Action of silver nanoparticles towards biological systems: cytotoxicity evaluation using hen's egg test and inhibition of Streptococcus mutans biofilm formation

Freire, Priscila L.L.; Stamford, Thayza Christina Montenegro; Albuquerque, Allan J.R.; Sampaio, F. C.; Cavalcante, Horacinna M.M.; Macêdo, Rui Oliveira; Galembeck, André; Flores, Miguel Angel Pelágio; Rosenblatt, Aronita

doi:10.1016/j.ijantimicag.2014.09.007

Cited by 45 publications

(34 citation statements)

References 13 publications

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“…The use of nanotechnology for the production of antibacterial agents aiming to control and treat bacterial infections has already been applied [34][35][36][37], corroborating our results. The silver nanocomposite developed can be an alternative to conventional antibiotics, with special importance in multidrug-resistant bacteria [38,39].…”

Section: Silver Nanocomposite Biosynthesissupporting

confidence: 86%

Silver Nanocomposite Biosynthesis: Antibacterial Activity against Multidrug-Resistant Strains of Pseudomonas aeruginosa and Acinetobacter baumannii

et al. 2016

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Abstract:Bacterial resistance is an emerging public health issue that is disseminated worldwide. Silver nanocomposite can be an alternative strategy to avoid Gram-positive and Gram-negative bacteria growth, including multidrug-resistant strains. In the present study a silver nanocomposite was synthesized, using a new green chemistry process, by the addition of silver nitrate (1.10 −3 mol·L −1 ) into a fermentative medium of Xanthomonas spp. to produce a xanthan gum polymer. Transmission electron microscopy (TEM) was used to evaluate the shape and size of the silver nanoparticles obtained. The silver ions in the nanocomposite were quantified by flame atomic absorption spectrometry (FAAS). The antibacterial activity of the nanomaterial against Escherichia coli (ATCC 22652), Enterococcus faecalis (ATCC 29282), Pseudomonas aeruginosa (ATCC 27853) and Staphylococcus aureus (ATCC 25923) was carried out using 500 mg of silver nanocomposite. Pseudomonas aeruginosa and Acinetobacter baumannii multidrug-resistant strains, isolated from hospitalized patients were also included in the study. The biosynthesized silver nanocomposite showed spherical nanoparticles with sizes smaller than 10 nm; 1 g of nanocomposite contained 49.24 µg of silver. Multidrug-resistant strains of Pseudomonas aeruginosa and Acinetobacter baumannii, and the other Gram-positive and Gram-negative bacteria tested, were sensitive to the silver nanocomposite (10-12.9 mm of inhibition zone). The biosynthesized silver nanocomposite seems to be a promising antibacterial agent for different applications, namely biomedical devices or topical wound coatings.

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Section: Silver Nanocomposite Biosynthesissupporting

confidence: 86%

Silver Nanocomposite Biosynthesis: Antibacterial Activity against Multidrug-Resistant Strains of Pseudomonas aeruginosa and Acinetobacter baumannii

et al. 2016

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“…Apesar da ação antimicrobiana ser proporcional à quantidade de íons de prata liberados 23 , o cimento de ionômero de vidro com liga de prata de partículas (RIVA Silver ® ) encapsulado em sua composição, não apresentou o efeito antimicrobiano esperado, pois a sensibilidade bacteriana é maior quando o tamanho das nanopartículas é menor 24 , assim como o cimento de IV encapsulado do presente estudo (RIVA Self Cure ® ), que também não demonstrou atividade de inibição do crescimento contra as cepas de Staphylococcus Aureus e Escherichia coli.…”

Section: ®9unclassified

“…Apesar dos resultados desfavoráveis obtidos quanto à ação antimicrobiana dos outros cimentos ionoméricos testados na presente pesquisa contra as cepas de Staphylococcus Aureus e Escherichia coli, esses materiais não devem ser descartados da prática odontológica, visto que o íon fluoreto, além de facilitar a remineralização dos tecidos dentários durante os processos cíclicos de desmineralização e remineralização, atua nos microrganismos cariogê-nicos, alterando sua fisiologia 22,24 . Em um estudo realizado com metodologia diferente da que foi utilizada no atual estudo, avaliou--se a atividade antibacteriana de três materiais restauradores e um deles foi a Resina composta com partículas ionoméricas (Beautifil II ® ) contra o Streptococcus Mutans.…”

Section: ®9unclassified

Ação antibacteriana de materiais restauradores ionoméricos contra Staphylococcus aureus e Escherichia coli

