications

References 0 publications

Acquisition of inorganic carbon byEndarachne binghamiae(Scytosiphonales, Phaeophyceae)

Acquisition of inorganic carbon byEndarachne binghamiae(Scytosiphonales, Phaeophyceae)

Is the tetrasporophyte of Asparagopsis armata (Bonnemaisoniales) limited by inorganic carbon in integrated aquaculture?1

Real-time Monitoring of Environmental Properties at Seaweed Farm and a Simple Model for CO2Budget

Contact Info

Product

Resources

About

Is the tetrasporophyte of Asparagopsis armata (Bonnemaisoniales) limited by inorganic carbon in integrated aquaculture?¹

Real-time Monitoring of Environmental Properties at Seaweed Farm and a Simple Model for CO₂Budget