Activation of co-insensitive respiration in echiuroid eggs by light irradiation at various wavelengths

Observation of In Vivo Cytochrome‐Based Electron‐Transport Dynamics Using Time‐Resolved Evanescent Wave Electroabsorption Spectroscopy

“…This dissociation generates redox‐active c ‐Cyts and revives the cellular respiratory electron‐transport reactions (Figure 1 a). 2f Thus, our findings demonstrate the successful artificial control of the in vivo electron‐transport chain of bacterial metabolism by the photochemistry of CO‐ligated c ‐Cyts (Figure S3 in the Supporting Information).…”

“…To accomplish this observation, the following techniques were employed: 1) For direct investigation of in vivo respiratory electron‐transport dynamics of bacteria, we applied the artificial photochemical reaction of CO to the bacterium S. loihica PV‐4 (Figure 1 a). In this system, respiration is inhibited by blockage of the redox activities of hemes upon CO binding but can be subsequently reactivated by photodissociation of CO 2f. 2) To distinguish between single photochemical reaction dynamics and in vivo respiratory electron‐transport dynamics, secondary artificial control between active and inactive electron‐transport chains should be appended to the analysis, in addition to the photochemical control.…”