Activation of estrogen receptor β reduces blood–brain barrier breakdown following ischemic injury

Shin, Jeong-Ah; Yang, Shuo; Jeong, Sae Im; Park, H.J.; Choi, Youn Hee; Park, E.-M.

doi:10.1016/j.neuroscience.2013.01.031

Cited by 36 publications

(22 citation statements)

References 49 publications

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“…Recruitment of myelin-reactive encephalitogenic T-cells into the CNS (and myelin destruction) are hallmarks of EAE (Anderton 2010), one potential mechanism of estrogen-mediated suppression of CNS inflammation might be inhibition of the BBB breakdown by immune cells (Liu et al 2005;Shin et al 2013), resulting in reduced infiltration into the CNS. Given the crucial role of T H 17 cells in development of EAE, suppression of their infiltration into the CNS is likely an important mechanism underlying any beneficial effect of estrogen on this disease (ElBehi et al 2011).…”

Section: Discussionmentioning

confidence: 99%

Medium-dose estrogen ameliorates experimental autoimmune encephalomyelitis in ovariectomized mice

Haghmorad

Salehipour

Nosratabadi

et al. 2016

Journal of Immunotoxicology

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Estrogen is a neuro-protective hormone in various central nervous system (CNS) disorders. The present study evaluated the role of estrogen during experimental autoimmune encephalomyelitis (EAE) at doses selected to mimic any suppressive potential from the hormone during pregnancy. Here, mice were ovariectomized and then 2 weeks later treated with MOG antigen to induce EAE. Concurrently, mice then received (subcutaneously) an implanted pellet to deliver varying estrogen amounts over a 21-day period. Clinical scores and other parameters were monitored daily for the 21 days. At the end of the period, brain/spinal cord histology was performed to measure lymphocyte infiltration; T-cell profiles were determined through ELISA, flow cytometry, and real-time PCR. Transcription factor expression levels in the CNS were assessed using real-time PCR; T-cell differentiation was evaluated via flow cytometry. The results demonstrated that estrogen inhibited development of EAE. Histological studies revealed limited leukocyte infiltration into the CNS. High and medium dose of estrogen increased T H 2 and T reg cell production of interleukin (IL)-4, IL-10, and transforming growth factor (TGF)-b, but concurrently resulted in a significant reduction in production of interferon (IFN)-c, IL-17, and IL-6. Flow cytometry revealed there were also significant decreases in the percentages of T H 1 and T H 17 cells, as well as significant increase in percentages of T reg and T H 2 cells in the spleen and lymph nodes. Real-time PCR results indicated that high-and medium-dose estrogen treatments reduced T-bet and ROR-ct factor expression, but enhanced Foxp3 and GATA3 expression. Collectively, these results demonstrated that a medium dose of estrogen -similar to a pregnancy level of estrogen -could potentially reduce the incidence and severity of autoimmune EAE and possibly other autoimmune pathologies.ARTICLE HISTORY

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Section: Discussionmentioning

confidence: 99%

Medium-dose estrogen ameliorates experimental autoimmune encephalomyelitis in ovariectomized mice

Haghmorad

Salehipour

Nosratabadi

et al. 2016

Journal of Immunotoxicology

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“…Procedures for transient MCAO and cerebral blood flow (CBF) monitoring were previously described (Shin et al, 2013). After 30 min MCAO, only the mice that exhibited a greater than 85% reduction in CBF during MCAO and recovered by more than 80% after 10 min of reperfusion were included.…”

Section: Transient Middle Cerebral Artery Occlusion (Mcao)mentioning

confidence: 99%

Visceral adipose tissue inflammation is associated with age-related brain changes and ischemic brain damage in aged mice

Shin

Jeong

Kim

et al. 2015

Brain, Behavior, and Immunity

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“…A previous study showed that ER-β activation significantly reduced the protein levels of VEGF and its transcription factor HIF-1α in brain tissues and reduced BBB breakdown [12]. Thus, we examined the regulatory effects of Buk music on ER-β mRNA levels in the brain.…”

Section: Resultsmentioning

confidence: 99%

“…A number of studies have reported important roles for ER-β in affective behaviors, cognitive functions, and neurogenesis [12,31,32]. ER-β activation contributes to the down-regulation of HIF-1α and VEGF in the brain [12].…”

Section: Discussionmentioning

confidence: 99%

“…Estrogen has potent neuroprotective effects in animal models of brain disease or injury via various mechanisms, which include anti-inflammation, reduction in oxidative stress and excitotoxicity, anti-apoptosis, and vasodilation in the ischemic brain [11], and it has also been reported that estrogen receptor-β (ER-β) activation in the brain is associated with reduced blood-brain barrier (BBB) breakdown via the inhibition of HIF-1α/VEGF signaling [12]. …”

Section: Introductionmentioning

confidence: 99%

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The Sound of a Buk (Korean Traditional Drum) Attenuates Anaphylactic Reactions by the Activation of Estrogen Receptor-β

Kim

Nam

et al. 2015

Int Arch Allergy Immunol

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Background: Anaphylaxis is associated with systemic vasodilation that causes low blood pressure and induces hypoxic brain damage. The sound of a Buk (Korean traditional drum) is similar to the human heart beat and affects blood pressure, heart rate, and the nervous system by increasing physiological excitation and sympathetic nervous system activity. So, this study focused on the effect of Buk music as a means of treating anaphylaxis. Methods: Mice were given an intraperitoneal injection of compound 48/80 (6.5 mg/kg, a mast cell degranulator). After compound 48/80 injection, mice were exposed to Buk music and white noise for 5 min in a sound isolation booth. The mortality rate was checked over the next 40 min. Levels of hypoxia-inducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF) in the serum and brain tissues were analyzed by Western blotting, quantitative real-time PCR, and ELISA methods. Results: Exposure to Buk music significantly reduced compound 48/80-induced mortality and histamine release, as well as HIF-1α and VEGF levels compared with the compound 48/80 group or compound 48/80 and white noise group. Buk music also reduced levels of tumor necrosis factor-α, and significantly increased estrogen receptor-β mRNA levels. Conclusion: These results indicate that Buk music has potential for the treatment of anaphylaxis.

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Activation of estrogen receptor β reduces blood–brain barrier breakdown following ischemic injury

Cited by 36 publications

References 49 publications

Medium-dose estrogen ameliorates experimental autoimmune encephalomyelitis in ovariectomized mice

Medium-dose estrogen ameliorates experimental autoimmune encephalomyelitis in ovariectomized mice

Visceral adipose tissue inflammation is associated with age-related brain changes and ischemic brain damage in aged mice

The Sound of a Buk (Korean Traditional Drum) Attenuates Anaphylactic Reactions by the Activation of Estrogen Receptor-β

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