Activation of myeloid dendritic cells by deoxynucleic acids from Cordyceps sinensis via a Toll-like receptor 9-dependent pathway

Xiao, Gang; Miyazato, Akiko; Abe, Yu; Zhang, Tiantuo; Nakamura, Kiwamu; Inden, Ken; Tanaka, Misuzu; Tanno, Daiki; Miyasaka, Tomomitsu; Takeda, Kiyoshi; Akira, Shizuo; Saijo, Shinobu; Iwakura, Yoichiro; Adachi, Yoshiyuki; Ohno, Naohito; Yamamoto, Noriaki; Kunishima, Hiroyuki; Hirakata, Yoichi; Kaku, Mitsuo; Kawakami, Kazuyoshi

doi:10.1016/j.cellimm.2010.04.006

Cited by 12 publications

(15 citation statements)

References 36 publications

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“…In addition, Zhang and coworkers recently reported that TLR9 signaling plays a critical role in protective immune responses to C. neoformans and in clearance of this fungal pathogen from the lungs (34). Similar findings have been reported regarding other fungal species, including Aspergillus fumigatus, Candida albicans, and Cordyceps sinensis (20,24,30).…”

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confidence: 66%

Toll-Like Receptor 9-Dependent Activation of Bone Marrow-Derived Dendritic Cells byURA5DNA from Cryptococcus neoformans

et al. 2012

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Cryptococcus neoformans is an opportunistic fungal pathogen that causes meningoencephalitis in immunocompromised patients. Recently, we reported that Toll-like receptor 9 (TLR9) is involved in host defense against C. neoformans: specifically, it detects the pathogen's DNA. In the present study, we aimed to elucidate the mechanisms underlying TLR9-mediated activation of innate immune responses by using the URA5 gene, which encodes a virulent component of this fungal pathogen. A PCRamplified 345-bp URA5 gene fragment induced interleukin-12 p40 (IL-12p40) production by bone marrow-derived dendritic cells (BM-DCs) in a TLR9-dependent manner. Similar activity was detected in the 5= 129-bp DNA fragment of URA5 and in a synthesized oligodeoxynucleotide (ODN) with the same sequence. Shorter ODN fragments, which contained GTCGGT or GAC-GAT but had only 24 or 21 bases, induced IL-12p40 production and CD40 expression by BM-DCs, but this activity vanished when the CG sequence was replaced by GC or when a phosphorothioate modification was introduced. IL-12p40 production caused by active ODN was strikingly enhanced by treatment with DOTAP, a cationic lipid that increases the uptake of DNA by BM-DCs, though DOTAP failed to induce IL-12p40 production by inactive ODN and did not affect the activity of an ODNcontaining canonical CpG motif. There was no apparent difference in intracellular trafficking between active and inactive ODNs. Finally, an extremely high dose of inactive ODN suppressed IL-12p40 production by BM-DCs that had been stimulated with active ODN. These results suggest that the C. neoformans URA5 gene activates BM-DCs through a TLR9-mediated signaling pathway, using a mechanism possibly independent of the canonical CpG motif.

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confidence: 66%

Toll-Like Receptor 9-Dependent Activation of Bone Marrow-Derived Dendritic Cells byURA5DNA from Cryptococcus neoformans

et al. 2012

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“…Previously, Cordyceps had been clinically proven to have various properties, such as anti-tumor, anti-inflammatory and anti-oxidation effects (Li et al, 2006;Jeong et al, 2010;Xiao et al, 2010). The results of the active ingredients in CMWM show an accumulation of a variety of functional/secondary metabolites, such as cordycepin, cordycepic acid, phenolic compounds, triterpenoid, flavonoid, ergosterol, adenosine and polysaccharides, which are important antioxidants and synergists; they could improve the antioxidative capacity and oxidative status in humans/animals.…”

Section: Discussionmentioning

confidence: 99%

“…The results of the active ingredients in CMWM show an accumulation of a variety of functional/secondary metabolites, such as cordycepin, cordycepic acid, phenolic compounds, triterpenoid, flavonoid, ergosterol, adenosine and polysaccharides, which are important antioxidants and synergists; they could improve the antioxidative capacity and oxidative status in humans/animals. Cordycepin and cordycepic acid (D-mannitol) have been used generally as a marker for quality control in the medicinal value of Cordyceps (Li et al, 2006;Das et al, 2010;Xiao et al, 2010). Yilmaz et al (2007) pointed out that cordycepic acid could be used to decrease intracranial pressure in rats, increase the content of peroxidase and glutathione peroxidase in rat blood and reduce the formation of malondialdehyde, a marker for oxidative stress, thereby reducing cell damage.…”

