Activation of cannabinoid receptors promote periodontal cell adhesion and migration

Liu, Chunyan; Qi, Xia; Alhabeil, Jamal A.; Lu, Haiyan; Zhou, Zheng

doi:10.1111/jcpe.13190

Cited by 28 publications

(27 citation statements)

References 43 publications

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“…It was shown that AEA is able to control and restrict immune responses after primary CNS damage over a cannabinoid receptormediated pathway, and investigations further evidenced that AEA driven effects in microglia are mainly induced through CB2 receptors [23,42]. Recent works on PDL cells already found an impact of CB2 on cell adhesion and migration [9]. In the present study, CB receptor regulation in microglia was unaffected by stimulation, indicating a stable expression not being modulated by external influences.…”

Section: Discussionsupporting

confidence: 67%

“…Our previous investigations identified coexpression of cannabinoid receptors CB1 and CB2 on PDL cells, as it was seen for peripheral immune cells as well, potentially qualifying them as an important target for cannabinoid-driven regulation of periodontal immunology [8]. In addition, it was found that cannabinoids are able to promote periodontal cell adhesion and migration and thus induce cellular wound healing and regeneration processes [9]. Furthermore, CB receptor activation can facilitate osteogenic differentiation of PDL cells by upregulation of corresponding gene expression patterns and induction of mineralization processes, and presumably also in an inflammatory setting [10,11].…”

Section: Introductionmentioning

confidence: 82%

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Analogous modulation of inflammatory responses by the endocannabinoid system in periodontal ligament cells and microglia

et al. 2020

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Background Periodontal ligament (PDL) cells initiate local immune responses, similar to microglia regulating primary host defense mechanisms in neuroinflammatory events of the central nervous system. As these two cell types manifest similarities in their immunomodulatory behavior, this study investigated the thesis that the immunological features of PDL cells might be modulated by the endocannabinoid system, as seen for microglia. Methods A human PDL cell line and an Embryonic stem cell-derived microglia (ESdM) cell line were grown in n = 6 experimental groups each, incubated with cannabinoid receptor agonists arachidonoylethanolamine (AEA) (50 μM) or Palmitoylethanolamide (PEA) (50 μM) and challenged with centrifugation-induced inflammation (CII) for 6 and 10 h. Untreated samples served as controls. Quantitative real-time polymerase chain reaction was applied for gene expression analyses of inflammatory cytokines, cannabinoid receptors and ionized calcium binding adaptor molecule 1 (IBA-1). Microglia marker gene IBA-1 was additionally verified on protein level in PDL cells via immunocytochemistry. Proliferation was determined with a colorimetric assay (WST-1 based). Statistical significance was set at p < 0.05. Results IBA-1 was inherently expressed in PDL cells both at the transcriptional and protein level. AEA counteracted pathological changes in cell morphology of PDL cells and microglia caused by CII, and PEA contrarily enhanced them. On transcriptional level, AEA significantly downregulated inflammation in CII specimens more than 100-fold, while PEA accessorily upregulated them. CII reduced cell proliferation in a time-dependent manner, synergistically reinforced by PEA decreasing cell numbers to 0.05-fold in PDL cells and 0.025-fold in microglia compared to controls. Conclusion PDL cells and microglia exhibit similar features in CII with host-protective effects for AEA through dampening inflammation and preserving cellular integrity. In both cell types, PEA exacerbated proinflammatory effects. Thus, the endocannabinoid system might be a promising target in the regulation of periodontal host response.

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Section: Discussionsupporting

confidence: 67%

Section: Introductionmentioning

confidence: 82%

Analogous modulation of inflammatory responses by the endocannabinoid system in periodontal ligament cells and microglia

et al. 2020

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“…Both CB1 and CB2 receptors are found in periodontal tissues, and their expression may change under different conditions (Konermann, Jager, Held, Brossart, & Schmole, 2017;Liu, Qi, Alhabeil, Lu, & Zhou, 2019). In healthy periodontal tissues, CB1 receptors are expressed at a significantly higher level than CB2 receptors in periodontal ligament (PDL).…”

