Further Studies on Agglutinability of M and MN Blood Group Red Cells Treated with a Proteolytic Enzyme

Sagisaka, Kaoru; Tokiwa, Kazuo; Yoshioka, Naofumi

doi:10.1620/tjem.106.373

Cited by 2 publications

(1 citation statement)

References 8 publications

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“…Human IgM anti-A serum was purchased from Ortho Diagnostic (Raritan, NJ, USA). Rabbit IgG anti-M was prepared as described previously (Sagisaka et al 1972). …”

Section: Methodsmentioning

confidence: 99%

Heat and chemical stability of blood group-specific immunoglobulins in the state of immunecomplex.

Sagisaka

Noguchi

Funayama

et al. 1986

Tohoku J. Exp. Med.

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“…Human IgM anti-A serum was purchased from Ortho Diagnostic (Raritan, NJ, USA). Rabbit IgG anti-M was prepared as described previously (Sagisaka et al 1972). …”

Section: Methodsmentioning

confidence: 99%

Heat and chemical stability of blood group-specific immunoglobulins in the state of immunecomplex.

Sagisaka

Noguchi

Funayama

et al. 1986

Tohoku J. Exp. Med.

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Preparation of Anti-M and -N IgG Antibodies Using Proteolytic Enzyme-treated M Group Red Cells

Sagisaka

Tokiwa

Katsura

1972

Tohoku J. Exp. Med.

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It was reported previously that proteolytic enzyme-treated M red cells lost their reactivity completely with only anti-M IgG antibody.In this paper, M red cells treated with trypsin for 45 min or 3 hr were used to prepare group-specific anti-M IgG and -N IgG antibodies as an adsorbent.It was revealed that M red cells treated for 3 hr could adsorb anti-N antibody in anti-M IgG fraction obtained from crude anti-M serum by DEAEcellulose column chromatography.The potency of the red cells to adsorb anti-N IgG antibody was similar to that of normal N red cells. Anti-N IgG antibody was prepared by heat-eluting trypsin-treated M red cells sensitized with anti-N fraction.However, the experiments with M red cells treated for 45 min showed unsatisfactory results.It was due to the red cells which could still react with high-titered anti-M IgG antibody but not sufficiently with anti-N IgG antibody.-MN blood groups; IgG; proteolytic enzymeThe MN blood grouping has been usually performed with rabbit anti-M and -N sera in which non-specific agglutinins were adsorbed by the red cells lacking the respective agglutinogens. Although anti-M and -N agglutinins from human, animals other than rabbit and plant seed are practically used, it has been pointed out that these agglutinins still contain some non-specific agglutinins (Race and Sanger 1968). In the previous paper (Sagisaka et al. 1972a), it was revealed that proteolytic enzyme-treated M red cells lost their reactivity completely with anti-M IgG antibody but became agglutinable with each of anti-M IgA and IgM antibodies and anti-N IgG, IgA and IgM antibodies. These findings suggested that trypsintreated M red cells could adsorb non-specific agglutinins in anti-M IgG and anti-N IgG fractions prepared from crude anti-M and -N sera by DEAE-cellulose column chromatography.In this paper, attempts to specify anti-M IgG fraction by adsorption and anti-N IgG fraction by adsorption-elution method using M red cells treated sufficiently with trypsin were described. MATERIALS AND METHODSAnti-M and -N sera: Rabbits weighing about 2.5 kg were daily immunized with 1 ml of 10% of O.M or ON red cells for 45 days.Immunoglobulin IgG fraction of crude

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Further Studies on Agglutinability of M and MN Blood Group Red Cells Treated with a Proteolytic Enzyme

Cited by 2 publications

References 8 publications

Heat and chemical stability of blood group-specific immunoglobulins in the state of immunecomplex.

Heat and chemical stability of blood group-specific immunoglobulins in the state of immunecomplex.

Preparation of Anti-M and -N IgG Antibodies Using Proteolytic Enzyme-treated M Group Red Cells

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