Activation of Osteoblast-like MC3T3-E1 Cell Responses by Poly(Lactide).

Ikarashi, Yoshiaki; Tsuchiya, Toshie; Kaniwa, Masa-aki; Nakamura, Akitada

doi:10.1248/bpb.23.1470

Cited by 19 publications

(9 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Here, cell morphology revealed that cells grown on fibres had smaller projected areas than those on planar surfaces [ 88 ]. These results were confirmed by other authors [ 89 – 92 ]. Also other polymers such as PLGA have been shown to be effective in enhancing osteoblast differentiation in vitro [ 93 ].…”

Section: Cytocompatibility Of Micro- and Nanostructured Surfacessupporting

confidence: 92%

Significance of Nano- and Microtopography for Cell-Surface Interactions in Orthopaedic Implants

Jäger

Zilkens

Zanger

et al. 2007

Journal of Biomedicine and Biotechnology

131

View full text Add to dashboard Cite

Cell-surface interactions play a crucial role for biomaterial application in orthopaedics. It is evident that not only the chemical composition of solid substances influence cellular adherence, migration, proliferation and differentiation but also the surface topography of a biomaterial. The progressive application of nanostructured surfaces in medicine has gained increasing interest to improve the cytocompatibility and osteointegration of orthopaedic implants. Therefore, the understanding of cell-surface interactions is of major interest for these substances. In this review, we elucidate the principle mechanisms of nano- and microscale cell-surface interactions in vitro for different cell types onto typical orthopaedic biomaterials such as titanium (Ti), cobalt-chrome-molybdenum (CoCrMo) alloys, stainless steel (SS), as well as synthetic polymers (UHMWPE, XLPE, PEEK, PLLA). In addition, effects of nano- and microscaled particles and their significance in orthopaedics were reviewed. The significance for the cytocompatibility of nanobiomaterials is discussed critically.

show abstract

Section: Cytocompatibility Of Micro- and Nanostructured Surfacessupporting

confidence: 92%

Significance of Nano- and Microtopography for Cell-Surface Interactions in Orthopaedic Implants

Jäger

Zilkens

Zanger

et al. 2007

Journal of Biomedicine and Biotechnology

131

View full text Add to dashboard Cite

show abstract

“…Although the authors did not explain the reason of the observed effect, in our opinion, it is also in this case connected with the presence of cytotoxic tin compounds in high‐molecular polylactide. The fact that the concentration of released lactic acid is much higher during the degradation of polylactide with low molar mass in comparison with high‐molecular PLLA27 supports our opinion. The studies of the growth of calvaria osteoblast cell culture on the surface of membranes made of polylactide and polylactide grafted with atelocollagen have been also conducted.…”

Section: Introductionsupporting

confidence: 82%

“…Studies on cell response with the use of films formed from different polylactides27 supported our earlier suppositions. Two types of polylactides were used; one with a high molar mass ( M w = 137,000–270,000 Da) achieved during the synthesis initiated by tin compounds, and the other with a low molar mass ( M w = 5,000–20,000 Da) obtained through polycondensation, therefore without the use of tin compounds.…”

Section: Introductionsupporting

confidence: 76%

Interaction of cells with L‐lactide/glycolide copolymers synthesized with the use of tin or zirconium compounds

Czajkowska

Dobrzyński

Bero

2005

J Biomedical Materials Res

View full text Add to dashboard Cite

Comparative studies have been carried out on the viability of cells culture and production of collagen on lactide/glycolide copolymers synthesized using zirconium or tin initiators. Cell response of various cell types: fibroblast-like cells, osteoblast-like cells, monocyte/macrophage-like cells has been examined. Cell response depends on the kind of cells and the initiator used during the synthesis of the polymer. In a number of cases the cells grown on materials containing zirconium have been reported to have better survival (for example, Saos2 cells). An enhanced collagen production on these copolymers has been also observed.

show abstract

“…On the other hand, inexpensive poly(lactide) (PLA) scaffolds of various configurations have been generated and utilized with a wide range of cell types 5–11. For example, poly( L ‐lactide) (PLLA) has been demonstrated to be the preferred biomaterial for bone applications due to its biocompatibility, superior material properties, and slow degradation rate 12–19. It has also been reported that PLLA of both low and high molecular weights (i.e., M w = 5 × 10 4 and M w = 1 × 10 5 ) could enhance the differentiation of MC3T3‐E1 cells 20.…”

Section: Introductionmentioning

confidence: 99%

Functionalization of poly(L‐lactide) nanofibrous scaffolds with bioactive collagen molecules

Chiu

Liu

Hsiao

et al. 2007

J Biomedical Materials Res

View full text Add to dashboard Cite

The utilization of electrospun biodegradable scaffolds by fine-tuning their biofunctionalities through a simple mixing method was demonstrated in this study. Poly(L-lactide) (PLLA)-based scaffolds containing small amounts of bioactive collagen type I molecules were investigated for enhancements in cellular behavior. Electron microscopy revealed no topological alterations of the fibers in the collagen/PLLA scaffolds when compared with pure PLLA scaffolds. Cell attachment after 24 h was robust on collagen/PLLA scaffolds, with cytoskeletal analysis showing that the attached cells were aligned along the fibers assuming a spindle-shape appearance. Despite these morphological differences, gene expression analyses revealed no apparent alterations in mRNA levels of four genes involved in cell attachment across the various scaffolds. Although cell proliferation was not adversely affected, there were clear differences in cell penetration; after 1 week, cells migrated through 32 and 85% of PLLA and collagen/PLLA scaffolds, respectively. Mineralization of primary calvaria osteoblasts provided further evidence that collagen-containing electrospun PLLA scaffolds could sustain cell differentiation. Overall, the inclusion of collagen type I in even miniscule amounts (<1 wt %) within electrospun PLLA scaffolds could effectively modulate certain aspects of cellular behavior.

show abstract

Activation of Osteoblast-like MC3T3-E1 Cell Responses by Poly(Lactide).

Cited by 19 publications

References 1 publication

Significance of Nano- and Microtopography for Cell-Surface Interactions in Orthopaedic Implants

Significance of Nano- and Microtopography for Cell-Surface Interactions in Orthopaedic Implants

Interaction of cells with L‐lactide/glycolide copolymers synthesized with the use of tin or zirconium compounds

Functionalization of poly(L‐lactide) nanofibrous scaffolds with bioactive collagen molecules

Contact Info

Product

Resources

About