Vidal

Araújo

Rocha

et al. 2017

Rev. da Fac. de Odontologia, UPF

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Objetivo: analisar a atividade antimicrobiana de um cimento de ionômero de vidro (IV) convencional, um cimento de IV capsulado, um cimento de IV modificado por resina e uma resina composta com partículas ionoméricas contra Staphylococcus aureus e Escherichia coli. Materiais e método: estudo laboratorial microbiológico avaliou, por meio da inibição do crescimento por ágar difusão no método do poço, a ação antimicrobiana de materiais restauradores contra Staphylococcus aureus e Escherichia Coli. Utilizaram-se 6 placas de Petri com ágar Müeller Hinton cujos poços foram preenchidos com os materiais ionoméricos manipulados conforme fabricantes (cimento de IV convencional, cimento de IV encapsulado, cimento de IV modificado por resina e resina composta com partículas ionoméricas - Ketac Molar Easy®, Riva Self Cure®, Vitremer® e Beautifil II®) e com controle (cimento de óxido de zinco e eugenol OZE e solução salina em discos de papéis). Houve incubação em estufa bacteriológica por 48 horas quando se analisou a formação ou não de halos de inibição do crescimento. Resultados: constatou-se que o cimento de IV modificado por resina apresentou atividade antibacteriana contra Escherichia coli e Staphylococcus aureus, com média de halos de 13 e 13,6mm, e o cimento controle OZE apresentou halos de 16 e 16,3mm. Os demais materiais restauradores e a solução salina não apresentaram formação de halos de inibição. Conclusão: apenas o cimento de IV modificado por resina apresentou atividade antimicrobiana contra as bactérias testadas.

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“…Laboratory studies have reported that SDF prevents the formation of Streptococcus mutans, Streptococcus sobrinus, Actinomyces naeslundii, Lactobacillus acidophilus and Lactobacillus rhamnosus biofilms (5), but its use for dental purposes is generally not encouraged, because it induces black tooth discoloration. However, the use of nanoscale has eliminated this problem, either in caries lesion or in healthy dental tissue, in addition to enhancing chemical and antimicrobial properties and reducing cell toxicity (6)(7)(8)(9)(10)(11). AgNPs also have little or no cytotoxicity (6,7) and clinical application has become possible without risks.…”

Section: Introductionmentioning

confidence: 99%

“…Bactericidal effects have also been observed for cells organized in biofilms, due to nanoparticle interference in "cell to cell" aggregation and "cell-surface" adherence (6,15). It has also been reported that the silver particles may inhibit free glycosyltransferases, the enzymes responsible for promoting glucose and cell adhesion that enables the production of glycans and extracellular polysaccharides.…”

Section: Introductionmentioning

confidence: 99%

AgNPs: The New Allies Against S. Mutans Biofilm - A Pilot Clinical Trial and Microbiological Assay

et al. 2017

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The purpose of this study was to evaluate the antimicrobial properties of a new formulation containing silver nanoparticles, named Nano Silver Fluoride (NSF), to inhibit Streptococcus mutans biofilm formation on children's dental enamel. The variations in dental biofilm pH and in the Simplified-Oral-Hygiene-Index (OHI-S) also were evaluated after the treatment with NSF. This was a randomized, double-blind, crossover and prospective pilot clinical trial study in which 12 schoolchildren, aged between 7-8 years, had their dental enamel treated with two solutions: S1 -Nano Silver Fluoride and S2 -negative control (saline solution), in different experimental moments. The dental biofilm adhered to enamel treated with NSF had lower values of S. mutans viability (absorbance) and colony forming units (CFU) than the S0 (baseline) and S2. There was a statistically significant difference between the OHI-S mean values of S0 and S1. There were no differences between the biofilm pH (both before and after the use of the test substances) and among the different groups. These properties suggest that NSF has bactericidal effect against S. mutans biofilm and it may be used for clinical control and prevention of dental biofilm formation.

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Action of silver nanoparticles towards biological systems: cytotoxicity evaluation using hen's egg test and inhibition of Streptococcus mutans biofilm formation

Cited by 45 publications

References 13 publications

Silver Nanocomposite Biosynthesis: Antibacterial Activity against Multidrug-Resistant Strains of Pseudomonas aeruginosa and Acinetobacter baumannii

Silver Nanocomposite Biosynthesis: Antibacterial Activity against Multidrug-Resistant Strains of Pseudomonas aeruginosa and Acinetobacter baumannii

Ação antibacteriana de materiais restauradores ionoméricos contra Staphylococcus aureus e Escherichia coli

AgNPs: The New Allies Against S. Mutans Biofilm - A Pilot Clinical Trial and Microbiological Assay

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