Active absorption of ginsenoside Rg1 &lt;I&gt;in vitro&lt;/I&gt; and &lt;I&gt;in vivo&lt;/I&gt;: the role of sodium-dependent glucose co-transporter 1

Xiong, Jing; Sun, Minjie; Guo, Zhixiong; Huang, Luosheng; Wang, Shujing; Meng, Boyu; Ping, Qineng

doi:10.1211/jpp/61.03.0014

Cited by 18 publications

(11 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…After systematic review, no published article was found on the human use of the bioavailable Rg1 monomer. Several studies focused on its bioavailability in rats in vivo; in vitro studies demonstrated that Rg1 can be absorbed after oral gavage [18]. A preliminary study showed that ginsenoside Rg1 is metabolized into 20 ( S )-protopanaxatriol via ginsenoside Rh1 and ginsenoside F1 by the gut microbiota of humans and mice.…”

Section: Discussionmentioning

confidence: 99%

Chondroprotective Effects of Ginsenoside Rg1 in Human Osteoarthritis Chondrocytes and a Rat Model of Anterior Cruciate Ligament Transection

et al. 2017

View full text Add to dashboard Cite

This study aimed to assess whether Ginsenoside Rg1 (Rg1) inhibits inflammatory responses in human chondrocytes and reduces articular cartilage damage in a rat model of osteoarthritis (OA). Gene expression and protein levels of type II collagen, aggrecan, matrix metalloproteinase (MMP)-13 and cyclooxygenase-2 (COX-2) were determined in vitro by quantitative real-time-polymerase chain reaction and Western blotting. Prostaglandin E2 (PGE2) amounts in the culture medium were determined by enzyme-linked immunosorbent assay (ELISA). For in vivo assessment, a rat model of OA was generated by anterior cruciate ligament transection (ACLT). Four weeks after ACLT, Rg1 (30 or 60 mg/kg) or saline was administered by gavage once a day for eight consecutive weeks. Joint damage was analyzed by histology and immunohistochemistry. Ginsenoside Rg1 inhibited Interleukin (IL)-1β-induced chondrocyte gene and protein expressions of MMP-13, COX-2 and PGE2, and prevented type II collagen and aggrecan degradation, in a dose-dependent manner. Administration of Ginsenoside Rg1 to OA rats attenuated cartilage degeneration, and reduced type II collagen loss and MMP-13 levels. These findings demonstrated that Ginsenoside Rg1 can inhibit inflammatory responses in human chondrocytes in vitro and reduce articular cartilage damage in vivo, confirming the potential therapeutic value of Ginsenoside Rg1 in OA.

show abstract

Section: Discussionmentioning

confidence: 99%

Chondroprotective Effects of Ginsenoside Rg1 in Human Osteoarthritis Chondrocytes and a Rat Model of Anterior Cruciate Ligament Transection

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Transport of ginsenosides across the intestinal mucosa is energy-dependent and non-saturable [77-79]. The sodium-dependent glucose co-transporter 1 may be involved in this process [80]. The availability of intact ginsenosides and their metabolites from the intestines is extremely low [81-83].…”

Section: Reviewmentioning

confidence: 99%

Pharmacology of ginsenosides: a literature review

2010

View full text Add to dashboard Cite

The therapeutic potential of ginseng has been studied extensively, and ginsenosides, the active components of ginseng, are shown to be involved in modulating multiple physiological activities. This article will review the structure, systemic transformation and bioavailability of ginsenosides before illustration on how these molecules exert their functions via interactions with steroidal receptors. The multiple biological actions make ginsenosides as important resources for developing new modalities. Yet, low bioavailability of ginsenoside is one of the major hurdles needs to be overcome to advance its use in clinical settings.

show abstract

“…37−39 Caco-2 cells are known to express active transporters, including sugar transporters, such as SGLT1, GLUT2, and GLUT5. 10 A study by Liu et al also reported that a sugar transporter, SGLT1, is responsible for the transport of glycosides in Caco-2 cells. 40 Analyzing our permeability assay data with the literature reports in mind, it can be interpreted that the glycosylated flavonoids assessed here may be actively transported, possibly by sugar transporters across Caco-2 monolayers.…”

Section: ■ Results and Discussionmentioning

confidence: 98%

Caco-2 Cell Permeability of Flavonoids and Saponins from Gynostemma pentaphyllum: the Immortal Herb

Ahmed

Leach²,

Wohlmuth

et al. 2020

ACS Omega

View full text Add to dashboard Cite

Gynostemma pentaphyllum (the immortal herb) has been an important component of Chinese Traditional Medicine for millennia. Recent clinical studies have revealed that the plant exhibits numerous beneficial biological activities, making it of interest to the pharmaceutical industry. An extract of the herb contains over 200 individual secondary metabolites including flavonol glycosides and dammarane saponins. To focus attention on the compounds most likely to be responsible for the biological activities, this study predicts the potential oral bioavailability of nine dammarane saponins and five flavonol glycosides from G. pentaphyllum using the Caco-2 cell monolayer permeability model. Two flavonoids, 8 and 9 , and four saponins, 10 , 11 , 12 , and 14 , exhibited high permeability across the monolayers. The results indicated that a higher degree of glycosylation-facilitated permeability, suggestive of active transport. This study demonstrates the utility of the Caco-2 permeability assay as a method of identifying possible bioavailable compounds from medicinal herbal extracts.

show abstract

Active absorption of ginsenoside Rg1 <I>in vitro</I> and <I>in vivo</I>: the role of sodium-dependent glucose co-transporter 1

Abstract: The data suggested that adrenaline enhanced the absorption of Rg1 by regulating sodium-dependent glucose co-transporter 1.

Cited by 18 publications

References 30 publications

Chondroprotective Effects of Ginsenoside Rg1 in Human Osteoarthritis Chondrocytes and a Rat Model of Anterior Cruciate Ligament Transection

Chondroprotective Effects of Ginsenoside Rg1 in Human Osteoarthritis Chondrocytes and a Rat Model of Anterior Cruciate Ligament Transection

Pharmacology of ginsenosides: a literature review

Caco-2 Cell Permeability of Flavonoids and Saponins from Gynostemma pentaphyllum: the Immortal Herb

Contact Info

Product

Resources

About