Activated CD4+ and CDS+ Cells in the Colonic Mucosa of Ulcerative Colitis Patients: Their Relationship to HLA-DR Antigen Expression on the Colonic Epithelium and Serum Soluble CD25 Levels

Sasakawa, Tetsuya; Takizawa, Hajime; Bannai, Hitoshi; Narisawa, Rintaro; Asakura, Hitoshi

doi:10.1159/000201285

Cited by 10 publications

(5 citation statements)

References 14 publications

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“…Several studies have shown elevated CD25 expression in the mucosa of IBD patients (6,34), a nding that is in keeping with the known level of T cell activation that occurs during the disease process. Increased CD25 expression in the systemic circulation has also been shown in CD (5, 6) with elevated soluble CD25 being found in UC (35). In this study, no increase in the expression of CD25 was seen on lymphocytes isolated from the peripheral blood of pouchitis patients compared to controls.…”

Section: Discussionsupporting

confidence: 53%

Altered Expression of the Lymphocyte Activation Markers CD30 and CD27 in Patients with Pouchitis

Forbes

2001

Scandinavian Journal of Gastroenterology

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Thomas PD, Forbes A, Nicholls RJ, Ciclitira PJ. Altered expression of the lymphocyte activation markers CD30 and CD27 in patients with pouchitis. Scand J Gastroenterol 2001;36:258-264. Background: The mechanism underlying the development of ileal pouch in ammation in ulcerative colitis patients (pouchitis) after restorative proctocolectomy is unclear. Persistent systemic T cell activation or expansion of speci c memory cell populations could predispose certain patients to develop local in ammation within the neo-rectum. Therefore, the aim was to study the expression of the lymphocyte activation markers CD27, CD30, CD25 and CD69 on the CD45RO ‡ memory cell subset of isolated peripheral blood mononuclear cells (PBMC), soluble CD30 levels and mucosal CD30 expression in patients with pouchitis and in controls. Methods: Flow cytometry was performed on PBMC isolated from patients with pouchitis (n = 9), without pouchitis (n = 10) and normal controls (n = 9). Serum CD30 was measured in patients with pouchitis (n = 25), without pouchitis (n = 26) and normal controls (n = 20) by ELISA. CD30 expression was quanti ed in pouchitis (n = 15) and normal pouch (n = 15) mucosa using a three-stage immunoperoxidase method. Results: Naṏve CD45RO-CD27 ‡ PBMC were signi cantly decreased in pouchitis (25.6%) compared to normal controls (34.4%), (P = 0.03). CD30, CD25 and CD69 subsets did not differ between the groups. Serum CD30 was increased in pouchitis patients 58 (1-380) U/ ml compared to non-pouchitis 16.5 (1-290) U/ml, P = 0.007, and normal controls 11 (2-80) U/ml, P = 0.0005. In the mucosa, the numbers of CD30 ‡ cells were increased in pouchitis compared to nonin amed pouches (P = 0.02). Conclusions: Increased sCD30 in pouchitis is associated with elevated mucosal expression. Of the activation markers studied, only the circulating naṏve CD27 ‡ population differed in pouchitis patients compared with controls. The observed decrease in this cell type may re ect antigen priming and subsequent loss of CD27 implying that antigen driven activation of speci c T cell subsets may occur in pouchitis.

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Section: Discussionsupporting

confidence: 53%

Altered Expression of the Lymphocyte Activation Markers CD30 and CD27 in Patients with Pouchitis

Forbes

2001

Scandinavian Journal of Gastroenterology

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“…Although CD4+ and CD8+ T cells contain counterpart functions, such immuno-activation as helper/inducer T cells and immuno-suppression as regulatory T cells, the CD4/CD8 ratio is one of the most reflective markers for immune activation [14,26] . In the present study, the CD4/CD8 ratio in the appendix significantly increased both in A-Lt and A-Lt/Ap compared with that in controls ( Table 2).…”

Section: Discussionmentioning

confidence: 99%

Appendix is a priming site in the development of ulcerative colitis

Matsushita

Takakuwa²,

Matsubayashi³

et al. 2005

WJG

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AIM:The role of the appendix has been highlighted in the pathogenesis of ulcerative colitis (UC). The aims of this study were to elucidate the immuno-imbalances in the appendix of UC patients, and to clarify the role of the appendix in the development of UC. METHODS:Colonoscopic biopsy specimens of the appendix, transverse colon, and rectum were obtained from 86 patients with UC: active pancolitis (A-Pan; n = 15), active left-sided colitis (A-Lt; n = 25), A-Lt with appendiceal involvement (A-Lt/Ap; n = 10), inactive pancolitis (I-Pan; n = 14), and inactive left-sided colitis (I-Lt; n = 22), and from controls. In the isolated mucosal T cells, the CD4/CD8 ratio and proportion of activated CD4+ T cells were investigated, and compared with controls. RESULTS:In the appendix, the CD4/CD8 ratio significantly increased in A-Lt and A-Lt/Ap. The ratio in the appendix also tended to increase in A-Pan. In the rectum, the ratio significantly increased in all UC groups. In the appendix, the proportion of CD4+CD69+ (early activation antigen) T cells significantly increased in all UC groups. In the rectum, the proportion of CD4+CD69+ T cells significantly increased only in A-Pan. The proportion of CD4+HLA-DR+ (mature activation antigen) T cells significantly increased only in the rectum of A-Pan, but not in the other areas of any groups. CONCLUSION:The increased CD4/CD8 ratio and predominant infiltration of CD4+CD69+ T cells in the appendix suggest that the appendix is a priming site in the development of UC.

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“…The difference in these immune‐cell populations and the pathological changes of DSS‐induced acute colonic inflammation was well correlated with the development of both RALN and DSS‐induced lymph nodules. It is believed that some antigen stimulates epithelial cells and macrophages located in subepithelial regions and, thereafter, T cells and macrophages were activated in case of inflammatory bowel diseases 1,16–19 . The accumulation of activated macrophages and T cells producing a number of cytokines was associated with the degree of inflammation.…”

Section: Discussionmentioning

confidence: 99%

“…It is believed that some antigen stimulates epithelial cells and macrophages located in subepithelial regions and, thereafter, T cells and macrophages were activated in case of inflammatory bowel diseases. 1,[16][17][18][19] The accumulation of activated macrophages and T cells producing a number of cytokines was associated with the degree of inflammation. Collectively, the immunological reaction of immune cells consisting of CD4 + lymphocytes and macrophages in DSS-induced lymph nodules is significantly associated with the expansion of colitis from the lower region of the rectum to the upper region of the rectum and colon.…”

Section: Discussionmentioning

confidence: 99%

Correlation of rectum‐associated lymph nodules with the development of experimentally induced acute colonic inflammation in rats

Ichikawa

Yamashita

2001

J of Gastro and Hepatol

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Activated CD4+ and CDS+ Cells in the Colonic Mucosa of Ulcerative Colitis Patients: Their Relationship to HLA-DR Antigen Expression on the Colonic Epithelium and Serum Soluble CD25 Levels

Cited by 10 publications

References 14 publications

Altered Expression of the Lymphocyte Activation Markers CD30 and CD27 in Patients with Pouchitis

Altered Expression of the Lymphocyte Activation Markers CD30 and CD27 in Patients with Pouchitis

Appendix is a priming site in the development of ulcerative colitis

Correlation of rectum‐associated lymph nodules with the development of experimentally induced acute colonic inflammation in rats

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