Activation of Murine CD4+ and CD8+ T Lymphocytes Leads to Dramatic Remodeling of<i>N</i>-Linked Glycans

Comelli, Elena M.; Sutton-Smith, Mark; Qi, Yuanming; Amado, Margarida; Panico, Maria; Gilmartin, Tim; Whisenant, Thomas; Lanigan, Caroline; Head, Steven R.; Goldberg, David; Morris, Howard R.; Dell, Anne; Paulson, James C.

doi:10.4049/jimmunol.177.4.2431

Cited by 110 publications

(110 citation statements)

References 67 publications

(60 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Consequently, the future challenge will be to examine immune responses in primary T-cell subpopulations separated on the basis of ganglioside species. Mouse CD4 + T cells and CD8 + T cells activated by anti-CD3 antibody exhibit different profiles for N-linked glycans (26). In addition, differentially N-glycosylated TCR complexes have been observed in CD4 + T cells compared with CD8 + T cells, which might affect the functional avidity of TCR-transduced T cells (27,28).…”

Section: Discussionmentioning

confidence: 99%

CD4 and CD8 T cells require different membrane gangliosides for activation

Nagafuku

Okuyama²,

Onimaru³

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

113

View full text Add to dashboard Cite

Initial events of T-cell activation involve movement of the T-cell receptor into lipid rafts. Gangliosides are major components of lipid rafts. While investigating T-cell activation in ganglioside-deficient mice, we observed that CD4 + and CD8 + T cells required different ganglioside subsets for activation. Activation of CD4 + T cells from GM3 synthase-null mice, deficient in GM3-derived gangliosides, is severely compromised, whereas CD8 + T-cell activation is normal. Conversely, in cells from GM2/GD2 synthase-null mice, expressing only GM3 and GD3, CD4 + T-cell activation is normal, whereas CD8 + T-cell activation is deficient. Supplementing the cells with the corresponding missing gangliosides restores normal activation. GM3 synthase-null mice do not develop experimental asthma. Distinct expression patterns of ganglioside species in CD4 + T and CD8 + T cells, perhaps in uniquely functional lipid rafts, define immune functions in each T-cell subset. Control of ganglioside expression would offer a strategy targeting for specific T-cell subpopulations to treat immune diseases.

show abstract

Section: Discussionmentioning

confidence: 99%

CD4 and CD8 T cells require different membrane gangliosides for activation

Nagafuku

Okuyama²,

Onimaru³

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

113

View full text Add to dashboard Cite

show abstract

“…These variables can dramatically change during thymic-development and peripheral activation and differentiation of T cells. [5,6,18,[38][39][40][41].…”

Section: Galectin-glycoprotein Lattices In T Cell Functionsmentioning

confidence: 99%

Functions of cell surface galectin-glycoprotein lattices

Rabinovich

Toscano

Jackson

et al. 2007

Current Opinion in Structural Biology

350

311

View full text Add to dashboard Cite

Programmed remodeling of cell surface glycans by the sequential action of specific glycosyltransferases, can control biological processes by generating or masking ligands for endogenous lectins. Galectins, a family of animal lectins with affinity for β-galactosides, can form multivalent complexes with cell surface glycoconjugates and deliver a variety of intracellular signals to modulate cell activation, differentiation, and survival. Recent efforts involving genetic or biochemical manipulation of O-and N-glycosylation pathways, as well as blockade of the synthesis of endogenous galectins, have illuminated essential roles for galectin-glycoprotein lattices in the control of biological processes including receptor turnover and endocytosis, host-pathogen interactions and immune cell activation and homeostasis.

show abstract

“…2, C and D). The presence of sialic acid rules out an alternative biantennary N-glycan terminated by two ␣1,3-galactoses, a structure previously described in T cell blasts (34).…”

Section: Maldi-tof Mass Spectrometry and Hpaec Analysis Of N-glycan Smentioning

confidence: 99%