Action of sisal (Agave sisalana, Perrine) extract in the in vitro development of sheep and goat gastrointestinal nematodes

Silveira, Roberta Xavier da; Chagas, A. C. de S.; Botura, Mariana Borges; Batatinha, Maria José Moreira; Katiki, Luciana Morita; Carvalho, Carlos Alberto de; Beviláqua, Cláudia Maria Leal; Branco, Alexsandro; Machado, E.A.A.; Borges, Simone L.; Almeida, Maria Ângela Ornelas de

doi:10.1016/j.exppara.2012.03.018

Cited by 21 publications

(12 citation statements)

References 33 publications

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“…Some studies led by our research group describe the use of sisal waste to obtain extracts with biological activities such as larvicidal [20], antimicrobial [21], ovicidal [22] and antiparasitic [23]. In this study, we show the application of the chemical procedures to isolate chemicals (Figure 1) from A. sisalana waste with a future possibility to generate added value to the sisal agroindustry.…”

Section: Introductionmentioning

confidence: 83%

Chemicals from Agave sisalana Biomass: Isolation and Identification

Santos

Vieira

Braz-Filho

et al. 2015

IJMS

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Agave sisalana (sisal) is known worldwide as a source of hard fibers, and Brazil is the largest producer of sisal. Nonetheless, the process of removing the fibers of the sisal leaf generates 95% waste. In this study, we applied chemical sequential steps (hydrothermal extraction, precipitation, liquid-liquid extraction, crystallization, SiO2 and Sephadex LH 20 column chromatography) to obtain pectin, mannitol, succinic acid, kaempferol and a mixture of saponins as raw chemicals from sisal biomass. The structural identification of these compounds was performed though spectrometric methods, such as Infrared (IR), Ultraviolet (UV), Mass spectrometry (MS) and Nuclear magnetic resonance (NMR). All the sisal chemicals found in this work are used by both the chemical and pharmaceutical industries as excipients or active principles in products.

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Section: Introductionmentioning

confidence: 83%

Chemicals from Agave sisalana Biomass: Isolation and Identification

Santos

Vieira

Braz-Filho

et al. 2015

IJMS

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“…The substances contained in A. indica affect parasite feeding, growth, longevity, and fertility -interfering with copulation behavior, oviposition, and egg viability (El-Shafie and Basedow, 2003;Hasan and Ansari, 2011). The antihelminthic effect of Agave sisalana was studied in goats (Domingues et al, 2010;Botura et al, 2011;Silveira et al, 2012) and sheep (Silveira et al, 2012) but these studies showed discordant results. Agave sisalana contains saponins as secondary metabolites (Francis et al, 2002).…”

Section: Methodsmentioning

confidence: 99%

Efficacy, toxicity, and lethality of plants with potential anthelmintic activity in small ruminants in Brazil

Mottin

Cruz

Neto

et al. 2019

Rev. bras. saúde prod. anim.

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SUMMARY The use of medicinal plants as a therapeutic method in the control of diseases has been increasing in animal production. In the case of small ruminants, the endoparasitic disease is a major concern, since they are considered the greater sanitary problem, especially when considering the phenomenon of parasite resistance to the medicines used. Therefore, the development of alternative methods of endoparasitary control in goats and sheep has become a pressing need. The use of plants to control endoparasites can reduce the use of chemical inputs, making more environmentally sustainable livestock, minimizing the occurrence of parasitic resistance to conventional medicines and contributing to the reduction of production costs. In this sense, several studies have been carried out in order to evaluate the in vitro and in vivo activity of plants with anthelmintic potential. However, despite the potentiality found in many studies, the results are sometimes contradictory or do not replicate in vivo the same efficacy obtained in the in vitro assays. Another relevant aspect is the low utilization of the lethality and toxicity tests, which are indispensable so that the knowledge can be spread and applied by the producers in their herds. Thus, this review aims to provide the results from studies carried out in Brazil with extract of plants with potential for control of parasitic disease in goats and sheep, describing the main evaluated plants species, mechanisms of action, preparation forms and tests of efficacy, toxicity, and lethality.

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“…Our research group has investigated the biological activity of different extracts and fractions obtained from sisal waste against nematodes of goats. In the context, the flavonoid and saponin fractions from ethyl acetate extract showed ovicidal and larvicidal effects, respectively (SILVEIRA et al, 2012;BOTURA et. al., 2013;SANTOS et al, 2015).…”

Section: Parasiticmentioning

confidence: 99%

Ovicidal activity of succinic acid isolated from sisal waste (Agave sisalana) against gastrointestinal nematodes of goats

Santos

et al. 2017

Cienc. Rural

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This study was conducted to evaluate the in vitro anthelmintic activity of the succinic acid (SA) isolated from sisal waste against gastrointestinal nematodes of goats, using the egg hatching and larvae motility assays. In addition, potential cytotoxicity of SA on Vero cell cultures was investigated by means of MTT (3-4,5-dimethylthiazol-2-yl, 2,5diphenyltetrazolium bromide) test. The SA induced a significant inhibition of egg hatching (P<0.05) at all concentrations tested (60 to 250µg mL-1), and the concentrations to inhibit 50% (EC50) and 90% (EC90) values (mean ± standard deviation) were 90.3±2.8 and 130.6±3.5µg mL-1, respectively. The SA has not shown larvicidal activity. The SA was less toxic to the Vero cells, with the mean percentage of cell viability equal to 85±6.2% at the concentration of 130µg mL-1. The results suggested that SA has potential anthelmintic effect; although, more research is needed to confirm its activity in vivo.

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Action of sisal (Agave sisalana, Perrine) extract in the in vitro development of sheep and goat gastrointestinal nematodes

Cited by 21 publications

References 33 publications

Chemicals from Agave sisalana Biomass: Isolation and Identification

Chemicals from Agave sisalana Biomass: Isolation and Identification

Efficacy, toxicity, and lethality of plants with potential anthelmintic activity in small ruminants in Brazil

Ovicidal activity of succinic acid isolated from sisal waste (Agave sisalana) against gastrointestinal nematodes of goats

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