Activation of NF-κB may be necessary but is not sufficient for induction of H-2 antigens by TNF in J558L murine myeloma cells

Abstract

We have investigated the role of the transcription factor NF-kappa B in the induction of H-2 antigens by tumor necrosis factor (TNF) in murine J558L myeloma cells. An earlier report suggested that J558L cells may have a defect in NF-kappa B activation in response to some stimuli. Treatment of J558L cells with either TNF or lipopolysaccharide (LPS) resulted in nuclear translocation of NF-kappa B, as demonstrated by electrophoretic mobility shift assay. Both TNF and LPS activated the same NF-kappa B nuclear complexes, composed of the p50 and p65 subunits. LPS mediated a stronger and more sustained activation of NF-kappa B than TNF. In contrast, TNF induced higher levels of H-2 antigen surface expression than did LPS, suggesting that activation of NF-kappa B is not sufficient for optimal enhancement of H-2 expression. An inhibitor of NF-kappa B activation, pyrrolidinedithiocarbamate (PDTC), dramatically reduced the induction of H-2 antigen by TNF, supporting the view that NF-kappa B is required for TNF-induced H-2 antigen expression. Constitutive levels of H-2 antigen expression on the cell surface and of nuclear NF-kappa B also decreased after PDTC treatment. However, PDTC had a smaller inhibitory effect on LPS-induced NF-kappa B activation and H-2 antigen expression, suggesting that TNF and LPS activate NF-kappa B by somewhat different pathways.

Kinase Regulation of Human MHC Class I Molecule Expression on Cancer Cells

“…MHC-I mRNA expression is regulated through upstream enhancer elements, with involvement of the NF-κB transcription factor (43,44). MHC-I is also induced by TNF, IL1, IFNβ, and IFNγ, which upregulates HLA-A via the JAK/STAT pathway (28,29).…”

Differential regulation of TSG-14 expression in murine fibroblasts and peritoneal macrophages

“…Nuclear extracts were prepared as previously described with minor modifications [21,22]. Protein concentrations were quantified using the Bradford assay (Bio-Rad).…”

Regulação da atividade da glândula pineal por estimulação purinérgica.

“…Estímulos diversos induzem a ativação de proteínas quinase de I B (IKKs), que são quinases responsáveis por fosforilar as moléculas de I B e fazer com que estas sejam degradadas via proteassoma, permitindo a translocação nuclear do dímero NF-B (Verma et al, 1995;Hayden & Gosh, 2008 Wolchok et al, 1994;De Plaen et al, 2006;Jiang et al, 2008).…”

“…Conforme comentado, este composto impede a ligação do NF-κB às regiões promotoras do gene após a entrada no núcleo celular (ZieglerHeitbrock et al, 1993;Wolchok et al, 1994;De Plaen et al, 2006;Jiang et al, 2008). Neste estudo, as glândulas pineais foram pré-tratadas com PDTC e então estimuladas com isoproterenol, na presença ou na ausência de ADP.…”