Section: Periodontal Tissuementioning

confidence: 99%

The effects of cannabis use on oral health

Liu

Yang

et al. 2019

Oral Diseases

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Cannabis, also known as marijuana, is one of the most commonly used substances for medical and recreational purposes globally. With the trend of global legalization of medical use of cannabis and even the recreational use, the prevalence of recreational use of cannabis has increased markedly over the past few years. Correspondingly, the potential health concerns related to cannabis consumption have also increased. Therefore, it is necessary for oral healthcare providers to understand the effects of cannabis use on oral health. This review briefly summarizes the components of cannabis, biologic activities on tissues, and mechanisms of action in human cells and tissues. Oral tissue expression of cannabinoid receptors and the potential association of cannabis to oral diseases are also examined. The goals of this review are to (1) elaborate the basic biology and physiology of cannabis in human oral tissues, and (2) provide a better understanding the effects of its use and abuse on oral health. Due to insufficient information, more well‐designed studies should be conducted. It is urgent to include cannabis usage into dental patient health records.

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“…In periodontal tissues, several reports have suggested a role for both CB 1 and CB 2 receptors in pathological conditions, such as inflammation and wound healing [ 103 , 104 , 105 ]. Indeed, CB 1 are expressed at a significantly higher level than CB 2 receptors in both epithelium and periodontal ligaments (PDL) in periodontal tissues from healthy subjects.…”

Section: Introductionmentioning

confidence: 99%

“…Periodontal tissue cannabinoid receptors have been suggested to differentially regulate cell growth and differentiation, inflammatory processes, and tissue healing [ 104 , 106 , 107 , 108 , 109 , 110 , 111 , 112 , 113 , 114 , 115 ], indicating that distinct expression patterns of CB 1 and CB 2 in PDL may be representative of distinct cellular function [ 104 , 106 , 107 , 108 , 109 ]. For instance, Liu et al showed that cannabinoids, by activating FAK and MAPK signaling in a CB 2 -dependent manner, trigger periodontal cell adhesion and migration [ 104 ], which provides evidence for therapeutic potential of cannabinoid compounds in periodontal regeneration and wound healing, possibly associated with the anti-inflammatory actions of CB 1 receptor activation, via NF-kappaB pathway inhibition in the periodontal tissue, as reported by Nakajima and colleagues [ 109 ].…”

Section: Introductionmentioning

confidence: 99%

Cannabinoids Drugs and Oral Health—From Recreational Side-Effects to Medicinal Purposes: A Systematic Review

Bellocchio

Inchingolo

et al. 2021

IJMS

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Background: marijuana, the common name for cannabis sativa preparations, is one of the most consumed drug all over the world, both at therapeutical and recreational levels. With the legalization of medical uses of cannabis in many countries, and even its recreational use in most of these, the prevalence of marijuana use has markedly risen over the last decade. At the same time, there is also a higher prevalence in the health concerns related to cannabis use and abuse. Thus, it is mandatory for oral healthcare operators to know and deal with the consequences and effects of cannabis use on oral cavity health. This review will briefly summarize the components of cannabis and the endocannabinoid system, as well as the cellular and molecular mechanisms of biological cannabis action in human cells and biologic activities on tissues. We will also look into oropharyngeal tissue expression of cannabinoid receptors, together with a putative association of cannabis to several oral diseases. Therefore, this review will elaborate the basic biology and physiology of cannabinoids in human oral tissues with the aim of providing a better comprehension of the effects of its use and abuse on oral health, in order to include cannabinoid usage into dental patient health records as well as good medicinal practice. Methods: the paper selection was performed by PubMed/Medline and EMBASE electronic databases, and reported according to the PRISMA guidelines. The scientific products were included for qualitative analysis. Results: the paper search screened a total of 276 papers. After the initial screening and the eligibility assessment, a total of 32 articles were considered for the qualitative analysis. Conclusions: today, cannabis consumption has been correlated to a higher risk of gingival and periodontal disease, oral infection and cancer of the oral cavity, while the physico-chemical activity has not been completely clarified. Further investigations are necessary to evaluate a therapeutic efficacy of this class of drugs for the promising treatment of several different diseases of the salivary glands and oral diseases.

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Activation of cannabinoid receptors promote periodontal cell adhesion and migration

Cited by 28 publications

References 43 publications

Analogous modulation of inflammatory responses by the endocannabinoid system in periodontal ligament cells and microglia

Analogous modulation of inflammatory responses by the endocannabinoid system in periodontal ligament cells and microglia

The effects of cannabis use on oral health

Cannabinoids Drugs and Oral Health—From Recreational Side-Effects to Medicinal Purposes: A Systematic Review

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