Actinomycetes Diversity in the Rhizosphere Soils of Different Medicinal Plants in Kolly Hills-Tamilnadu, India, for Secondary Metabolite Production

Thangapandian, V.; Ponmurugan, P.; Karuppiah, Ponmurugan

doi:10.3923/ajps.2007.66.70

Cited by 29 publications

(8 citation statements)

References 7 publications

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“…Streptomyces strains are generally the most common actinobacteria in soil (Xu et al 1996). In a survey on the rhizosphere soil of Indian medicinal plants, eight actinobacterial strains were isolated from different regions in India, all of which belonged to genus Streptomyces (Thangapandian et al 2007). Up to ten different actinobacterial genera were isolated from the rhizosphere soil of 16 Thai medicinal plant species (Khamna et al 2009a,b).…”

Section: Discussionmentioning

confidence: 99%

The rhizospheres of traditional medicinal plants in Panxi, China, host a diverse selection of actinobacteria with antimicrobial properties

Zhao

Penttinen

Chen

et al. 2012

Appl Microbiol Biotechnol

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Actinobacteria are a prolific source of antibiotics. Since the rate of discovery of novel antibiotics is decreasing, actinobacteria from unique environments need to be explored. In particular, actinobacterial biocontrol strains from medicinal plants need to be studied as they can be a source of potent antibiotics. We combined culture-dependent and culture-independent methods in analyzing the actinobacterial diversity in the rhizosphere of seven traditional medicinal plant species from Panxi, China, and assessed the antimicrobial activity of the isolates. Each of the plant species hosted a unique set of actinobacterial strains. Out of the 64 morphologically distinct isolates, half were Streptomyces sp., eight were Micromonospora sp., and the rest were members of 18 actinobacterial genera. In particular, Ainsliaea henryi Diels. hosted a diverse selection of actinobacteria, although the 16S ribosomal RNA (rRNA) sequence identity ranges of the isolates and of the 16S rRNA gene clone library were not congruent. In the clone library, 40% of the sequences were related to uncultured actinobacteria, emphasizing the need to develop isolation methods to assess the full potential of the actinobacteria. All Streptomyces isolates showed antimicrobial activity. While the antimicrobial activities of the rare actinobacteria were limited, the growth of Escherichia coli, Verticillium dahliae, and Fusarium oxysporum were inhibited only by rare actinobacteria, and strains related to Saccharopolyspora shandongensis and Streptosporangium roseum showed broad antimicrobial activity.

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Section: Discussionmentioning

confidence: 99%

The rhizospheres of traditional medicinal plants in Panxi, China, host a diverse selection of actinobacteria with antimicrobial properties

Zhao

Penttinen

Chen

et al. 2012

Appl Microbiol Biotechnol

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“…Based on the antimicrobial activity of the tested strains, the isolate AW6 has been selected for further investigation Table 2 shows the antimicrobial activity of some selected actinomycetes. Actinomycetes species have been mentioned in several reports as a source of bioactive secondary metabolites such as cytotoxic compounds (Yoo et al 2002 ; Thangapandian et al 2007 ) and antimicrobial compounds (Kumari et al 2006 ; Rizk et al 2007 ) that have the potential to control a wide range of pathogens. Millions of microorganisms, including indigenous actinomycetes, live in the marine environment and play an important role in the mineralization of complex organic matter, degradation of dead plants, plankton, and animals, removal of pollutants and toxicants, and production of primary and secondary metabolites (Genilloud et al 2011 ).…”

Section: Resultsmentioning

confidence: 99%

Bioactive secondary metabolites from marine Actinomyces sp. AW6 with an evaluation of ADME-related physicochemical properties

