Activation of PPAR γ and δ by conjugated linoleic acid mediates protection from experimental inflammatory bowel disease

Bassaganya‐Riera, Josep; Reynolds, Kathryn; Martino-Catt, Susan; Cui, Yongzhi; Hennighausen, Lothar; Gonzalez, Frank J.; Rohrer, James E.; Benninghoff, Alejandro Uribe; Hontecillas, Raquel

doi:10.1053/j.gastro.2004.06.049

Cited by 355 publications

(373 citation statements)

References 73 publications

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“…Consistent with this theory, whole-body PPAR-␥ ϩ/Ϫ mice and immune cell-specific PPAR-␥ null mice are more susceptible to autoimmune and intestinal inflammatory diseases (34,37,42). Furthermore, PPAR-␥ activation by synthetic or natural agonists in an inflammatory setting limits the extent of tissue injury (10,11).…”

Section: Discussionmentioning

confidence: 85%

“…Tissue-specific PPAR-␥ fl/fl; MMTV-Cre ϩ (i.e., hemopoietic and epithelial cell-deficient) PPAR-␥ null mice and PPAR-␥ fl/fl; MMTV-Cre Ϫ littermates in a C57BL6/J background were generated by using the Cre-lox recombination system as previously described (37,38). The mice were maintained in the animal facilities at Virginia Polytechnic Institute and State University and used at 6 -8 wk.…”

Section: Micementioning

confidence: 99%

“…Previously, we have shown that conjugated linoleic acid, a polyunsaturated fatty acid, protects mice from DSS colitis through a mechanism dependent on the expression and activation of PPAR-␥ in epithelial and immune cells. In addition, PPAR-␥ mRNA expression was completely shut down in colons with severe inflammation (37). The intrarectal administration of TNBS represents a well-established model of CD4 ϩ T cell-induced colitis characterized by a predominant Th1 response induced by the excessive production of IL-12 by APC (39).…”

Section: Loss Of Colonic Ppar-␥ Expression Exacerbates Tnbs-induced Cmentioning

confidence: 99%

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Peroxisome Proliferator-Activated Receptor γ Is Required for Regulatory CD4+ T Cell-Mediated Protection against Colitis

Hontecillas

Bassaganya‐Riera

2007

The Journal of Immunology

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142

139

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Peroxisome proliferator-activated receptor (PPAR) γ activation has been implicated in the prevention of immunoinflammatory disorders; however, the mechanisms of regulation of effector and regulatory CD4+ T cell functions by endogenously activated PPAR-γ remain unclear. We have used PPAR-γ-deficient CD4+ T cells obtained from tissue-specific PPAR-γ null mice (i.e., PPAR-γ fl/fl; MMTV-Cre+) to investigate the role of endogenous PPAR-γ on regulatory T cell (Treg) and effector CD4+ T cell function. Overall, we show that the loss of PPAR-γ results in enhanced Ag-specific proliferation and overproduction of IFN-γ in response to IL-12. These findings correlate in vivo with enhanced susceptibility of tissue-specific PPAR-γ null mice to trinitrobenzene sulfonic acid-induced colitis. Furthermore, the transfer of purified PPAR-γ null CD4+ T cells into SCID recipients results in enteric disease. To test the assertion that the deficiency of PPAR-γ in Treg impairs their ability to prevent effector T cell-induced colitis, we performed cotransfer studies. These studies demonstrate that PPAR-γ-expressing, but not PPAR-γ null Treg, prevent colitis induced by transfer of naive CD4+ T cells into SCID recipients. In line with these findings, the production of IFN-γ by spleen and mesenteric lymph node-derived CD4+ T cells was down-regulated following transfer of PPAR-γ-expressing, but not PPAR-γ null, Treg. In conclusion, our data suggest that endogenous PPAR-γ activation represents a Treg intrinsic mechanism of down-regulation of effector CD4+ T cell function and prevention of colitis.

