Actinomycetes mediated targeting of drug resistant MRSA pathogens

Bhakyashree, Krishnamoorthy; Каннабиран, Кришнан

doi:10.1016/j.jksus.2018.04.034

Cited by 11 publications

(5 citation statements)

References 9 publications

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“…More significantly, the secondary screening of the ethylacetate extracts of the actinomycetes further affirmed the production of bioactive constituents in the isolates. Similar to the reports of Bhakyashree and Kannabiran (23), the extracts of the isolates, especially, Streptomyces glauciniger and Streptomyces griseoplanus, remarkably inhibited the growth of both methicillin resistant and sensitive Staphylococcus aureus strains. MRSA is one of the high priority pathogens causing lifethreatening diseases in invalids and convalescents.…”

Section: Discussionsupporting

confidence: 86%

Potentials of Actinomycetes from Reserved Environments as Antibacterial Agents Against Drug-Resistant Clinical Bacterial Strains

Ahmed

Daniel

Gbala

et al. 2020

Ethiop J Health Sci

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BACKGROUND: Increased antibiotic resistant properties of pathogens has led to a pronounced search for new effective antibiotics from microbes in diverse ecological niches. This study focused on isolating actinomycetes from soil of reserved areas and profiling them for antibacterial potentials.METHODS: The isolates (IS-2, IS-4, IS-6, IS-10, IS-14) were assessed for antagonistic activity against ten multi-drug resistant bacterial strains (Gram positive and negative) by cross streak and well diffusion methods.RESULTS: During the primary screening, four of the isolates showed good antagonistic activity against the test strains. Notably, all the bacterial strains except Pseudomonas aeruginosa responded sensitively to at least one of the actinomycetes screened. The crude extracts of the secondary metabolites of the four actinomycetes (IS-2, IS-4, IS-6, IS-10) with considerably significant antagonistic activities inhibited the growth of all the bacterial strains efficiently. All the test bacterial strains were sensitive to at least one of the extracts at a concentration of 100μg/ml. The minimum inhibitory concentration of the extracts against the isolates ranged from 12.5 - 25μg/ml. The crude extracts of IS-4 and IS-6 identified as Streptomyces glauciniger NBRC 100913 and Streptomyces griseoplanus NRRL-ISP 5009 by I6s rRNA sequencing, showed higher antibacterial activities against the bacterial strains. Significantly, the ethyl acetate crude extract of the actinomycetes demonstrated better antibacterial activities than the standard antibiotics (ofloxacin, amoxicillin/clavulanate, cefuroxime and ceftriaxone).CONCLUSION: This study reports remarkable anti-MRSA activities as well as broad spectrum antibacterial potentials of extracts of Streptomyces spp. worthy of further exploration.

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Section: Discussionsupporting

confidence: 86%

Potentials of Actinomycetes from Reserved Environments as Antibacterial Agents Against Drug-Resistant Clinical Bacterial Strains

Ahmed

Daniel

Gbala

et al. 2020

Ethiop J Health Sci

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“…Similarly, Ameen, et al 61 reported the antibacterial activity of thirty endophytic actinobacterial strains against antibiotic-resistant bacteria. Ethyl acetate extracts of actinobacteria inhibited the growth of Gram positive and negative bacteria 62 , 63 . The methanolic extract of KUMS-B3 had a moderate inhibitory activity against S. aureus .…”

Section: Discussionmentioning

confidence: 99%

Identification and antibacterial evaluation of endophytic actinobacteria from Luffa cylindrica

Mahdi

Bahrami

Kakaei

2022

Sci Rep

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The emergence of antibiotic-resistant bacteria has limited treatment options and led to the untreatable infections, thereby necessitating the discovery of new antibiotics to battel against bacteria. Natural products from endophytic actinobacteria (EA) serve as a reservoir for discovery of new antibiotics. Therefore, the current study focused on the isolation and antibacterial properties of EA isolated from Luffa cylindrica. Six strains were identified using morphological characterization, SEM analyses and 16S rRNA gene sequencing from the roots and leaves of the plant. They were taxonomically classified as Streptomycetaceae family. This is the first report on EA form L. cylindrica. The strains produced a chain of oval, cubed or cylindrical shaped spores with spiny or smooth surfaces. Three strains; KUMS-B3, KUMS-B4 and KUMS-B6 were reported as endophytes for the first time. Fifty percent of isolates were isolated from leaves samples using YECD medium. Our results showed that the sampling time and seasons may affect the bacterial diversity. All six strains had antibacterial activity against at least one of the tested bacteria S. aureus, P. aeruginosa, and E. coli. Among the strains, KUMS-B6 isolate, closely related to S. praecox, exhibited the highest antibacterial activity against both gram-positive and negative bacteria. KUMS-B6, KUMS-B5 and KUMS-B4 isolates strongly inhibited the growth of P. aeruginosa. Interestingly, the strains, isolated from leaves exhibited stronger antagonist activities compared to those isolated from the roots. The study revealed that the isolated strains from Luffa produce a plethora of bioactive substances that are potential source of new drug candidates for the treatment of infections.

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“…Diketopiperazines and chloramphenicol extracted from Streptomyces SUK 25, derived from the Zingiber spectabile root, seem effective towards MRSA [25]. Moreover, Bhakyashree and Kannabiran [93] reported the separation of anti-MRSA compounds from Streptomyces sp. VITBKA3 strain proteins and cell membrane biosynthesis inhibitors.…”

Section: The Important Antibiotics Isolated From Actinobacteria Against Drug-resistant Pathogensmentioning

confidence: 99%

Biodiversity of Secondary Metabolites Compounds Isolated from Phylum Actinobacteria and Its Therapeutic Applications

et al. 2021

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The current review aims to summarise the biodiversity and biosynthesis of novel secondary metabolites compounds, of the phylum Actinobacteria and the diverse range of secondary metabolites produced that vary depending on its ecological environments they inhabit. Actinobacteria creates a wide range of bioactive substances that can be of great value to public health and the pharmaceutical industry. The literature analysis process for this review was conducted using the VOSviewer software tool to visualise the bibliometric networks of the most relevant databases from the Scopus database in the period between 2010 and 22 March 2021. Screening and exploring the available literature relating to the extreme environments and ecosystems that Actinobacteria inhabit aims to identify new strains of this major microorganism class, producing unique novel bioactive compounds. The knowledge gained from these studies is intended to encourage scientists in the natural product discovery field to identify and characterise novel strains containing various bioactive gene clusters with potential clinical applications. It is evident that Actinobacteria adapted to survive in extreme environments represent an important source of a wide range of bioactive compounds. Actinobacteria have a large number of secondary metabolite biosynthetic gene clusters. They can synthesise thousands of subordinate metabolites with different biological actions such as anti-bacterial, anti-parasitic, anti-fungal, anti-virus, anti-cancer and growth-promoting compounds. These are highly significant economically due to their potential applications in the food, nutrition and health industries and thus support our communities’ well-being.

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Actinomycetes mediated targeting of drug resistant MRSA pathogens

Cited by 11 publications

References 9 publications

Potentials of Actinomycetes from Reserved Environments as Antibacterial Agents Against Drug-Resistant Clinical Bacterial Strains

Potentials of Actinomycetes from Reserved Environments as Antibacterial Agents Against Drug-Resistant Clinical Bacterial Strains

Identification and antibacterial evaluation of endophytic actinobacteria from Luffa cylindrica

Biodiversity of Secondary Metabolites Compounds Isolated from Phylum Actinobacteria and Its Therapeutic Applications

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