Actions of Cannabis sativa L. fixed oil and nano-emulsion on venom-induced inflammation of Bothrops moojeni snake in rats

Carvalho, Helison de Oliveira; Gonçalves, Danna Emanuelle Santos; Picanço, Karyny Roberta Tavares; Santos, Abrahão Victor Tavares de Lima Teixeira dos; Lucia, Maria Della; Hu, Xuebo; Fernandes, Caio P.; Ferreira, Irlon M.; Carvalho, José Carlos Tavares

doi:10.1007/s10787-020-00754-y

Cited by 8 publications

(9 citation statements)

References 54 publications

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“…By associating the glycemic and insulin levels of the groups, it is possible to infer that the treatments with CSO and NCS contributed substantially to the improvement of the diabetic condition by increasing the elevation of insulin levels. The study carried out by de Oliveira Carvalho et al (2021) demonstrated that the treatment with formulations containing extract of the species Calophyllum brasiliense presented an anti-diabetic effect by restoring the β-pancreatic cells and contributing to the decrease of glycemic levels. Dewanjee et al (2020) report that plant-derived antidiabetic agents can reduce diabetic complications through glycemic control, antioxidant mechanisms, and inhibition of mechanisms that lead to the destruction of pancreatic β-cells mediated by pathological processes involved in inflammation, fibrosis, and apoptosis.…”

Section: Discussionmentioning

confidence: 99%

“…injection of streptozotocin (STZ) (SIGMA-Aldrich Inc., St. Louis, MO, USA) dissolved in 0.01 M sodium citrate buffer (pH 4.5), with an administered dose of 55 mg/kg in a volume of 1 mL/kg of body weight. Four days after STZ injection, animals with blood glucose above 300 mg/dL were considered diabetic (de Oliveira Carvalho et al, 2021).…”

Section: Induction and Experimental Design Of Dmmentioning

confidence: 99%

“…Diabetes mellitus (DM) is a syndrome that interferes with energy metabolism, caused by factors such as deficiency in insulin production and reduced tissue sensitivity to this hormone. Such factors lead to chronic hyperglycemia, characterized by high glucose levels in the bloodstream, which directly interferes with lipid metabolism, causing dyslipidemia, a condition in which changes in plasma lipid levels, such as cholesterol, triglycerides, and lipoproteins, are evidenced (de Oliveira Carvalho et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

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Cannabis sativa L. Fixed Oil and its Nanoemulsion: Effect on Diabetes and Dyslipidemia Induced in Rats

de Oliveira Carvalho,

de Melo Santos,

de Lima Teixeira dos Santos

et al. 2024

Pharmacognosy Magazine

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Background: Diabetes Mellitus (DM) is a syndrome that interferes with energy metabolism and is caused by a decrease and dysfunction of insulin, leading to chronic hyperglycemia. DM associated with dyslipidemia is a severe health risk, increasing the chance of cardiovascular events, such as acute myocardial infarction and stroke. The Cannabis sativa L. fixed oil (CSO) is composed of unsaturated fatty acids and can be crucial in treating metabolic alterations. In addition, the nanoemulsion of C. sativa oil (NCS) has advantages in optimizing treatments. Objectives: This study aimed to evaluate the effects of treatments with CSO and its nanoemulsion (NCS) on induced diabetes and dyslipidemia in Wistar rats. Materials and methods: CSO’s physical-chemical and chromatographic characterization was performed, followed by the preparation of an NE containing 6% CSO. DM was induced in Wistar rats by intraperitoneal injection of streptozotocin (STZ) at a 55 mg/kg dose. Four days later, animals with blood glucose levels exceeding 300 mg/dL were considered diabetic. The rats were then divided into five groups ( n = 5) and treated orally. The groups included a normoglycemic control group (NOR), a diabetic control group, a group treated with metformin (100 mg/kg), a group treated with CSO (400 mg/kg), and a group treated with nanoemulsion (NCS 200 mg/kg). Subsequently, the pancreas’s clinical, biochemical, and histopathological parameters were evaluated. Results: In the chemical profile of CSO, it was observed the majority composition of palmitoleic (14.58%), oleic (12.50%), linoleic (42.40%), and linolenic (8.55%) acids. The results demonstrated that the induction of DM by STZ could reproduce the typical symptoms and clinical signs of DM. It was observed that treatments with CSO and NCS showed a significant improvement ( p < .001) in polydipsia, polyuria, and loss of body mass, as well as a significant reduction ( p < .001) of glucose levels in urine and blood and serum lipids. Histopathology of the pancreas revealed that treatment with CSO and NCS showed an increase in the number of cells in the islets of Langerhans and a decrease in regions devoid of cells, indicating possible cell regeneration. Moreover, insulin levels were significantly increased ( p < .05) in the preferred groups. With dyslipidemia induced by Triton (Tyloxapol), it was observed that the treatment with CSO and NCS significantly decreased the levels of triglycerides ( p < .05) and cholesterol ( p < .001), as well as low-density lipoproteins (LDL) ( p < .01). Conclusion: Treatment with CSO and NCS under the conditions of this study demonstrated an anti-diabetic effect and the ability to act in the reduction of triglyceride, cholesterol, and LDL levels. In this respect, treatments with CSO and NCS act in the control of DM, as well as in the prevention of cardiovascular diseases.

