Activation Antigens during the Proliferative and Secretory Phases of Endometrium and Early-Pregnancy Decidua

Abstract

Background: Clarifying the normal distribution of activation antigens will contribute to database construction studies of monoclonal-antibody-based therapies in endometrial disorders. Methods: In this study, endometrial tissue samples obtained during proliferative and secretory phases and decidual samples of early pregnancies were immunostained by the monoclonal antibodies anti-CD26, anti-CD30, anti-CD70, anti-CD71, and anti-CD98 using the indirect immunoperoxidase method. Results: CD26 is expressed on the glandular epithelium in the endometrium and decidua. Endothelial CD26 is expressed less in the decidua when compared to the endometrium. CD30 is strongly expressed by decidual cells. It is only weakly expressed on endometrial and decidual vessels. Glandular and endothelial CD70 expression is mainly seen in the proliferative phase of the menstrual cycle. Glandular CD71 expression is less in the decidua when compared to the endometrium. Its expression on stromal cells is more in the secretory phase of the menstrual cycle and in early pregnancy deciduae. It is expressed on endometrial vessels but not on decidual vessels. Glandular CD98 is expressed more in the decidua when compared to the endometrium. This antigen exists on endometrial lymphocytes. It is strongly expressed on the endothelium in the endometrium and decidua. Conclusion: It seems that CD26 and CD70 are not involved in the functions of endometrial and decidual stromal cells. CD30 and CD71 are thought to be involved in decidualization. Absence of activation antigens other than CD98 on lymphocytes indicated an antigenic profile for large granular lymphocytes that is different from regular lymphocytes.

The possible biological role of metallothionein in apoptosis

“…The mucous membrane of the entire reproductive tract is infiltrated by immune system cells and activated immune cytotoxic cells are predominant in this infiltrate (NK cells in the endometrium and CTLs in the tubal mucosa) (104). The activity of these infiltrating immune cells (113)(114)(115)(116) is regulated by mucous membrane cells. The coexistence of immune cells and mucous membrane epithelial cells is related to the development of a resistance to immunemediated apoptosis in epithelial cells, especially endometrial cells (117).…”

Proteomic analysis of pregnancy-related proteins from pig uterus endometrium during pregnancy

“…This remodeling process of endometrial tissue is regulated by several factors, such as the ovarian steroids, various cytokines and growth factors, which influence endometrial differentiation and function, pregnancy recognition signaling, uterine receptivity for blastocyst implantation, and conceptus-uterine interactions. Tissue remodeling shares features with the repair of mucosal injury, characterized by a migratory phenotype with specialized cytoskeletal and matrix-receptor reorganizations and specialized matrix-dependent signaling patterns [4-6]. …”

“…Of all the cell adhesion molecules, endothelial markers and integrins have been extensively investigated in the endometrium and deciduas [13], but comparison of the overall protein expression profile in pregnant and non-pregnant endometria has not been adequately explored. To date, the development of a test for endometriosis has mostly concentrated on the levels of cytokines and growth factors involved in inflammation, angioneogenesis and tissues remodeling that are present in the serum, peritoneal fluid, endometrium and endometriotic lesions during pregnancy [6,14-17]. Because changes in the whole protein expression profile occur during pregnancy, proteomic techniques are now being employed to identify proteins expressed during different stages of pregnancy.…”

Expressão do sistema VEGF-A no útero bovino após tratamento com diferentes doses de eCG

“…Alterações morfológicas que ocorrem no endométrio e no miométrio que são estimuladas por hormônios ovarianos dentre outros. Vale ressaltar que o endométrio e seus fluidos têm importância no processo reprodutivo: transporte dos espermatozóides, regulação da função do CL, início da implantação, gestação e parto(UGUR et al, 2006).…”