Activation of hyaluronidase by metallic salts and compound 48/80, and inhibitory effect of anti-allergic agents on hyaluronidase.

Kakegawa, Hisao; Matsumoto, Hitoshi; Satoh, Toshifumi

doi:10.1248/cpb.33.642

Cited by 85 publications

(76 citation statements)

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“…Previous studies have reported that patients with burns, septicemia, or shock have rapid increases in circulating hyaluronic acid levels because of the activation of hyaluronidase (Engstrom-Laurent & Hellstrom, 1990;Ferrara et al, 1991;Onarheim et al, 1991a,b). The hyaluronidase inhibition has been demonstrated in other anti-inflammatory drugs such as salicylates (Guerra, 1946) and tranilast (Kakegawa et al, 1985). S. scilloides may contribute to the suppression of hyaluronidase-related dermal burns and possibly other inflammation-related disorders.…”

Section: Lipoxygenase and Hyaluronidase Inhibitions By S Scilloides mentioning

confidence: 97%

“…Based on the detection of liberated N-acetyl glucosamine end group, the enzymatic activity as well as inhibition rate was determined by the Morgan-Elson method (Reissig et al, 1955) as modified (Aronson & Davidson, 1967;Kakegawa et al, 1985). Hyaluronidase, compound 48/80, and hyaluronic acid potassium salt were first prepared in 0.1 M acetate buffer (pH 4.0).…”

Section: Lipoxygenase Inhibitionmentioning

confidence: 99%

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Inhibitory effects of the ethyl acetate extract from bulbs ofScilla scilloideson lipoxygenase and hyaluronidase activities

Nishida

Sugahara

Wada

et al. 2014

Pharmaceutical Biology

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Context: Scilla scilloides Druce (Liliaceae) is a folk medicine to treat dermal inflammation; however, the medicinal properties of this plant have not been completely established. Objective: The current study investigates the potent anti-inflammatory effects of S. scilloides bulbs for its traditional usage using lipoxygenase and hyaluronidase as the inflammation model. To gain insight into the active constituents, nine homoisoflavones (1-9) were subsequently tested. Materials and methods: Lipoxygenase and hyaluronidase inhibition of ethyl acetate extract from the bulbs of this plant within 2000 mg/mL or homoisoflavones within 1000 mM were determined by colorimetric methods. RAW264.7 cells were incubated with 10 or 50 mM homoisoflavones plus lipopolysaccharide (LPS) for 24 h. The culture media were collected and analyzed for determination of the nitric oxide (NO) level by the colorimetric Griess method to measure the extent of inflammation. Results: The extract exhibited inhibitory effects on lipoxygenase and hyaluronidase activities with IC 50 values 31.5 and 169 mg/mL, respectively. Among the nine homoisoflavones tested, four (1 and 3-5) resulted in 79.3-97.9% higher lipoxygenase inhibition than 6.7-32.7% of the others at 500 mM. Calculated IC 50 values indicated 5 as the compound responsible for strong lipoxygenase inhibition with 15.8 mM as the IC 50 value. In the hyaluronidase assay, all homoisoflavones tested at 1000 mM demonstrated 16.2-58.0% inhibition. Incubating the cells in the presence of all nine homoisoflavones tested at 50 mM significantly suppressed the NO production, downward to 1.5-66.0%, in the LPS-activated macrophage cells as a model. Discussion and conclusion: These results may indicate a potential role of S. scilloides for anti-inflammatory purposes.

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Section: Lipoxygenase and Hyaluronidase Inhibitions By S Scilloides mentioning

confidence: 97%

Section: Lipoxygenase Inhibitionmentioning

confidence: 99%

Inhibitory effects of the ethyl acetate extract from bulbs ofScilla scilloideson lipoxygenase and hyaluronidase activities

Nishida

Sugahara

Wada

et al. 2014

Pharmaceutical Biology

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“…Anti-inflammatory agents such as disodium cromoglycate (DSCG) are known to inhibit hyaluronidase activity. DSCG showed stronger inhibitory effect on hyaluronidase when it was added to the enzyme solution prior to a metal salt (Kakegawa et al , 1985). There was approximately twenty times difference of IC 50 values between them.…”

Section: Discussionmentioning

confidence: 96%

“…The enzyme was reported to play a key role in the development of inflammatory diseases, since the destruction of the connective extracellular matrix promotes the spreading of chemotactic factors of inflammation (Facino et al, 1993). In addition, a considerable amount of hyaluronidase is present in rat peritoneal mast cells, and it has been suggested that hyaluronidase is one of the enzymes directly controlling the degranulation of mast cells (Kakegawa et al, 1985). Hyaluronidase has also been reported to be implicated in tumor invasiveness and metastasis.…”

mentioning

confidence: 99%

Hyaluronidase-Inhibiting Polysaccharide Isolated and Purified from Hot Water Extract of Sporophyll of Undaria pinnatifida

Katšube

Yamasaki

Iwamoto

et al. 2003

FSTR

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Polysaccharide showing inhibitory activity against hyaluronidase, which is known to be related to inflammation and tumor metastasis, was purified from the sporophyll of Undaria pinnatifida by fractionated extraction and column chromatography. On the basis of chemical analyses, the purified compound was found to be a kind of sulfated polysaccharide. The molar ratio of sugars and sulfuric acid in the purified compound was estimated to be L-fucose : D-galactose : D-glucuronic acids : sulfuric acid‫0.1؍‬ : 1.0 : 0.04 : 5.2. The number-average molecular weight of the sulfated polysaccharide was estimated to be 63,000 by high-performance liquid chromatography. This polysaccharide inhibited hyaluronidase activity (IC 50 ‫0.31؍‬ g/ml) in a dose-dependent manner.

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“…These agents have shown stronger inhibition against the activation of hyaluronidase by metal salts and compound 48/80 than activated hyaluronidase. 8) The inhibitory effects against the activation of inactive hyaluronidase and activated hyaluronidase were measured. As a result, all three types of alginate with a molecular weight of 32 kDa inhibited both the activation of inactive hyaluronidase (Fig.…”

mentioning

confidence: 99%

Inhibitory Effect of Alginic Acids on Hyaluronidase and on Histamine Release from Mast Cells

Asada

Sugie

Inoue

et al. 1997

Bioscience, Biotechnology, and Biochemistry

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Activation of hyaluronidase by metallic salts and compound 48/80, and inhibitory effect of anti-allergic agents on hyaluronidase.

Cited by 85 publications

References 0 publications

Inhibitory effects of the ethyl acetate extract from bulbs ofScilla scilloideson lipoxygenase and hyaluronidase activities

Inhibitory effects of the ethyl acetate extract from bulbs ofScilla scilloideson lipoxygenase and hyaluronidase activities

Hyaluronidase-Inhibiting Polysaccharide Isolated and Purified from Hot Water Extract of Sporophyll of Undaria pinnatifida

Inhibitory Effect of Alginic Acids on Hyaluronidase and on Histamine Release from Mast Cells

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