Activation of bovine and baboon primordial follicles in vitro

Fortune, J.E.; Kito, Seiji; Wandji, Serge-Alain; Sršeň, Vlastimil

doi:10.1016/s0093-691x(97)00416-0

Cited by 86 publications

(56 citation statements)

References 18 publications

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“…However, in our culture conditions, an elevated activation was observed after the addition of IGF/GH to the culture medium. Follicular activation has been obtained in previous studies in goats (11), cows (34,35) and baboons (36), in which the number of primordial follicles was dramatically reduced, with a concomitant increase in the number of developing follicles after in vitro culture of ovarian tissue. Studies have demonstrated that GH stimulates the production of IGF-I and its messenger RNA (mRNA) in porcine granulosa cells and rat ovary (37).…”

Section: Discussionmentioning

confidence: 78%

Interaction between growth differentiation factor 9, insulin-like growth factor I and growth hormone on the in vitro development and survival of goat preantral follicles

Martins

Celestino

Saraiva

et al. 2010

Braz J Med Biol Res

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Section: Discussionmentioning

confidence: 78%

Interaction between growth differentiation factor 9, insulin-like growth factor I and growth hormone on the in vitro development and survival of goat preantral follicles

Martins

Celestino

Saraiva

et al. 2010

Braz J Med Biol Res

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“…That is to say, high concentrations of FSH and EGF and lower concentrations of GDF-9 and bFGF may be beneficial for the development of primary follicles. Studies on growth factors have demonstrated that FSH and EGF are not essential for primordial follicle activation [24][25][26] but are important for latter follicle development [1,14]. On the other hand, GDF-9 and bFGF can promote the initiation of primordial follicle development and stimulate early preantral follicle growth [5,6,8,[27][28][29].…”

Section: Discussionmentioning

confidence: 99%

In vitro culture of sheep lamb ovarian cortical tissue in a sequential culture medium

Peng

Yang

Wang

et al. 2010

J Assist Reprod Genet

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Purpose This study evaluates the effect of a sequential culture system on the follicular development of sheep lamb ovaries, aiming to establish an available in vitro culture system for ovarian culture. Methods Lamb ovarian cortical fragments were cultured on a steel mesh with a nitrocellulose membrane pre-coated by type1 collagen. Several culture media were used for the determinations, specifically, a control medium (α-MEM), a constant medium (control medium supplemented with 75 ng/mL human recombinant EGF, 200 mIU/mL sheep FSH, 100 ng/mL human recombinant GDF-9, and 100 ng/mL human recombinant bFGF), and a sequential medium (control medium supplemented with sequential growth factors added on different days). Ovarian tissues, both fresh and cultured, were processed for histological and apoptotic assays, while spent culture media were processed for hormone assays.Results It was found that the growth of lamb primordial follicles can be initiated during culture in vitro. Compared to the control medium, sequential culture medium significantly increased the percentage of secondary follicles in cultures, while the follicle and oocyte diameters of primary and secondary follicles were also observed to increase in this medium. The constant medium was found to increase the number and diameter of secondary follicles only 18 days after culture. After this same period of time, some normal antral follicles were found in the sequential medium, while a few abnormal antral-like follicles were found in the control medium. Moreover, sequential medium appeared to significantly increase estradiol and inhibin production, especially 10-18 days after culture. The highest percentage of normal follicles and the lowest apoptotic cell rates were observed in the sequential medium, suggesting that a sequential addition style of culture can improve follicle and tissue viability. Conclusions The sequential addition of FSH, EGF, GDF-9, and bFGF can stimulate primordial follicle transmittal into the later development stages, even as far as the antral stage, improve the survival rate of follicles, and maintain follicular viability.

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“…MEM has been used to culture tissues from various species, including bovine (Braw-Tal & Yossefi, 1997) and caprine (Silva et al, 2004) ovarian tissue as well as murine preantral follicles (Zhao et al, 2001). To prevent follicles from atresia, these media mostly include a protein source (like albumin, fetal calf serum or homogeneous serum), antibiotics, antimycotics, transferring (to bind metal ions) and selenium (to bind free radicals), glutamine, pyruvate (energy sources) and occasionally one or more growth factors (like insulin-like growth factor (IGF)-1, epidermal growth factor (EGF), activin-A, growth differentiation factor-9 (GDF-9) and fibroblast growth factor (FGF)-2), hormones (like insulin, follicle stimulating hormone (FSH), growth hormone, thyroid stimulating hormone (TSH) and thyroxine) and/or steroids (like oestradiol, testosterone or androstenedione), sometimes added in a timely manner (for reviews, see Telfer, 1998;Fortune et al, 1999;Van den Hurk et al, 2000;Picton et al, 2003;Smitz et al, 2010). Ascorbic acid is a protective substance that can be added to the culture medium of early-stage follicles.…”

Section: Introductionmentioning

confidence: 99%

Effects of ascorbic acid on in vitro culture of bovine preantral follicles

Andrade

Hurk

Lisboa

et al. 2012

Zygote

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SummaryThe objective of this study was to evaluate the effects of adding ascorbic acid to the media for in vitro culture of cattle ovarian fragments and to determine their effects on growth activation and viability of early-stage follicles. The ovarian cortex was divided into small fragments; one fragment was immediately fixed (control) and the other fragments were cultured in minimum essential medium (MEM) supplemented or not with various doses of ascorbic acid. Ovarian tissue was processed for histology, transmission electron microscopy (TEM) and immunohistochemical demonstration of proliferating cell nuclear antigen (PCNA). Compared with control fragments, the percentage of primordial follicles was reduced (p < 0.05) and the percentage of growing follicles had increased (p < 0.05) in cultured cortical fragments, independent of the tested medium or incubation time. Furthermore, compared with control tissue, culture of ovarian cortex for 8 days reduced the percentages of healthy, viable follicles (p < 0.05), but not when cultures were supplemented with 25, 50 or 100 g/ml of ascorbic acid. Ultrastructural and immunohistochemical analysis of 8 day cultured ovarian cortical fragments, however, showed the integrity and viability of follicles only when fragments were cultured in presence of 50 g/ml of ascorbic acid. In conclusion, this study demonstrated that addition of ascorbic acid to MEM at a concentration of 50 g/ml not only stimulates the activation of 8 day in vitro cultured cattle primordial follicles and subsequent growth of activated follicles, but also safeguards the viability of these early-stage follicles.

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Activation of bovine and baboon primordial follicles in vitro

Cited by 86 publications

References 18 publications

Interaction between growth differentiation factor 9, insulin-like growth factor I and growth hormone on the in vitro development and survival of goat preantral follicles

Interaction between growth differentiation factor 9, insulin-like growth factor I and growth hormone on the in vitro development and survival of goat preantral follicles

In vitro culture of sheep lamb ovarian cortical tissue in a sequential culture medium

Effects of ascorbic acid on in vitro culture of bovine preantral follicles

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