“…The frequency of discharge remained unaltered, and there was a shift of the steady potential POTENTIALS FROM MAMMALIAN MUSCLE to negativity, indicating that the recording electrode probably had entered a muscle fibre and was recording what had previously been an extracellular diphasic potential from the other side of the surface membrane. The transformation of a diphasic spike into a monophasic one as the recording electrode impales the surface membrane has been demonstrated in cortical neurones (Li, 1955).…”