Section: Discussionmentioning

confidence: 99%

Effect of <i>Cordyceps Militaris </i>Waster Medium on Production Performance, Egg Traits and Egg Yolk Cholesterol of Laying Hens

et al. 2015

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Cordyceps militaris waster medium (CMWM) is a by-product of the edible portion of the fruiting body of Cordyceps fungi. This study was conducted to investigate the effects of the CMWM on the production performance, egg quality and egg yolk cholesterol of laying hens. An in vitro study showed that the reducing power of the CMWM extracts increased linearly with its concentration; at a concentration of 4.0 mg/mL, it reached a level of roughly 60% ascorbic acid at 0.5 mg/mL, while inhibition of lipid peroxidation of the CMWM extracts at 15.0 mg/mL reached nearly 50%. Sixty 22-week-old laying hens (Hendrix) were randomly allocated into 4 groups with 5 replicates, 3 birds per replicate. They were fed diets supplemented with the following levels of the CMWM: 0 (control group, corn-soybean meal), 5.0, 10.0, or 20.0 g/kg dried CMWM groups for 12 weeks, respectively. The results revealed that the 20.0 g/kg CMWM group exhibited significantly decreased feed conversion ratio (FCR) and increased egg white weight compared to the control group at 5-8 and 9-12 weeks. The supplemented CMWM groups showed significantly elevated egg mass in comparison to the control group at 5-8 and 9-12 weeks. There were no effects for egg yolk weight, egg shell weight, shell thickness and egg yolk color between the groups. Those groups which were supplemented with 10.0 and 20.0 g/kg dried CMWM showed significantly decreased egg cholesterol compared to the control group at 9-12 weeks. On the basis of these observations, we concluded that CMWM, a by-product of Cordyceps militaris, could be employed as a new feed additive that offers potential benefits for elevating egg mass and producing lower cholesterol eggs by laying hens.

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“…C. sinensis has a variety of pharmacological effects including anti-tumor, antiinflammatory and immunomodulatory (Chen et al, 1997;Kim et al, 2006;Kuo et al, 2001;Rao et al, 2007). Recently, many studies have reported that the water extracts and the components of C. sinensis can regulate the maturation and function of DCs (Li et al, 2009;Song et al, 2013;Song et al, 2011;Xiao et al, 2010). C. sinensis water extracts could activate iDCs by stimulating the expression of co-stimulatory molecules and pro-inflammatory cytokines, enhancing the ability of DC in the induction of allogeneic T cell proliferation, and reducing the endocytic ability of DCs (Li et al, 2009).…”

Section: Cordyceps Sinensismentioning

confidence: 99%

“…The further study showed that EPS decreased phosphorylated Janus kinase 2 (p-JAK2), p-STAT3 and the level of nuclear factor-κB (NF-κB) p105 in the cytoplasm, increased levels of NF-κB p65 in the nucleus (Song et al, 2013). Xiao et al (2010) showed that the treatment of C. sinensis DNA, which is extracted from the fungal bodies, dose-dependently increased the expression of CD40 and the production of IL-12 and TNF-α in BM-DCs through TLR9 signaling pathway, which is similar to the effects of CpG. These results demonstrated that C. sinensis extracts and its components could promote the activation of DCs.…”

Section: Cordyceps Sinensismentioning

confidence: 99%

The immunoregulatory effects of Chinese herbal medicine on the maturation and function of dendritic cells

Zhang

2015

Journal of Ethnopharmacology

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Activation of myeloid dendritic cells by deoxynucleic acids from Cordyceps sinensis via a Toll-like receptor 9-dependent pathway

Cited by 12 publications

References 36 publications

Toll-Like Receptor 9-Dependent Activation of Bone Marrow-Derived Dendritic Cells byURA5DNA from Cryptococcus neoformans

Toll-Like Receptor 9-Dependent Activation of Bone Marrow-Derived Dendritic Cells byURA5DNA from Cryptococcus neoformans

Effect of <i>Cordyceps Militaris </i>Waster Medium on Production Performance, Egg Traits and Egg Yolk Cholesterol of Laying Hens

The immunoregulatory effects of Chinese herbal medicine on the maturation and function of dendritic cells

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