Activation of connective tissue-type and mucosal-type mast cells in compound 48/80-induced airway response

Liu, Shuang; Hiedayati, Nurul; Shudou, Masachika; Maeyama, Kazutaka

doi:10.1016/j.ejphar.2005.10.067

Cited by 7 publications

(10 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In ovalbumin-sensitised rats, the number of MMCs increases before the migration of other inflammatory cells into the airways during the early or late phases of the asthmatic response on immunological or non-immunological challenge. 6,9 The difference between the previous studies and the present study is that we observed an increase in MMCs even before the occurrence of the allergic asthmatic processes. In addition, the activation of MMCs occurred before any increases in the IgE level, airway hypersensitivity or infiltration of other inflammatory cells were observed.…”

Section: Discussioncontrasting

confidence: 97%

“…By contrast, the granules of MMCs contain chondroitin sulphate di‐B proteoglycans and RMCPII, but they have substantially less histamine than CTMCs. Previous studies have shown that different types of mast cell can exist in various activation states during both the early and the late phases of the asthmatic response 6 , 9 . In this study, activation of MMCs occurred in various ways on primary damage of airway defences during allergic asthmatic processes, whereas activation of CTMCs occurred in parallel to the appearance of other typical asthmatic features.…”

Section: Discussionmentioning

confidence: 49%

“…Previous studies have shown that different types of mast cell can exist in various activation states during both the early and the late phases of the asthmatic response. 6,9 In this study, activation of MMCs occurred in various ways on primary damage of airway defences during allergic asthmatic processes, whereas activation of CTMCs occurred in parallel to the appearance of other typical asthmatic features. In humans, two potentially analogous mast cell subsets, referred to as MC T and MC TC , have been identified based on their granule protease content and their tissue localisation.…”

Section: Discussionmentioning

confidence: 61%

“…Mast cells were identified after staining with Alcian blue and safranin and were categorised into two groups based on their staining patterns; at pH 1.0, MMCs stain light blue, whereas CTMCs stain dark blue or brown 6 . The number of MMCs in the BALF of ovalbumin‐sensitised neonatal rats increased early in the second week of sensitisation and this increase persisted for 4 weeks.…”

Section: Resultsmentioning

confidence: 99%

See 3 more Smart Citations

Early activation of mucosal mast cells during the primary immune response in a rodent model of neonatal asthma

2010

Self Cite

View full text Add to dashboard Cite

During an allergic inflammatory response in the airway, if a failure of the epithelial cell barrier occurs before the systemic immune response is triggered by allergens, more allergens can invade. Using a rat model of asthma, we previously found that mucosal mast cells, which localise to the epithelial layer of the airways, are activated to promote a pro-asthmatic immune response. In this study, we developed a neonatal rat model of allergic airway hypersensitivity that mimics some features of childhood asthma. Airway hypersensitivity was measured using unrestrained whole-body plethysmography after analysis of the serum IgE titre. Inflammatory cells and inflammatory mediators in bronchoalveolar lavage fluid samples were examined. Two mast cell-specific proteases were detected using PCR. In addition, we analysed the phenotype and the number of mast cells in the airways by immunohistochemistry, and we found that the number of mucosal mast cells and the expression level of the proteases increased 2 weeks after sensitisation. Changes in the IgE titre, airway hypersensitivity and the activation of other inflammatory cells were delayed, appearing during the 4 weeks after sensitisation. Our results indicate that the activation of mucosal mast cells contributes to the pro-asthmatic immune response. This activation may be a biomarker allowing early intervention that could help prevent allergic airway inflammation.

show abstract

Section: Discussioncontrasting

confidence: 97%

Section: Discussionmentioning

confidence: 49%

Section: Discussionmentioning

confidence: 61%

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Early activation of mucosal mast cells during the primary immune response in a rodent model of neonatal asthma

2010

Self Cite

View full text Add to dashboard Cite

show abstract

“…Non‐immunological activation of mast cells has been implicated in the inflammatory process observed in asthmatic patients in response to exercise, cold air or hypertonic saline inhalation ( Carroll et al ., 2002 ). Recently, Liu et al . (2006) have described a model of non‐immunological airway responses in rats using compound 48/80, where early and late‐phase responses up to 8 h after treatment were observed by utilizing plethysmography.…”

Section: Introductionmentioning

confidence: 99%

In vivo pharmacological evaluation of compound 48/80‐induced airways oedema by MRI

Karmouty‐Quintana

Blé

Cannet

et al. 2008

British J Pharmacology

View full text Add to dashboard Cite

Background and purpose: Allergen-induced airways oedema in actively sensitized rats has been studied earlier by magnetic resonance imaging (MRI). We used MRI to follow the consequences of non-immunological mast cell activation induced by compound 48/80 in the rat lungs in vivo. Experimental approach: Male naïve rats were scanned by MRI prior to and at several time points following intratracheal administration of the mast cell secretagogue, compound 48/80. The effects of a range of drugs on the response induced by compound 48/80 were studied. Key results: Strong fluid signals were detected by MRI in the lungs at 24 h after compound 48/80, correlating with increased protein concentration and inflammatory cell infiltration in bronchoalveolar lavage, and with perivascular oedema observed histologically. Pharmacological intervention demonstrated that the increase in MRI signal volume induced by compound 48/80 24 h after challenge was blocked by disodium cromoglycate and the glucocorticoid, budesonide. Pretreatment with wortmannin, capsazepine, DNK333 (a dual neurokinin (NK) 1 and NK2 antagonist) or the anti-allergy drug CGS8515, but not indomethacin, resulted in partial inhibition. Conclusions and implications:Compound 48/80 induced a complex inflammatory reaction which did not solely involve mast cell degranulation but also activation of sensory nerves and was qualitatively similar to allergen challenge. Changes observed by MRI correlated with decreases in protein concentration in BAL fluid. However, the magnitude of the changes detected was greater using MRI. Our results demonstrate that MRI is a sensitive and efficient tool to assess the effects of drugs on lung inflammation.

show abstract

A novel MRGPRX2-targeting antagonistic DNA aptamer inhibits histamine release and prevents mast cell-mediated anaphylaxis

Suzuki

Liu

Ogasawara

et al. 2020

European Journal of Pharmacology

View full text Add to dashboard Cite

Activation of connective tissue-type and mucosal-type mast cells in compound 48/80-induced airway response

Cited by 7 publications

References 31 publications

Early activation of mucosal mast cells during the primary immune response in a rodent model of neonatal asthma

Early activation of mucosal mast cells during the primary immune response in a rodent model of neonatal asthma

In vivo pharmacological evaluation of compound 48/80‐induced airways oedema by MRI

A novel MRGPRX2-targeting antagonistic DNA aptamer inhibits histamine release and prevents mast cell-mediated anaphylaxis

Contact Info

Product

Resources

About