Actinomycete morphology in shaken culture

Lawton, Peter; Whitaker, Allan; Odell, D.E.; Stowell, Julian

doi:10.1139/m89-147

Cited by 15 publications

(8 citation statements)

References 9 publications

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“…To effectively treat aquifers in situ, distribution of the added microbes is paramount to ensure contact between the contaminant and the added biocatalyst (Steffan et al ). Notably, most of the bacterial strains currently known to degrade 1,4‐dioxane are actinomycetes, and these organisms are known to form filaments or mycelia, and when grown with agitation, they can form dense or loose pellets, pellicles, or other aggregates (Lawton et al ). Strain CB1190, for example, is reported to form branching aerial and vegetative mycelium (Mahendra and Alvarez‐Cohen ), and strain ENV425 forms cell clumps and attaches rapidly to surfaces (Hatzinger et al ; Webster et al ).…”

Section: Resultsmentioning

confidence: 99%

“…The limited distribution of propane to the more distant monitoring well could have been due to either the rapid consumption of propane between the sparging well and 24‐MW‐5B or the changes in the aquifer permeability caused by biomass growth (Dupins et al ) or trapped gasses (Fry et al ), which could limit transport of the added propane to the vicinity of 24‐MW‐5B. Importantly, Dupin et al () demonstrated that microbes that form aggregates or biofilms, like the actinomycetes known to degrade propane and 1,4‐dioxane (Lawton et al ), can effectively reduce aquifer porosity.…”

Section: Resultsmentioning

confidence: 99%

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Bioaugmentation and Propane Biosparging for In Situ Biodegradation of 1,4-Dioxane

Lippincott¹,

Streger²,

Schaefer³

et al. 2015

Groundwater Monit R

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Propane biosparging and bioaugmentation were applied to promote in situ biodegradation of 1,4‐dioxane at Site 24, Vandenberg Air Force Base (VAFB), CA. Laboratory microcosm and enrichment culture testing demonstrated that although native propanotrophs appeared abundant in the shallow water‐bearing unit of the aquifer (8 to 23 ft below ground surface [bgs]), they were difficult to be enriched from a deeper water‐bearing unit (82 to 90 feet bgs). Bioaugmentation with the propanotroph Rhodococcus ruber ENV425, however, supported 1,4‐dioxane biodegradation in microcosms constructed with samples from the deep aquifer. For field testing, a propane‐biosparging system consisting of a single sparging well and four performance monitoring wells was constructed in the deep aquifer. 1,4‐dioxane biodegradation began immediately after bioaugmentation with R. ruber ENV425 (36 L; 4 × 109 cells/mL), and apparent first‐order decay rates for 1,4‐dioxane ranged from 0.021 day−1 to 0.036 day−1. First‐order propane consumption rates increased from 0.01 to 0.05 min−1 during treatment. 1,4‐dioxane concentrations in the sparging well and two of the performance monitoring wells were reduced from as high as 1090 µg/L to <2 µg/L, while 1,4‐dioxane concentration was reduced from 135 µg/L to 7.3 µg/L in a more distal third monitoring well. No 1,4‐dioxane degradation was observed in the intermediate aquifer control well even though propane and oxygen were present. The demonstration showed that propane biosparging and bioaugmentation can be used for in situ treatment of 1,4‐dioxane to regulatory levels.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Bioaugmentation and Propane Biosparging for In Situ Biodegradation of 1,4-Dioxane

Lippincott¹,

Streger²,

Schaefer³

et al. 2015

Groundwater Monit R

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“…A high concentration tends to produce a dispersed form of growth while a low concentration normally results in pellet formation (Lawton et al . ).…”

Section: Discussionmentioning

confidence: 97%

Exclusion ofVibriospp. by an antagonistic marine actinomyceteStreptomyces rubrolavendulaeM56

2015

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A marine actinomycete Streptomyces rubrolavendulae M56 isolated from the sediments of Bay of Bengal and displaying biogranulation property was used for the study. The strain showed antagonistic property against vibrios, the opportunistic pathogens in aquaculture. The efficacy of the biogranules of actinomycete M56 in competitive exclusion of Vibrio spp. was tested both in vitro and in vivo. Streptomyces rubrolavendulae M56 biogranules could significantly exclude the pathogenic Vibrio spp. in co-culture experiments (in vitro). In vivo exclusion of Vibrio spp. in a Penaeus monodon postlarval rearing system was evaluated by treatment of the rearing water with biogranules of S. rubrolavendulae M56. The experiments proved that S. rubrolavendulae M56 biogranules could reduce the pathogenic Vibrio spp., while maintaining total heterotrophic bacterial count. Therefore, the actinomycete biogranules (M56) can be used as a promising alternative to antibiotics in the shrimp larval production system which is often affected by vibriosis.

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“…One of the factors that has been reported to affect the morphology of filamentous microorganisms is the inoculum size (Whitaker, 1992; (Glazebrook and Vining, 1992;Lawton et al, 1989;Smith and Calam, 1980;Tucker and Thomas, 1992;Tucker and Thomas, 1994). An inoculum with high concentration of viable spores tends to produce a disperse form of growth, whereas the use of an inoculum with low spore concentration normally results in pellet formation (Whitaker, 1992).…”

Section: Introductionmentioning

confidence: 99%

The inter-relationship between inoculum concentration, morphology, rheology and erythromycin productivity in submerged cultivation of Saccharopolyspora erythraea

Ghojavand

Bonakdarpour

Heydarian

et al. 2011

Braz. J. Chem. Eng.

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-Submerged cultivation of Saccharopolyspora erythraea, at different initial spore concentrations, was carried out to study the inter-relationship between inoculum concentration, morphology, rheology and erythromycin production. Pellet morphology was dominant in runs at 10 3 and 10 4 spore/ml initial spore concentrations, whereas there was a significant presence of clump morphology in runs at initial spore concentrations of 10 5 -10 7 spore/ml. The S. erythraea cultivation broths exhibited Newtonian rheology in runs at initial spore concentrations of 10 3 and 10 4 spore/ml, whereas at higher initial spore concentrations the rheological data could be fitted with the power law model. Runs in which clump morphology was predominant resulted in the highest erythromycin productivities. The findings of the present work suggest that the predominance of clump morphology, smaller sized clumps and, in the case of non-Newtonian S. erythraea cultivation broths, a decrease in viscosity enhance erythromycin production.

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Actinomycete morphology in shaken culture

Cited by 15 publications

References 9 publications

Bioaugmentation and Propane Biosparging for In Situ Biodegradation of 1,4-Dioxane

Bioaugmentation and Propane Biosparging for In Situ Biodegradation of 1,4-Dioxane

Exclusion ofVibriospp. by an antagonistic marine actinomyceteStreptomyces rubrolavendulaeM56

The inter-relationship between inoculum concentration, morphology, rheology and erythromycin productivity in submerged cultivation of Saccharopolyspora erythraea

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