Actinomycetes from Moroccan habitats: isolation and screening for cytotoxic activities

Abstract

In our screening for actinomycetes showing cytotoxic activities, 8 samples were collected from various Moroccan habitats, 136 isolates were tested for their capacity to produce antibacterial compounds against gram positive bacteria. Thirty-seven strains of these isolates were active against Gram-positive bacteria. Using the following steps of primary screening: antibacterial activity, confrontation between the isolates and toxicity to Artemia salina; fifteen different isolates were used for further investigation. The aqueous extracts of Streptomyces sp. T5 and Streptomyces sp. AS8 were selected for their cytotoxic activity against Hep2, BSR and P815 cell lines, and two active compounds were observed on HPLC. The two isolates exhibited high activity against human cancer cell lines and were inactive on PBMC cell lines. Furthermore, the Streptomyces sp. T5 extract showed a proliferative activity.

Anticancer property of sediment actinomycetes against MCF–7 and MDA–MB–231 cell lines

“…Moreover, quinine derivatives viz., driamycin, daunorubicin, mitomycin C, streptonigrin and lapachol, can interfere the DNA and RNA replication and mitochondrial oxidative pathways or the formation of super oxide, peroxide and hydroxide radicals as toxic products in the cell line. Several compounds of anthroquinone families (parimycin, trioxacarcins and gutingimycin) showed antitumor activities [18][19][20] . The cytotoxic effect of actinomycete extracts seems to be the induction of the cell apoptosis, partial cellular differentiation, and degradation of fusion transcripts, antiproliferation and inhibition of distinct relations with the other groups.…”

Molecular analysis and anticancer properties of two identified isolates, Fusarium solani and Emericella nidulans isolated from Wady El–Natron soil in Egypt against Caco–2 (ATCC) cell line

“…Macroconidia were formed after [4][5][6][7] days from short multi-branched conidiophores which may form sporodochia. They are 3-septate, fusiform, cylindrical, often moderately curved, with an indistinctly pedicellate foot cell and a short blunt apical cell, (28)(29)(30)(31)(32)(33)(34)(35)(36)(37)(38)(39)(40)(41)(42) 毺m 暳 4-6 毺m). Microconidia are usually abundant, cylindrical to oval, oneto two-celled and formed from long lateral phialides, (8)(9)(10)(11)(12)(13)(14)(15)(16) 毺m 暳 2-4.5 毺m).…”

“…Several compounds of anthroquinone families showed antitumor activities [30][31][32] . The cytotoxic effect of the two fungal isolates seems to be the induction of apoptosis, partial cellular differentiation, and degradation of fusion transcripts, antiproliferation and inhibition of angiogenesis [1] .…”

“…Soil samples were numbered and put in a sterilized paper sack, transferred immediately to the Microbiology Laboratory of the Faculty of Science, Al-Azhar University (Girls Branch). One gram of soil was aseptically transferred into 99 mL of presterilized water and kept for continuous shaking (100 rpm) for 1 h. About 100 毺 L of diluted sample was transferred to molten Czabec Dox agar medium consisting of 2 g/L sodium nitrate, 0.5 g/L potassium chloride, 0.5 g/L magnesium glycerophosphate, 0.01 g/L ferrous sulphate, 0.35 g/L potassium sulphate, 30.0 g/ L sucrose, and 12.0 g/L agar (pH 6.8) and made up to 1 L with sterile water and incubated at 28 曟 for 7 days.…”

“…PCR was conducted for [30][31][32][33][34][35][36][37][38][39][40] cycles in a thermal controller (Programmable Thermal Controller; M) Research Inc., Watertown, MA). The primers used for amplification of p53 gene were shown in Table 1.…”