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Section: Discussionmentioning

confidence: 85%

Section: Micementioning

confidence: 99%

Section: Loss Of Colonic Ppar-␥ Expression Exacerbates Tnbs-induced Cmentioning

confidence: 99%

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Peroxisome Proliferator-Activated Receptor γ Is Required for Regulatory CD4+ T Cell-Mediated Protection against Colitis

Hontecillas

Bassaganya‐Riera

2007

The Journal of Immunology

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142

139

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“…Chronically high intracellular Ca 21 levels can activate calcineurin, which appears to be needed in the conversion to oxidative muscle phenotype (Bigard et al, 2000;Delling et al, 2000). Narkar et al (2008) found that peroxisome proliferator-activated receptor d (PPARd) agonist can increase oxidative myofibres in adult mice, and CLA can act as a ligand for some of PPARs family, such as PPARa, g and d (Meadus et al, 2002;Bassaganya-Riera et al, 2004). Furthermore, Chang (2007) reported that PGC-1a activation, in partnership with nuclear hormone receptors, including PPARs, could increase oxidative capacity in skeletal muscle.…”

Section: Discussionmentioning

confidence: 99%

Age-related changes and nutritional regulation of myosin heavy-chain composition in longissimus dorsi of commercial pigs

Men

Deng

et al. 2013

Animal

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The objective of this study is to investigate the age-related changes of and the effects of dietary conjugated linoleic acid (CLA) on muscle-fibre types in commercial pigs. We divided 25 crossbred male pigs into five age groups (7, 30, 60, 100 and 180 days) and 30 finishing pigs into two dietary groups (one fed a CLA-enriched diet and the other fed a control diet for 30 days). We analysed the composition (%) of myosin heavy-chain (MyHC) mRNA according to the absolute copies of each MyHC (I, IIa, IIb and IIx) mRNA, and the activities of succinate dehydrogenase (SDH) and malate dehydrogenase (MDH) in the longissimus muscle. From days 7 to 180, the MyHC I mRNA abundance and SDH and MDH activities presented a decreasing trend, the MyHC IIb mRNA abundance presented a steady trend and the MyHC IIa and IIx mRNA abundances presented an increasing trend. On day 30, MyHC I and IIb mRNA abundances were at their lowest (P , 0.05), and the MyHC IIa and IIx mRNA abundances were at their highest (P , 0.05). In the CLA group, the MyHC I mRNA abundance and the activities of SDH and MDH were improved in the longissimus muscle, whereas pressure loss, drip loss and average back fat depth significantly decreased (P , 0.01) and shear force significantly increased (P , 0.01). Loin eye area, feed conversion rate and meat colour showed some tendency to be improved. These results indicated that more oxidative fibres might convert to glycolytic fibres with increasing age or weight, and that the early developmental stage might be a key stage for this conversion. During the finishing stage, the proportion of oxidative fibres might be increased by dietary CLA supplementation, which may contribute to the water-holding capacity of meat. The results would provide an important basis for the application of muscle-fibre types in the improvement of pork quality.

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“…A inibição da ativação de NF-κB também foi observada em células da musculatura lisa da artéria coronária de humanos. A associação com a inibição da produção de prostaglandina resultante do metabolismo do ácido araquidônico via cicloxigenases, também observada nessas células, pode explicar o efeito anti-aterogênico do CLA observado in vivo [139][140][141] .…”

Section: Cla E Receptores Ativados Por Proliferadores De Peroxissomounclassified

Os efeitos do ácido linoléico conjugado no metabolismo animal: avanço das pesquisas e perspectivas para o futuro

2008

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Activation of PPAR γ and δ by conjugated linoleic acid mediates protection from experimental inflammatory bowel disease

Cited by 355 publications

References 73 publications

Peroxisome Proliferator-Activated Receptor γ Is Required for Regulatory CD4+ T Cell-Mediated Protection against Colitis

Peroxisome Proliferator-Activated Receptor γ Is Required for Regulatory CD4+ T Cell-Mediated Protection against Colitis

Age-related changes and nutritional regulation of myosin heavy-chain composition in longissimus dorsi of commercial pigs

Os efeitos do ácido linoléico conjugado no metabolismo animal: avanço das pesquisas e perspectivas para o futuro

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