Action of Testosterone on the Differentiation and Secretory Activity of a Target Organ: The Submaxillary Gland of the Mouse

Chrétien, M

doi:10.1016/s0074-7696(08)60101-1

Cited by 161 publications

(82 citation statements)

References 137 publications

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“…The submandibular glands of mice exhibit two distinct secretory compartments, represented by the acini and by the convoluted granular tubules. The acini, which are the terminal portions of the adenomere, consist of seromucous cells, where as the convoluted tubules, which are structure between the intercalated ducts and the striated ducts, consist of serous cells (Jacob, 1959;Raynaud, 1960;Caramia, 1966;Gresik & MacRae, 1975;Chretien, 1977;Pinkstaff, 1980). The convoluted granular tubules are considerably more developed in males than in females, characterizing the marked sexual dimorphism existing in the submandibular glands of these rodents (Lacassagne, 1940;Raynaud, 1960;Chretien, 1977;Pardini, 1985;Jayasinghe, Cope & Jacob, 1990;Sawada & Noumura, 1991;Assis et al , 1994).…”

Section: Discussionmentioning

confidence: 99%

“…We obtained diameters of 49.5 and 36.1 'um for males and females, respectively. The values reported in the literature range from 47.0 to 60.01am and from 31.4 to 40.0ium for males and females, respectively (Lacassagne, 1940;Lacassagne & Chamorro, 1940;Harvey, 1952;Raynaud, 1960;Travill, 1966;Disher, 1967;Chretien, 1977). Gresik & Mac Rae (1975), working with material fixed in glutaraldehyde-osmium and embedded in Epon resin, obtained 120.0 and 80.0 [tm for males and females, respectively.…”

Section: Compartmental Volumetric Dimensionsmentioning

confidence: 99%

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Stereological Study of the Sexual Dimorphism in Mouse Submandibular Glands

Pardini

Taga

1996

Okajimas Folia Anatomica Japonica

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Section: Discussionmentioning

confidence: 99%

Section: Compartmental Volumetric Dimensionsmentioning

confidence: 99%

Stereological Study of the Sexual Dimorphism in Mouse Submandibular Glands

Pardini

Taga

1996

Okajimas Folia Anatomica Japonica

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“…The epithelial cells of GCT have abundant secretory granules that contain a variety of biologically active peptides, including nerve growth factor (NGF), epidermal growth factor (EGF), transforming growth factor β , renin, and kallikrein (Barka 1980;Gresik 1994). Castration of the animals readily causes involution of the GCT accompanied by conversion of the phenotype of GCT cells into that of striated duct (SD) cells, whereas administration of androgens to females or castrated males causes the opposite phenomenon (Caramia 1966;Chretien 1977). Such androgen-induced GCT differentiation is accompanied by an upregulation of GCT-specifi c gene expression (Gubits et al 1986).…”

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confidence: 99%

Coexpression of Menin and JunD during the Duct Cell Differentiation in Mouse Submandibular Gland

Hipkaeo

Sakulsak

Wakayama

et al. 2008

Tohoku J. Exp. Med.

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In the submandibular gland (SMG) of mice, a duct portion called the granular convoluted tubule (GCT) is developed preferentially in males with puberty. This sexual dimorphism is androgen-dependent, but the underlying molecular mechanisms are unclear. We have demonstrated that the expression of a transcription factor JunD is regulated in association with the androgen-induced differentiation of GCT cells from striated duct (SD) cells. Menin, a nuclear protein encoded by the MEN1 tumor-suppressor gene, is known to bind JunD, thereby inhibiting its activity. In the present study, we examined the expression of menin in the mouse SMG by use of Northern blotting, Western blotting, and immunohistochemistry. Immunoreactivity for menin was higher in the female than male gland, and localized to the nuclei of intercalated duct cells and a subpopulation of SD cells. In contrast, GCT cells in males appeared negative for menin. The levels of menin in the SMG were increased with castration in males and decreased by repeated administration of testosterone to females or to castrated males. After a single administration of testosterone to females, many SD cells newly gained nuclear menin, which was lost as the cells converted to GCT cells by 48 hrs. These patterns of the expression and localization of menin were quite similar to those of JunD. Furthermore, the coimmunoprecipitation analysis of the SMG homogenates indicated that menin binds JunD in vivo. The present study suggests that the JunD-menin complex plays signifi cant roles in the androgen-dependent differentiation of the duct system in the mouse SMG.

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“…The sexual dimorphism of the granular convoluted tubule (GCT) cells of the mouse submandibular gland (SMG) has been repeatedly documented, and a large body of experimental work has established that GCT cells are under the multihormonal control of androgens, thyroid hormones, and adrenocortical hormones (reviewed by Raynaud, 1960;Chrétien, 1977;Young and van Lennep, 1978;Pinksterff, 1980;Gresik, 1994;. Generally, these hormones act as strong promotors of the GCT phenotype, and strong inducers of GCT-specific secretory products, such as EGF (epidermal growth factor), NGF (nerve growth factor), renin, and products of kallikrein gene family members.…”

mentioning

confidence: 99%

Role of thyroid hormone in the initiation of EGF (epidermal growth factor) expression in the sublingual gland of the postnatal mouse

et al. 2005

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The effect of triiodo-L-thyronine (T 3 ) and propylthiouracil (PTU) on the initiation of epidermal growth factor (EGF) expression in the sublingual glands (SLGs) of postnatal mice was investigated by indirect enzyme-labeled and immunogold antibody methods for light and electron microscopy, respectively. In normal males, EGF immunoreactivity first appeared in a few scattered granular cells of striated ducts (SDs) at 5 weeks of age, and the immunoreactive cells had increased in number at 6 weeks of age. No EGF expression was observed in the glands of females at any ages examined. When T 3 (1 mg/kg body weight) was given to males every other day for 2 weeks before examination, EGF expression began earlier; the immunoreactive granular cells were first detected at 4 weeks of age, and at later ages they were markedly increased in number compared to those of normal males. Moreover, T 3 was capable of inducing EGF in the female glands. After T 3 was administered to females in the same manner as in males, a few immunoreactive cells were first detected at 5 weeks of age, and increased numbers were detected at later ages. By contrast, when PTU (1 mg/kg body weight) was given to male mice every other day for 2 weeks before examination, the EGF-immunoreactive cells were markedly decreased in number compared to those of normal males of the same age. Electron microscopy revealed that many SD cells contained secretory granules, and that these cells constituted the granular striated tubule (GST) in a portion of SDs, but they were undetectable by light microscopy, because their secretory granules were minimal in size and few in number. Gold-labeling of EGF was confined to the secretory granules of scattered granular cells, whose secretory granules were far larger in size and more abundant than those of the GST cells. These results suggest that thyroid hormone is essential to differentiation of the cellular phenotype of GST precursor cells into typical granular cells (detectable by light microscopy) that express EGF in the mouse SLG, showing a close resemblance to the submandibular granular convoluted tubule cells.

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Action of Testosterone on the Differentiation and Secretory Activity of a Target Organ: The Submaxillary Gland of the Mouse

Cited by 161 publications

References 137 publications

Stereological Study of the Sexual Dimorphism in Mouse Submandibular Glands

Stereological Study of the Sexual Dimorphism in Mouse Submandibular Glands

Coexpression of Menin and JunD during the Duct Cell Differentiation in Mouse Submandibular Gland

Role of thyroid hormone in the initiation of EGF (epidermal growth factor) expression in the sublingual gland of the postnatal mouse

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