Significance of Nano- and Microtopography for Cell-Surface Interactions in Orthopaedic Implants

Jäger, Markus; Zilkens, Christoph; Zanger, Klaus; Krauspe, Rüdiger

doi:10.1155/2007/69036

Cited by 131 publications

(99 citation statements)

References 191 publications

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“…The latest trends include topographic surface modifications comprising nanosize structures and patterns, mostly due to the potential for precisely controlling biological responses by surface features in the same size range as biological recognition and signaling system components. 7,8 However, there is a lack of knowledge about the very basic mechanisms underlying the cellular sensitivity to substrate nanotopography, as well as the topographic parameters (eg, shape, size, distribution density) that are most relevant in this context. One significant challenge in the study of biological system interaction with nanoscale structures is the shortage of suitable nanofabrication approaches to create large areas of systematically variable nanostructures.…”

Section: Introductionmentioning

confidence: 99%

Osteogenic response of human mesenchymal stem cells to well-defined nanoscale topography in vitro

Peppo¹,

Agheli²,

Karlsson³

et al. 2014

IJN

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Background Patterning medical devices at the nanoscale level enables the manipulation of cell behavior and tissue regeneration, with topographic features recognized as playing a significant role in the osseointegration of implantable devices. Methods In this study, we assessed the ability of titanium-coated hemisphere-like topographic nanostructures of different sizes (approximately 50, 100, and 200 nm) to influence the morphology, proliferation, and osteogenic differentiation of human mesenchymal stem cells (hMSCs). Results We found that the proliferation and osteogenic differentiation of hMSCs was influenced by the size of the underlying structures, suggesting that size variations in topographic features at the nanoscale level, independently of chemistry, can be exploited to control hMSC behavior in a size-dependent fashion. Conclusion Our studies demonstrate that colloidal lithography, in combination with coating technologies, can be exploited to investigate the cell response to well defined nanoscale topography and to develop next-generation surfaces that guide tissue regeneration and promote implant integration.

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Section: Introductionmentioning

confidence: 99%

Osteogenic response of human mesenchymal stem cells to well-defined nanoscale topography in vitro

Peppo¹,

Agheli²,

Karlsson³

et al. 2014

IJN

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“…Subsequently, it became evident that the presence of well-defined, µm-and nm-scale, surface variations significantly alters the recruited cell behaviour (Clark et al, 1987;Clark et al, 1990;Meyle et al, 1993;Liao et al, 2003;Hamilton et al, 2005;Dalby et al, 2007;Jäger et al, 2007). At the µm-scale, ordered cues seem to simulate specific cell reactions such as cell migration and osteoblastic differentiation (Kaiser and Bruinink, 2004;Schneider et al, 2004;Bruinink et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

In vitro bioactivity of micro metal injection moulded stainless steel with defined surface features

Bitar

Friederici²,

Imgrund³

et al. 2012

eCM

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Micrometre-and nanometre-scale surface structuring with ordered topography features may dramatically enhance orthopaedic implant integration. In this study we utilised a previously optimised micron metal injection moulding (µ-MIM) process to produce medical grade stainless steel surfaces bearing micrometre scale, protruding, hemispheres of controlled dimensions and spatial distribution. Additionally, the structured surfaces were characterised by the presence of submicrometre surface roughness resulting from metal grain boundary formation. Following cytocompatibility (cytotoxicity) evaluation using 3T3 mouse fibroblast cell line, the effect on primary human cell functionality was assessed focusing on cell attachment, shape and cytoskeleton conformation. In this respect, and by day 7 in culture, significant increase in focal adhesion size was associated with the microstructured surfaces compared to the planar control. The morphological conformation of the seeded cells, as revealed by fluorescence cytoskeleton labelling, also appeared to be guided in the vertical dimension between the hemisphere bodies. Quantitative evaluation of this guidance took place using live cytoplasm fluorescence labelling and image morphometry analysis utilising both, compactness and elongation shape descriptors. Significant increase in cell compactness was associated with the hemisphere arrays indicating collective increase in focused cell attachment to the hemisphere bodies across the entire cell population. Micrometre-scale hemisphere array patterns have therefore influenced cell attachment and conformation. Such influence may potentially aid in enhancing key cellular events such as, for example, neo-osteogenesis on implanted orthopaedic surfaces.

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“…16 This present paper presents a solution to this problem using a simple selfassembly procedure (briefly overviewed in Figure 1) where gold nanoparticles are immobilized on a gold substrate and sintered onto the surface by a following washing step. The particles used for surface preparation in the present study are in a size range that has previously been reported to affect adsorption of plasma proteins.…”

Section: Introductionmentioning

confidence: 99%

Immune complement activation is attenuated by surface nanotopography

Hulander¹,

Lundgren²,

Berglin³

et al. 2011

IJN

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The immune complement (IC) is a cell-free protein cascade system, and the first part of the innate immune system to recognize foreign objects that enter the body. Elevated activation of the system from, for example, biomaterials or medical devices can result in both local and systemic adverse effects and eventually loss of function or rejection of the biomaterial. Here, the researchers have studied the effect of surface nanotopography on the activation of the IC system. By a simple nonlithographic process, gold nanoparticles with an average size of 58 nm were immobilized on a smooth gold substrate, creating surfaces where a nanostructure is introduced without changing the surface chemistry. The activation of the IC on smooth and nanostructured surfaces was viewed with fluorescence microscopy and quantified with quartz crystal microbalance with dissipation monitoring in human serum. Additionally, the ability of pre-adsorbed human immunoglobulin G (IgG) (a potent activator of the IC) to activate the IC after a change in surface hydrophobicity was studied. It was found that the activation of the IC was significantly attenuated on nanostructured surfaces with nearly a 50% reduction, even after pre-adsorption with IgG. An increase in surface hydrophobicity blunted this effect. The possible role of the curvature of the nanoparticles for the orientation of adsorbed IgG molecules, and how this can affect the subsequent activation of the IC, are discussed. The present findings are important for further understanding of how surface nanotopography affects complex protein adsorption, and for the future development of biomaterials and blood-contacting devices.

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Significance of Nano- and Microtopography for Cell-Surface Interactions in Orthopaedic Implants

Cited by 131 publications

References 191 publications

Osteogenic response of human mesenchymal stem cells to well-defined nanoscale topography in vitro

Osteogenic response of human mesenchymal stem cells to well-defined nanoscale topography in vitro

In vitro bioactivity of micro metal injection moulded stainless steel with defined surface features

Immune complement activation is attenuated by surface nanotopography

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