Actions of Proteolytic Enzymes on Agglutinability of M and N Blood-group Red Cells

Sagisaka, Kaoru; Tokiwa, Kazuo; Yoshioka, Naofumi

doi:10.1620/tjem.106.191

Tohoku J. Exp. Med.

1972

DOI: 10.1620/tjem.106.191

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Actions of Proteolytic Enzymes on Agglutinability of M and N Blood-group Red Cells

Kaoru Sagisaka

Kazuo Tokiwa

Naofumi Yoshioka

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Cited by 5 publications

(1 citation statement)

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“…On the other hand, it has been pointed out that the enzyme treatment evidently changes M and N agglutinogens so that both M and N red cells are similarly detected as MN group (Wiener andKatz 1951, Rosenfield andVogel 1951). In the previous paper (Sagisaka et al 1972), the following facts were revealed: The trypsin-treated red cells were grouped as MN when usual rabbit antisera were used, but the eluted anti-M antibody, which was confirmed to be IgG immunoglobulin, could not agglutinate the trypsin-treated M,N and MN red cells. Therefore, it appeared that changes in agglutinability of the trypsin-treated red cells were caused by each of the class antibodies.…”

mentioning

confidence: 94%

Further Studies on Agglutinability of M and MN Blood Group Red Cells Treated with a Proteolytic Enzyme

Sagisaka

Tokiwa

Yoshioka

1972

Tohoku J. Exp. Med.

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mentioning

confidence: 94%

Further Studies on Agglutinability of M and MN Blood Group Red Cells Treated with a Proteolytic Enzyme

Sagisaka

Tokiwa

Yoshioka

1972

Tohoku J. Exp. Med.

Self Cite

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Formaldehyde in the Pathogenesis of Hemodialysis‐related Anti‐N Antibodies

White¹,

Miller²,

Kaehny³

1977

Transfusion

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The role of formaldehyde (CH2O) in the pathogenesis of anti-N-like antibodies found in patients on chronic hemodialysis was investigated. In vitro studies were performed by incubating MM cells in varying concentrations of CH2O for variable time periods, corresponding to conditions that occur in dialysis. This MM cells acquired reactivity to anti-N antibody. The antigenic shift is felt to occur from the interaction of CH2O with the terminal N-acetylneuraminic acid (sialic acid) groups of M substance, resulting in the exposure to an altered-N antigen that is immunogenically active. Through a similar mechanism, CH2O may also directly modify N substance.

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Frequencies and Specificities of “Enzyme-Only” Detected Erythrocyte Alloantibodies in Patients Hospitalized in Austria: Is an Enzyme Test Required for Routine Red Blood Cell Antibody Screening?

Enko

Habres

Wallner

et al. 2014

Journal of Blood Transfusion

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The aim of this study was to determine the frequencies and specificities of “enzyme-only” detected red blood cell (RBC) alloantibodies in the routine antibody screening and antibody identification in patients hospitalized in Austria. Routine blood samples of 2420 patients were investigated. The antibody screening was performed with a 3-cell panel in the low-ionic strength saline- (LISS-) indirect antiglobulin test (IAT) and with an enzyme-pretreated (papain) 3-cell panel fully automated on the ORTHO AutoVue Innova System. The antibody identification was carried out manually with an 11-cell panel in the LISS-IAT and with an enzyme-pretreated (papain) 11-cell panel. In total 4.05% (n = 98) of all patients (n = 2420) had a positive RBC antibody screening result. Of them 25.51% (25/98) showed “enzyme-only” detected specific or nonspecific RBC alloantibodies. Rhesus and Lewis system antibodies were found the only specificities of “enzyme-only” RBC alloantibodies: all in all 4.8% (4/98) were detected with anti-E, 3.06% (3/98) with anti-Lea, 3.06% (3/98) with anti-D after anti-D prophylaxis and 1.02% (1/98) with anti-e. In total, 14.29% (14/98) showed a nonspecific RBC alloantibody result with the enzyme test. The results of the present study demonstrate that a high number of unwanted positive reactions with the enzyme technique overshadows the detection of “enzyme-only” RBC alloantibodies. (Trial Registration: K-37-13).

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Actions of Proteolytic Enzymes on Agglutinability of M and N Blood-group Red Cells

Cited by 5 publications

References 5 publications

Further Studies on Agglutinability of M and MN Blood Group Red Cells Treated with a Proteolytic Enzyme

Further Studies on Agglutinability of M and MN Blood Group Red Cells Treated with a Proteolytic Enzyme

Formaldehyde in the Pathogenesis of Hemodialysis‐related Anti‐N Antibodies

Frequencies and Specificities of “Enzyme-Only” Detected Erythrocyte Alloantibodies in Patients Hospitalized in Austria: Is an Enzyme Test Required for Routine Red Blood Cell Antibody Screening?

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