Activation of the Ascorbate‐Glutathione Cycle Is an Early Response of Micropropagated <i>Vitis vinifera</i> L. Explants Transferred to <i>ex Vitro</i>

Carvalho, Luísa C.; Vilela, Belmiro; Vidigal, Patrícia; Mullineaux, Philip M.; Amâncio, Sara

doi:10.1086/503919

Cited by 34 publications

(65 citation statements)

References 57 publications

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“…H 2 O 2 accumulation induced the expression of CAT, APX1, and APX3, together with the MDHAR-GOR branch of the ascorbateglutathione cycle in both genotypes. This, together with similar results obtained for micropropagated grapevine subjected to high light (Carvalho et al, 2006) and of plants under viral infection (Sgherri et al, 2013) shows that, upon a severe stress, MDHAR alone is unable of maintaining the AsA pool in the reduced form, thus calling for the function of the whole ascorbate-glutathione cycle. After 3 days of acclimation to high light (Carvalho et al, 2006) or 24 h after the end of heat stress , the MDHAR branch of the cycle can keep the ascorbate pool reduced.…”

Section: The Ascorbate-glutathione Cyclesupporting

confidence: 73%

“…This, together with similar results obtained for micropropagated grapevine subjected to high light (Carvalho et al, 2006) and of plants under viral infection (Sgherri et al, 2013) shows that, upon a severe stress, MDHAR alone is unable of maintaining the AsA pool in the reduced form, thus calling for the function of the whole ascorbate-glutathione cycle. After 3 days of acclimation to high light (Carvalho et al, 2006) or 24 h after the end of heat stress , the MDHAR branch of the cycle can keep the ascorbate pool reduced. However, the activation of the ascorbate-glutathione cycle is not, on its own, a trustworthy indicator of oxidative stress, as its activity can be triggered by differentiation of emerging structures in developing plants (Carvalho and Amâncio, 2002;Carvalho et al, 2006).…”

Section: The Ascorbate-glutathione Cyclesupporting

confidence: 73%

“…Antioxidants determine the lifetime and the specificity of the ROS signal or processing products. In this process, enzymes such as SOD, CAT, Prx, APX, and GOR are the key players in antioxidant homeostasis (Carvalho et al, 2006;Vidigal et al, 2013). See Table 1 for reference values of enzyme activity in different grapevine varieties.…”

Section: Key Enzymes For Antioxidant Homeostasismentioning

confidence: 99%

“…In that category are included, among others, the metabolites ascorbic acid (AsA, also termed vitamin C), glutathione (GSH), and carotenoid pigments. The ROS signaling or degradation pathways depend on antioxidant redox buffering enzymes such as superoxide dismutase (SOD), catalase (CAT), peroxiredoxins (Prx), ascorbate peroxidase (APX), and glutathione reductase (GOR) (Carvalho et al, 2006;Vidigal et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

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Oxidative stress homeostasis in grapevine (Vitis vinifera L.)

Carvalho

Vidigal

Amâncio

2015

Front. Environ. Sci.

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Plants can maintain growth and reproductive success by sensing changes in the environment and reacting through mechanisms at molecular, cellular, physiological, and developmental levels. Each stress condition prompts a unique response although some overlap between the reactions to abiotic stress (drought, heat, cold, salt or high light) and to biotic stress (pathogens) does occur. A common feature in the response to all stresses is the onset of oxidative stress, through the production of reactive oxygen species (ROS). As hydrogen peroxide and superoxide are involved in stress signaling, a tight control in ROS homeostasis requires a delicate balance of systems involved in their generation and degradation. If the plant lacks the capacity to generate scavenging potential, this can ultimately lead to death. In grapevine, antioxidant homeostasis can be considered at whole plant levels and during the development cycle. The most striking example lies in berries and their derivatives, such as wine, with nutraceutical properties associated with their antioxidant capacity. Antioxidant homeostasis is tightly regulated in leaves, assuring a positive balance between photosynthesis and respiration, explaining the tolerance of many grapevine varieties to extreme environments. In this review we will focus on antioxidant metabolites, antioxidant enzymes, transcriptional regulation and cross-talk with hormones prompted by abiotic stress conditions. We will also discuss three situations that require specific homeostasis balance: biotic stress, the oxidative burst in berries at veraison and in vitro systems. The genetic plasticity of the antioxidant homeostasis response put in evidence by the different levels of tolerance to stress presented by grapevine varieties will be addressed. The gathered information is relevant to foster varietal adaptation to impending climate changes, to assist breeders in choosing the more adapted varieties and suitable viticulture practices.

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Section: The Ascorbate-glutathione Cyclesupporting

confidence: 73%

Section: The Ascorbate-glutathione Cyclesupporting

confidence: 73%

Section: Key Enzymes For Antioxidant Homeostasismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Oxidative stress homeostasis in grapevine (Vitis vinifera L.)