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Section: Discussionmentioning

confidence: 99%

Section: Induction and Experimental Design Of Dmmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cannabis sativa L. Fixed Oil and its Nanoemulsion: Effect on Diabetes and Dyslipidemia Induced in Rats

de Oliveira Carvalho,

de Melo Santos,

de Lima Teixeira dos Santos

et al. 2024

Pharmacognosy Magazine

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“…28 Low-dose nano-drugs can reduce the aggregation of inflammatory cells to improve inflammation and reduce cytotoxicity. 29 Nano-sized peroxidase has higher stability and anti-inflammatory 30 Literature showed that Nano-sized curcumin could improve its biological safety and inhibit inflammation and apoptosis of pancreatic islet B cells. 31 In this study, Nano PTL was synthesized using a Kolliphor HS-15, MCT with PEG and PTL.…”

Section: Ptl Canmentioning

confidence: 99%

Nano Parthenolide Improves Intestinal Barrier Function of Sepsis by Inhibiting Apoptosis and ROS via 5-HTR2A

Guo¹,

She²,

Tan³

et al. 2023

IJN

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Background Intestinal barrier dysfunction is an important complication of sepsis, while the treatment is limited. Recently, parthenolide (PTL) has attracted much attention as a strategy of sepsis, but whether nano parthenolide (Nano PTL) is therapeutic in sepsis-induced intestinal barrier dysfunction is obscured. Methods In this study, cecal ligation and puncture (CLP)-induced sepsis rats and lipopolysaccharide (LPS)-stimulated intestinal epithelial cells (IECs) were used to investigate the effect of PTL on intestinal barrier dysfunction. Meanwhile, we synthesized Nano PTL and compared the protective effect of Nano PTL with ordinary PTL on intestinal barrier function in septic rats and IECs. Network pharmacology and serotonin 2A (5-HTR2A) inhibitor were used to explore the mechanism of PTL on the intestinal barrier function of sepsis. Results The encapsulation rate of Nano PTL was 95±1.5%, the drug loading rate was 11±0.5%, and the average uptake rate of intestinal epithelial cells was 94%. Ordinary PTL and Nano PTL improved the survival rate and survival time of septic rats, reduced the mean arterial pressure and the serum level of inflammatory cytokines, and protected the liver and kidney functions in vivo, and increased the value of transmembrane resistance (TEER) reduced the reactive oxygen species (ROS) and apoptosis in IECs in vitro through 5-HTR2A. Nano PTL had better effect than ordinary PTL. Conclusion Ordinary PTL and Nano PTL can protect the intestinal barrier function of septic rats by inhibiting apoptosis and ROS through up-regulating 5-HTR2A, Nano PTL is better than ordinary PTL.

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“…According to related literature, the stability of nanoemulsions is related to the droplet size of the emulsion which means smaller droplet can avoid aggregation and flocculation, and thus having better stability. 24…”

Section: Storage Stabilitymentioning

confidence: 99%

Nanoemulsion for Improving the Oral Bioavailability of Hesperetin: Formulation Optimization and Absorption Mechanism

Zeng

Wang

Tian

et al. 2021

Journal of Pharmaceutical Sciences

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This study aimed to prepare a nanoemulsion vehicle to improve the oral bioavailability of hesperetin. The pseudo-ternary phase diagrams and the RSM were used to optimize nanoemulsion parameters. Compared with hesperetin suspension, the AUC 0-t and C max of nanoemulsion were increased by 5.67-fold and 2.64-fold, respectively. The proportion of lymphatic transport reached 87.72%, the cumulative absorption amount of jejunum, ileum, and duodenum increased by 1.1-fold, 1.92-fold, and 1.5-fold, respectively. The results implied that hesperetin nanoemulsion could effectively improve the bioavailability of hesperetin by increasing lymphatic transport and enhancing intestinal permeability. Therefore, nanoemulsion is a potential method to improve the bioavailability of hesperetin, which has strong practicability.

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Actions of Cannabis sativa L. fixed oil and nano-emulsion on venom-induced inflammation of Bothrops moojeni snake in rats

Cited by 8 publications

References 54 publications

Cannabis sativa L. Fixed Oil and its Nanoemulsion: Effect on Diabetes and Dyslipidemia Induced in Rats

Cannabis sativa L. Fixed Oil and its Nanoemulsion: Effect on Diabetes and Dyslipidemia Induced in Rats

Nano Parthenolide Improves Intestinal Barrier Function of Sepsis by Inhibiting Apoptosis and ROS via 5-HTR2A

Nanoemulsion for Improving the Oral Bioavailability of Hesperetin: Formulation Optimization and Absorption Mechanism

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