et al. 2022

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This study was designed to evaluate the antimicrobial, antioxidant, and cytotoxic potentials of the marine actinomycetes spp. isolated from the Red Sea water, Hurghada, Egypt. Out of 80 actinomycetes isolates, one isolate AW6 was selected based on its antioxidant activity (IC50 about 5.24 µg/mL which scavenged 91% of formed DPPH free radicals) and antimicrobial potential against E. coli, S. aureus, B. subtilis, and P. aeruginosa, A. niger, and C. albicans. The strain was identified based on phenotypic and genotypic analysis, and deposited in the GenBank with accession number OK090864.1. Cultivation of the selected strain on rice, chromatographic purification, and structural elucidation led to the isolation of two compounds C1: umbelliferone, and C2: 1-methoxy-3-methyl-8-hydroxy-anthraquinone. The antimicrobial activity of the obtained compounds showed that C1 and C2 have low antibacterial activity toward S. aureus and E. coli with no pronounced activity toward P. aeruginosa, C. albicans, and A. niger. Additionally, the antioxidant activity of C1 and C2 revealed that C2 has a good antioxidant activity, with DPPH scavenging activity reaching (55.25%), followed by C1 (30.20%). Moreover, both compounds displayed anti-Gyr-B enzyme activity with IC50 value of (3.79 ± 0.21 µM) for C1, and (IC50 = 13 ± 0.71 µM) for C2. The ADME-related physicochemical properties of the obtained compound were predicted using SwissADME web tools and the ProToxii webserver was used to estimate in silico toxicity.

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“…Based on the antimicrobial activity of the tested strains, the isolate AW6 has been selected for further investigation Table 2 shows the antimicrobial activity of some selected actinomycetes. Actinomycetes species have been mentioned in several reports as a source of bioactive secondary metabolites such as cytotoxic compounds (Yoo et al 2002, Thangapandian et al 2007) and antimicrobial compounds (Kumari et al 2006, Rizk et al 2007) that have the potential to control a wide range of pathogens. Millions of microorganisms, including indigenous actinomycetes, live in the marine environment and play an important role in the mineralization of complex organic matter, degradation of dead plants, plankton, and animals, removal of pollutants and toxicants, and production of primary and secondary metabolites (Genilloud et al 2011).…”

Section: Resultsmentioning

confidence: 99%

Bioactive secondary metabolites from marine Actinomyces sp. AW6 with an evaluation of ADME-related physicochemical properties

Abdellatif

Hamed

Ghareeb

et al. 2022

Preprint

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This study aimed to evaluate the antimicrobial, antioxidant, and cytotoxic potentials of crude extract of marine actinomycetes spp. isolated from the Red Sea water at Hurghada, Egypt. Out of 80 actinomycetes isolates, the isolate Actinomyces sp. AW6 was selected based on its antioxidant (IC50 about 5.24 µg/ml which scavenged 91% of formed DPPH free radicals) and antimicrobial potential against E. coli, S. aureus, B. subtilis, and P. aeruginosa, A. niger, and C. albicans. The strain was identified based on phenotypic and genotypic analysis and deposited in the GenBank with accession number OK090864. 1. Cultivation of the selected strain on rice, chromatographic purification, and structural elucidation led to the isolation of two compounds C1: umbelliferone and C2: 1-methoxy-3-methyl-8-hydroxy-anthraquinone. The antimicrobial activity of the obtained compounds showed that C1 and C2 have low antibacterial activity toward S. aureus and E. coli with no pronounced activity toward P. aeruginosa, C. albicans, and A. niger. Additionally, the antioxidant activity of C1 and C2 revealed that C2 has a good antioxidant activity, with DPPH scavenging activity reaching (55.25%), followed by C1 (30.20%). Moreover, both compounds displayed anti-Gyr-B enzyme activity with IC50 value of (3.79 ± 0.21 µM) for C1, and (IC50 = 13 ± 0.71µM) for C2. The ADME-related physicochemical properties of the obtained compound were predicted using SwissADME web tools and the ProToxii webserver was used to estimate in silico toxicity.

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Actinomycetes Diversity in the Rhizosphere Soils of Different Medicinal Plants in Kolly Hills-Tamilnadu, India, for Secondary Metabolite Production

Cited by 29 publications

References 7 publications

The rhizospheres of traditional medicinal plants in Panxi, China, host a diverse selection of actinobacteria with antimicrobial properties

The rhizospheres of traditional medicinal plants in Panxi, China, host a diverse selection of actinobacteria with antimicrobial properties

Bioactive secondary metabolites from marine Actinomyces sp. AW6 with an evaluation of ADME-related physicochemical properties

Bioactive secondary metabolites from marine Actinomyces sp. AW6 with an evaluation of ADME-related physicochemical properties

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