Activation of sperm motility in striped bass via a cAMP-independent pathway

He, Shuyang; Jenkins-Keeran, Karen; Woods, L. Curry

doi:10.1016/j.theriogenology.2003.08.015

Cited by 27 publications

(18 citation statements)

References 26 publications

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“…Sperm diluted in SIS containing 5·2 mM Ca 2+ , however, expressed decreased motility compared to lower concentrations suggesting that high calcium had a slight inhibitory influence on sperm motility. Such a finding is consistent with a Ca 2+ inhibitory effect in sperm of other species (Alavi et al , 2004 b ; He et al , 2004). Therefore, sperm exposed to elevated Ca 2+ were activated either on contact or during incubation despite the high osmolality SIS (260 mOsm kg −1 ).…”

Section: Discussionsupporting

confidence: 89%

Inhibitory effect of osmotic concentration, potassium and pH on motility of the sperm of the North American burbot Lota lota maculosa

Zuccarelli

Jensen

Ingermann

2007

Journal of Fish Biology

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Seminal plasma factors maintaining North American (NA) burbot Lota lota maculosa sperm quiescent were examined. Sperm were diluted into buffered saline solutions of various compositions and motility assessed. After 1 h in these solutions at 10°C, aliquots of the suspension were diluted with tap water and motility again assessed. Dilution of sperm in an incubation solution containing Ca 2þ in the absence of K þ initiated sperm motility resulting in low motility when sperm were subsequently diluted in tap water. Incubation solutions with osmolalities >200 mOsm kg ÿ1 and containing 12Á5 mM K þ prevented the onset of sperm motility and were associated with maximal sperm motility upon dilution in tap water. Sperm maintained at lower osmolalities exhibited limited motility upon dilution in tap water indicating interdependence between K þ and osmolality in maintaining sperm quiescent in the presence of Ca 2þ . Sperm kept in incubation solution at pH values < c. 7Á5 for 1 h demonstrated reduced motility when subsequently diluted in tap water. That motility of sperm was pH sensitive was further indicated by CO 2 inhibition of motility. Therefore, NA burbot sperm are probably maintained in an immotile state, yet with potential for motility, by combination of high K þ , osmolality and possibly pH. The results from this study differ from published information on sperm quiescence in the temporally and geographically distinct Eurasian burbot Lota lota lota.

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Section: Discussionsupporting

confidence: 89%

Inhibitory effect of osmotic concentration, potassium and pH on motility of the sperm of the North American burbot Lota lota maculosa

Zuccarelli

Jensen

Ingermann

2007

Journal of Fish Biology

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“…In case of rainbow trout, chum salmon or sturgeon, flagellar axonemes need to be exposed to both cyclic AMP (cAMP) and ATP to become functionally motile [61,76,80,98,99]. However, this feature is not general as the presence of cAMP does not seem to be necessary for sperm motility initiation in many other fish species [100,101].…”

Section: Flagellum Wave Propagationmentioning

confidence: 99%

Biophysics of Fish Sperm Flagellar Movement: Present Knowledge and Original Directions

Prokopchuk¹,

Cosson²

2017

Cytoskeleton - Structure, Dynamics, Function and Disease

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A fish spermatozoon has a minimalist structure: head, mid-piece and flagellum with the active inner core, called "axoneme". The axoneme represents a cylindrical scaffold of microtubular doublets arranged around a pair of single microtubules and assorted along the entire length with the dynein-ATPase motors. The mechanisms of wave generation along the flagellum becomes possible due to sliding of microtubules relative to each other and their propagation is a result of a balance between mechanical constraints and intra-flagellar biochemical actors that generate force.How fish sperm flagella mechanics adapt to external constraints, such as vicinity of surfaces or viscosity, during the very short period of motility? By use of high-speed video microscopy, stroboscopic system, modelisation and simulation approaches, we show that fish sperm flagella respond to physical and chemical signals from environment in a very brief period of time.This review chapter presents a brief description of the biological and biochemical features that characterize fish spermatozoa. Then it describes the biophysical aspects of flagellar movement covering various topics involved in fish sperm motility and offering a compilation of the recent knowledge acquired on different physical properties, such as wave propagation, energetics, hydrodynamics, temperature, viscosity, axonemal microtubules dynamics, among other aspects.

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“…Previous studies have shown that several divalent ions have an influence similar to Ca 21 on sperm motility (Okuno and Morisawa, '89;He et al, 2004). To investigate this possibility in bluegill sperm, semen samples were diluted in SIS containing 1.25 or 2.5 mmol/L MnCl 2 , SrCl 2 , MgCl 2 , CdCl 2 or BaCl 2 .…”

Section: Divalent Cationsmentioning

confidence: 99%

“…The authors attributed this reduction to induction of asymmetric waveform causing sperm flagella to adopt a fishhook configuration similarly reported in sea urchin sperm (Gibbons and Gibbons,'80) and not to specific inhibition of motility. Ca 21 also induces partial inhibition of sperm motility in striped bass, however, only minor inhibition of sperm motility results, approximately 10% reduction at 10 mmol/L Ca 21 (He et al, 2004). Only recently have studies been conducted on the sperm of the Centrarchidae (Leach and Montgomerie, 2000;Neff et al, 2003;Burness et al, 2004Burness et al, , 2005Casselman and Montgomerie, 2004).…”

mentioning

confidence: 99%

Calcium‐induced quiescence of sperm motility in the bluegill (Lepomis macrochirus)

Zuccarelli¹,

Ingermann²

2007

J. Exp. Zool.

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Before dilution in hypoosmotic media sperm of freshwater fish are maintained quiescent by a range of factors including osmolality, K+ and pH, and the onset of motility is generally associated with an increase in cytoplasmic Ca2+. In contrast, Ca2+ in conjunction with osmolality was found to inhibit motility of intact bluegill sperm. Consistent with seminal plasma composition, 0.16 mmol/L Ca2+ and greater, in conjunction with an osmotic concentration of 290 mOsm/kg, inhibited the onset of bluegill sperm motility; sperm diluted in saline at 290 mOsm/kg without Ca2+ became motile. Cations Mn2+ and Sr2+, in conjunction with osmolality, had an inhibitory effect on initiation of sperm motility similar to that of Ca2+. Sperm motility was inhibited by Ca2+ channel blockers nimodipine and nifedipine, the mitochondrial Ca2+ uniporter inhibitor ruthenium red and the calmodulin inhibitors W-7 and trifluoperazine dihydrochloride. These results provide evidence that elevated cytoplasmic Ca2+ inhibits sperm motility and yet low levels permit or promote motility. This study demonstrates a unique inhibitory action of Ca2+ on the motility of intact fish sperm at physiologically relevant levels.

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Activation of sperm motility in striped bass via a cAMP-independent pathway

Cited by 27 publications

References 26 publications

Inhibitory effect of osmotic concentration, potassium and pH on motility of the sperm of the North American burbot Lota lota maculosa

Inhibitory effect of osmotic concentration, potassium and pH on motility of the sperm of the North American burbot Lota lota maculosa

Biophysics of Fish Sperm Flagellar Movement: Present Knowledge and Original Directions

Calcium‐induced quiescence of sperm motility in the bluegill (Lepomis macrochirus)

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