Influence of boron deficiency on 3H indole-3yl-acetic acid uptake and efflux in cell cultures of Daucus carota L.

Goldbach, Heiner E.; Amberger, A.

doi:10.1007/bf00025352

Cited by 7 publications

(2 citation statements)

References 17 publications

(17 reference statements)

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“…Besides boron's influence on peroxidase/IAA-oxidase activities (5, 6), a similar interaction between auxins and B has been found for the rooting of mung bean cuttings (8). B may either affect the cellular distribution of auxins (6) or even somehow regulate their action. The effects of B deficiency on Em and proton release are in agreement with earlier observations ofa reduced phosphate efflux (4).…”

Section: Resultsmentioning

confidence: 68%

Influence of Boron on the Membrane Potential in Elodea densa and Helianthus annuus Roots and H⁺ Extrusion of Suspension Cultured Daucus carota Cells

Blaser-Grill¹,

Knoppik²,

Amberger³

et al. 1989

Plant Physiol.

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When following the membrane potential of Elodea densa leaf cells during a dark-light regime and analysing the different phases of the cycle, the pattern under boron deficiency resembled the one reported to occur after 3-(3,4-dichlorophenyl)-1,1-dimethylurea application. The potential in the dark slowly decreased (1,12,14,17,18,20). It is still a matter of controversy whether these differences in net uptake are the consequence of a reduced active transport system such as H+-pumping ATPases (14) or of a more or less general effect on membrane structure (13). The latter assumption is supported by the fact that in several cases of B deficiency lower flux rates for the ions K+, Rb+, and H2P04-were found (4,17,18,20), although a higher net efflux of K+ (23) ary events of B deficiency and lead to a better understanding of boron's role in plant metabolism. MATERIALS AND METHODS Plant Cultivation and Nutrient SolutionsElodea densa was grown in a 0.1 strength nutrient solution according to Hoagland and Arnon (7). The B supply as B(OH)3 was modified as stated in the tables and figures. Helianthus annus was raised hydroponically in a 0.2 N Hoagland solution (see above) for about 6 to 8 weeks after germination. Suspension-cultured cells ofDaucus carota were raised as described earlier (4). The B-deficiency treatments were started either by changing the solution during the potential measurements or by preincubating plant parts (Elodea), whole plants (Helianthus), or thoroughly washed cells (Daucus) in an essentially B-free solution. The nutrient solution employed for the experiments was either a 0.2 strength Hoagland solution (Elodea, Helianthus) or a modified Murashige and Skoog medium (without Fe for ferricyanide reduction to avoid the formation of blue-colored cyanide-complexes). For continuously monitoring HW-release of Daucus cells, a simplified solution, containing only 3% (w/v) saccharose, 0.1 mM CaSO4, and 10 mM K2SO4 was used. To minimize B contamination, highest purity grade chemicals and ultrapure water (Seradest Up) were used, and the storage of solution in glassware and low quality plastics was avoided throughout. If required, solutions were treated additionally with the boratespecific Amberlite IRA 743 ion exchanger (analytical grade, prewashed in ultrapure water).K was determined by flame photometry (Elex 6361, Eppendorf) after wet ashing in HNO3/HCl04/H2SO4 (20:2.5:1, v/ v).

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Section: Resultsmentioning

confidence: 68%

Influence of Boron on the Membrane Potential in Elodea densa and Helianthus annuus Roots and H⁺ Extrusion of Suspension Cultured Daucus carota Cells

Blaser-Grill¹,

Knoppik²,

Amberger³

et al. 1989

Plant Physiol.

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“…Boron has been associated with the maintenance of cell division and cell enlargement (Josten and Kutschera, 1999) and lignin biosynthesis (George and De Klerk, 2008). It also has been suggested as a structural component of primary cell walls (Hu et al, 1996) and to have a role in the control of endogenous auxin levels (Jarvis and Booth, 1981) by promoting IAA destruction and translocation (Goldbach and Amberger, 1986;Jarvis, 1984). Zinc is required for the synthesis of the auxin precursor tryptophan (Trp) (Blazich, 1988;Marschner, 1995), and is also a structural component of auxin receptor ABP1 (Auxin-Binding Protein 1; Tromas et al, (2010)).…”

Section: Other Factors Known To Affect Adventitious Rootingmentioning

confidence: 99%

Reviewing current knowledge on olive (Olea europaea L.) adventitious root formation

Porfírio

Silva

Cabrita

et al. 2016

Scientia Horticulturae

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Olive (Olea europaea) is one of the most important fruit species in the Mediterranean basin, where 95% of the world's olive orchards are planted, and it has become an economically valuable crop worldwide, due to an increasing interest in olive oil for human consumption. New olive orchards are being planted outside the Mediterranean, calling for an effort to identify the genotypes best adapted to the new conditions. However, some olive cultivars remain difficult to propagate, which significantly reduces the capacity to use the full genetic diversity of the species. Improving rooting ability in cuttings from recalcitrant olive cultivars has become a critical topic, which implies fundamental research on the anatomy, physiology, biochemistry and genetics of the adventitious root formation process. Besides, the existence of different rooting behaviors among olive cultivars also makes the species a candidate model plant for these studies. Olive propagation techniques evolved through time from field-or nursery-planted hardwood cuttings, to semi-hardwood cuttings in greenhouses under mist, and, more recently, to in vitro culture techniques. Nevertheless, research about adventitious root formation carried on each propagation method was mostly based on trial and error approaches. Researchers have mainly investigated different factors involved in the process of adventitious rooting by testing their effect in the rooting capacity of different cultivars, leading to a high dispersion and fragmentation of the available information. The goal of this review is to present the most relevant results achieved on adventitious root formation in olive cuttings, aiming to provide an integrated perspective of the current knowledge.

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The Components of Plant Tissue Culture Media I: Macro- and Micro-Nutrients

George¹,

Hall

Klerk³

Plant Propagation by Tissue Culture

136

140

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Influence of boron deficiency on 3H indole-3yl-acetic acid uptake and efflux in cell cultures of Daucus carota L.

Cited by 7 publications

References 17 publications

Influence of Boron on the Membrane Potential in Elodea densa and Helianthus annuus Roots and H⁺ Extrusion of Suspension Cultured Daucus carota Cells

Influence of Boron on the Membrane Potential in Elodea densa and Helianthus annuus Roots and H⁺ Extrusion of Suspension Cultured Daucus carota Cells

Reviewing current knowledge on olive (Olea europaea L.) adventitious root formation

The Components of Plant Tissue Culture Media I: Macro- and Micro-Nutrients

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Influence of boron deficiency on 3H indole-3yl-acetic acid uptake and efflux in cell cultures of Daucus carota L.

Cited by 7 publications

References 17 publications

Influence of Boron on the Membrane Potential in Elodea densa and Helianthus annuus Roots and H+ Extrusion of Suspension Cultured Daucus carota Cells

Influence of Boron on the Membrane Potential in Elodea densa and Helianthus annuus Roots and H+ Extrusion of Suspension Cultured Daucus carota Cells

Reviewing current knowledge on olive (Olea europaea L.) adventitious root formation

The Components of Plant Tissue Culture Media I: Macro- and Micro-Nutrients

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Product

Resources

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Influence of Boron on the Membrane Potential in Elodea densa and Helianthus annuus Roots and H⁺ Extrusion of Suspension Cultured Daucus carota Cells

Influence of Boron on the Membrane Potential in Elodea densa and Helianthus annuus Roots and H⁺ Extrusion of Suspension Cultured Daucus carota Cells