Activation of blood platelets in echinococcosis — CD62P and CD63 expression

Matowicka-Karna, Joanna; Kemona, H; Dymicka-Piekarska, Violetta; Butkiewicz, A

doi:10.1007/s00436-005-0038-2

Cited by 7 publications

(3 citation statements)

References 26 publications

(29 reference statements)

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“…Cytokines are small molecules that function as key immune mediators. As other investigators have described that G. intestinalis is able to negatively modulate the expression of early pro-inflammatory mediators in order to evade the innate immune response (Matowicka-Karna et al, 2009), we decided to analyse the expression of TNF-a. We did not observe differences between infected (Giardia group) and uninfected control mice (Fig.…”

Section: Determination Of Cytokines and Chemokines In Small-intestinamentioning

confidence: 99%

Administration of kefir-fermented milk protects mice against Giardia intestinalis infection

Franco

Golowczyc

Antoni

et al. 2013

Journal of Medical Microbiology

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Giardiasis, caused by the protozoan Giardia intestinalis, is one of the most common intestinal diseases worldwide and constitutes an important problem for the public health systems of various countries. Kefir is a probiotic drink obtained by fermenting milk with 'kefir grains', which consist mainly of bacteria and yeasts that coexist in a complex symbiotic association. In this work, we studied the ability of kefir to protect mice from G. intestinalis infection, and characterized the host immune response to this probiotic in the context of the intestinal infection. Six-to 8-week-old C75BL/6 mice were separated into four groups: controls, kefir mice (receiving 1 : 100 dilution of kefir in drinking water for 14 days), Giardia mice (infected orally with 4¾10 7 trophozoites of G. intestinalis at day 7) and Giardia-kefir mice (kefir-treated G. intestinalis-infected mice), and killed at 2 or 7 days post-infection. Kefir administration was able to significantly reduce the intensity of Giardia infection at 7 days post-infection. An increase in the percentage of CD4 + T cells at 2 days post-infection was observed in the Peyer's patches (PP) of mice belonging to the Giardia group compared with the control and kefir groups, while the percentage of CD4 + T cells in PP in the Giardia-kefir group was similar to that of controls. At 2 days post-infection, a reduction in the percentage of B220-positive major histocompatibility complex class II medium cells in PP was observed in infected mice compared with the other groups. At 7 days post-infection, Giardiainfected mice showed a reduction in RcFce-positive cells compared with the control group, suggesting a downregulation of the inflammatory response. However, the percentages of RcFcepositive cells did not differ from controls in the kefir and Giardia-kefir groups. An increase in IgApositive cells was observed in the lamina propria of the kefir group compared with controls at 2 days post-infection. Interestingly, the diminished number of IgA-positive cells registered in the Giardia group at 7 days post-infection was restored by kefir feeding, although the increase in IgApositive cells was no longer observed in the kefir group at that time. No significant differences in CXCL10 expression were registered between groups, in concordance with the absence of inflammation in small-intestinal tissue. Interestingly, a slight reduction in CCL20 expression was observed in the Giardia group, suggesting that G. intestinalis might downregulate its expression as a way of evading the inflammatory immune response. On the other hand, a trend towards an increase in TNF-a expression was observed in the kefir group, while the Giardia-kefir group showed a significant increase in TNF-a expression. Moreover, kefir-receiving mice (kefir and Giardia-kefir groups) showed an increase in the expression of IFN-c, the most relevant Th1 cytokine, at 2 days post-infection. Our results demonstrate that feeding mice with kefir reduces G. intestinalis infection and promotes the activation of different mechanisms of ...

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Section: Determination Of Cytokines and Chemokines In Small-intestinamentioning

confidence: 99%

Administration of kefir-fermented milk protects mice against Giardia intestinalis infection

Franco

Golowczyc

Antoni

et al. 2013

Journal of Medical Microbiology

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“…Platelets deliver an antiparasitic effect not only by participating in the process of pathogen elimination (5,25). The release of inflammatory mediators, coating ability, lysis, and phagocytosis minimise the parasite's adverse impact on the host organism and reduce parasite counts (11,12). It may be concluded that parasitic infection increases platelet production and enhances the cytotoxic function of thrombocytes.…”

Section: Discussionmentioning

confidence: 99%

Influence of Invasion of Intracellular Parasites on Platelet Response in Dogs Based on Clinical Cases

Snarska

Pomianowski

Krystkiewicz

et al. 2012

Bulletin of the Veterinary Institute in Pulawy

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Blood morphology in dogs infected with intracellular parasites of the genera Babesia and Ehrlichia was examined. The parasites were detected in peripheral blood smears stained by the May-Grünwald-Giemsa method. During the parasitic invasion, a decrease in thrombocyte counts was observed. This was connected with forming platelet aggregates. It was noted that the number of giant platelets simultaneously increased.

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“…Intermediate hosts ingest dog feces by smear infections, and the Echinococcus eggs form larvae, which travel through the blood and form hydatid cysts in various tissues. There are reports that platelet activation is increased in the blood of echinococcosis patients, as demon strated by increased CD62P exposition, elevated concentrations of platelet granule derived bthromboglobulin and PF4, and high amounts of microparticles [198][199][200]. Platelet activation might be mediated either by direct contact with echinococcal surface structures or by binding to IgE or IgG antibodies coating the parasites; activation by the inflammatory reac tion can also be postulated.…”

Section: Worm Infectionsmentioning

confidence: 98%

Platelets as Immune Cells in Infectious Diseases

et al. 2013

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Platelets have been shown to cover a broad range of functions. Besides their role in hemostasis, they have immunological functions and thus participate in the interaction between pathogens and host defense. Platelets have a broad repertoire of receptor molecules that enable them to sense invading pathogens and infection-induced inflammation. Consequently, platelets exert antimicrobial effector mechanisms, but also initiate an intense crosstalk with other arms of the innate and adaptive immunity, including neutrophils, monocytes/macrophages, dendritic cells, B cells and T cells. There is a fragile balance between beneficial antimicrobial effects and detrimental reactions that contribute to the pathogenesis, and many pathogens have developed mechanisms to influence these two outcomes. This review aims to highlight aspects of the interaction strategies between platelets and pathogenic bacteria, viruses, fungi and parasites, in addition to the subsequent networking between platelets and other immune cells, and the relevance of these processes for the pathogenesis of infections.

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Activation of blood platelets in echinococcosis — CD62P and CD63 expression

Cited by 7 publications

References 26 publications

Administration of kefir-fermented milk protects mice against Giardia intestinalis infection

Administration of kefir-fermented milk protects mice against Giardia intestinalis infection

Influence of Invasion of Intracellular Parasites on Platelet Response in Dogs Based on Clinical Cases

Platelets as Immune Cells in Infectious Diseases

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