Activation of indole-3-acetic acid oxidase from horseradish and Prunus by phenols and H2O2

Abstract

The enzyme-catalysed oxidation of indole-3-acetic acid (IAA) was systematically investigated with respect to enzyme source and cofactor influence using differential spectrophotometry and oxygen uptake measurement . Commercially-available horseradish peroxidase (HRP) and a peroxidase preparation from Prunus phloem showed identical catalytic properties in degrading IAA . There was no lag phase of IAA oxidation with any of the reaction mixtures tested . Monophenols exhibited a much stronger stimulatory effect than inorganic cofactors, but during the incubation of IAA the phenols were also gradually oxidised. Hydrogen peroxide (H,0,) in combination with monophenols accelerated peroxidation of the monophenol and IAA oxidation simultaneously . Since photometric determination of IAA was affected by oxidation products of dichlorophenol or phenol contamination of the enzyme preparation used, the standard IAA absorption measurements appear to be susceptible to methodological errors . Under certain incubation conditions a catalase-like activity of HRP during the course of IAA oxidation was noted and substrate inhibition was observed above 1 .5 X 10 -4 M IAA . Some concepts concerning the mode of activation of the enzyme-catalysed IAA oxidation are deduced from the experimental results .

Peroxidases in Acetabularia: their possible role in development

“…Moreover, evidence for two IAA degradation pathways has recently been presented [15]. Finally, compound I (an enzyme intermediate with a higher oxidation number than peroxidase) may be involved in IAA oxidation [13]. It should be borne in mind, however, that some basic peroxidase activity might have escaped detection if it were complexed with an inhibitor.…”

Influence of boron deficiency on 3H indole-3yl-acetic acid uptake and efflux in cell cultures of Daucus carota L.

“…It has been suggested that one of the early effects of boron (B) deficiency in plant tissues is an alteration in auxin concentration [4], although several authors [7, 12,] have demonstrated that there is no direct influence on auxin levels . Our unpublished observations on IAA and ABA concentrations in root and shoot tips indicated that phytohormone translocation might be affected under low B supply .…”