Carvalho

Vidigal

Amâncio

2015

Front. Environ. Sci.

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“…However, in pineapple plantlets the fresh weight increased significant during 45 days, presumably due the new environmental conditions and the photoautotrophic behavior of the plantlets. Generally, it is recognized that environmental change during in vitro to ex vitro conditions cause stress in plants, expressed in terms of accumulation of abscisic acid (ABA), proline and reactive oxygen species (CARVALHO et al, 2006;BATROVA;POSPOSILOVA;SYNKOVA, 2008). However, results on in vitro pineapple have shown that it is possible to affect the carbon metabolism by changing the environmental temperature during the light and the dark period (NIEVOLA et al, 2005).…”

Section: Resultsmentioning

confidence: 99%

Morpho-physiological changes in pineapple plantlets [Ananas comosus (L.) merr.] during acclimatization

et al. 2012

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Pineapple producing countries lack good quality propagation material to expand cultivars into new areas. Previously, research protocols may increase the offer of high quality plantlets and speed the introduction of new pineapple cultivars. The present work is to evaluate the morpho-physiological changes in plantlets of pineapple [Ananas comosus (L.) Merr. 'MD-2'] during the acclimatization phase. Plantlets were acclimatized under 80% relative humidity, 25.5 °C temperature and photosynthetic photon flux of 400-500 µmol m-2 s-1 as average for 45 d under natural photoperiods. All measurements (plant length, number of leaves and roots, fresh weight, width and length of leaf 'D', net photosynthesis and total transpiration rate) were carried out at the end of in vitro rooting phase coincident with 0 d of acclimatization and at 15, 30 and 45 d thereafter. Photosynthetic activity of in vitro plantlets did not increase during the first 30 d of the acclimatization phase. After 30 d, photosynthetic activity ranged from 5.72 to 9.36 µmol CO2 m-2 s-1 while total transpiration ranged from 6.0 to 1.42 mmol H2O m-2 s-1. During the first 30 days there were no significant differences in number of leaves, length or width of the longest ('D') leaf (cm) or plant length (cm). However, after 45 days plant fresh weight (g), length and width of the 'D' leaf (cm) and root number all increased significantly, while transpiration (mmol H2O m-2 s-1) declined. There were small but significant decreases in chlorophyll a and b (µg g-1 mf.). Increased photosynthetic activity after 30 d shows that the increase in light intensity and the reduction of relative humidity during acclimatization did not constitute inhibitory factors.

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Gain of function of stomatal movements in rooting Vitis vinifera L. plants: regulation by H2O2 is independent of ABA before the protruding of roots

Vilela

Carvalho²,

Ferreira

et al. 2007

Plant Cell Rep

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Reactive oxygen species (ROS), namely superoxide radical (O2(-)) and hydrogen peroxide (H(2)O(2)) are generated when plant tissues endure a variety of environmental stresses, including light stress. The extremely short life times of ROS makes the study of their production in planta very difficult. The use of ROS-specific tracer dyes, 3-3' diaminobenzidine and nitroblue tetrazolium, together with high-resolution imaging provides the opportunity to identify sites of photooxidative stress response by ROS accumulation. This technique was applied to grapevine during the first 7 days after transfer from in vitro to ex vitro under an irradiance 4-fold higher than in vitro. ROS accumulation was detected in the first days of analysis, which gradually decreased to levels comparable to greenhouse leaves. (O2(-)) was uniformly distributed while H(2)O(2) accumulated preferentially in veins, wounds and stomatal guard and surrounding cells. To evaluate the role of H(2)O(2 )in stomatal functioning and its crosstalk with abscisic acid (ABA) we focused on the percentage of coloured structures, stomatal aperture and ABA concentration. We propose that the high H(2)O(2) level triggered by increased light is responsible for the activation of a signalling pathway over stomatal cells, in a process apparently irrespective of ABA regulation prior to root protrusion. This could explain the gain of function of a low yet consistent percentage of stomatal cells, essential for plant survival during the ontogenic period in analysis.

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Activation of the Ascorbate‐Glutathione Cycle Is an Early Response of Micropropagated Vitis vinifera L. Explants Transferred to ex Vitro

Cited by 34 publications

References 57 publications

Oxidative stress homeostasis in grapevine (Vitis vinifera L.)

Oxidative stress homeostasis in grapevine (Vitis vinifera L.)

Morpho-physiological changes in pineapple plantlets [Ananas comosus (L.) merr.] during acclimatization

Gain of function of stomatal movements in rooting Vitis vinifera L. plants: regulation by H2O2 is independent of ABA before the protruding of roots

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