Actin polymerization stabilizes α4β1 integrin anchors that mediate monocyte adhesion

Rullo, Jacob; Becker, Henry; Hyduk, Sharon J.; Wong, Janice; Digby, Geneviève C.; Arora, Pamma D.; Cano, Adrianet Puig; Hartwig, John H.; McCulloch, Christopher A.; Cybulsky, Myron I.

doi:10.1083/jcb.201107140

Cited by 39 publications

(30 citation statements)

References 64 publications

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“…The increased height and reduced area of cellular contact with VCAM-1 of cells treated with sorafenib suggest that these cells are experiencing greater shear stress distributed over a smaller area of anchorage, probably contributing to decreased adhesion in our laminar flow system. Cell adhesion has been shown to be dependent on the dynamics of the actin cytoskeleton and cell spreading, promoting adhesion strengthening and migration, in part by providing a more streamlined shape for the cell to reduce the shear stress imposed by laminar flow (6,31,32).…”

Section: Discussionmentioning

confidence: 99%

B-Raf Regulation of Integrin α4β1-mediated Resistance to Shear Stress through Changes in Cell Spreading and Cytoskeletal Association in T Cells

Brown

Khalili

Rodriguez-Cruz

et al. 2014

Journal of Biological Chemistry

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Section: Discussionmentioning

confidence: 99%

B-Raf Regulation of Integrin α4β1-mediated Resistance to Shear Stress through Changes in Cell Spreading and Cytoskeletal Association in T Cells

Brown

Khalili

Rodriguez-Cruz

et al. 2014

Journal of Biological Chemistry

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“…Such cell adhesion molecules have been identified and characterized to act in a sequential and interdependent manner. The molecular mechanism driving leukocyte integrin function during the multistep adhesion cascade is relatively well defined (4)(5)(6). In contrast, mechanisms underpinning the regulation of nonintegrin receptors are only beginning to emerge.…”

mentioning

confidence: 99%

L-selectin shedding is activated specifically within transmigrating pseudopods of monocytes to regulate cell polarity in vitro

Rzeniewicz

Newe

Gallardo

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

103

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L-selectin is a cell adhesion molecule that tethers free-flowing leukocytes from the blood to luminal vessel walls, facilitating the initial stages of their emigration from the circulation toward an extravascular inflammatory insult. Following shear-resistant adhesion to the vessel wall, L-selectin has frequently been reported to be rapidly cleaved from the plasma membrane (known as ectodomain shedding), with little knowledge of the timing or functional consequence of this event. Using advanced imaging techniques, we observe L-selectin shedding occurring exclusively as primary human monocytes actively engage in transendothelial migration (TEM). Moreover, the shedding was localized to transmigrating pseudopods within the subendothelial space. By capturing monocytes in midtransmigration, we could monitor the subcellular distribution of L-selectin and better understand how ectodomain shedding might contribute to TEM. Mechanistically, L-selectin loses association with calmodulin (CaM; a negative regulator of shedding) specifically within transmigrating pseudopods. In contrast, L-selectin/ CaM interaction remained intact in nontransmigrated regions of monocytes. We show phosphorylation of L-selectin at Ser 364 is critical for CaM dissociation, which is also restricted to the transmigrating pseudopod. Pharmacological or genetic inhibition of L-selectin shedding significantly increased pseudopodial extensions in transmigrating monocytes, which potentiated invasive behavior during TEM and prevented the establishment of front/back polarity for directional migration persistence once TEM was complete. We conclude that L-selectin shedding directly regulates polarity in transmigrated monocytes, which affirms an active role for this molecule in driving later stages of the multistep adhesion cascade.

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“…Cortical actin stabilizes integrin and cadherin adhesions and regulates intercellular tension. 43,44 Stress fibers in cytoplasm stabilize cell and generate traction force during migration and are ended with focal adhesion complexes. 6 ND, NG, and nGO nanoparticles decreased adhesion of cells to extracellular matrix showing similar effect to extracellular matrix with low mechanical rigidity.…”

mentioning

confidence: 99%

Diamond, graphite, and graphene oxide nanoparticles decrease migration and invasiveness in glioblastoma cell lines by impairing extracellular adhesion

Wierzbicki¹,

Jaworski²,

Kutwin³

et al. 2017

IJN

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The highly invasive nature of glioblastoma is one of the most significant problems regarding the treatment of this tumor. Diamond nanoparticles (ND), graphite nanoparticles (NG), and graphene oxide nanoplatelets (nGO) have been explored for their biomedical applications, especially for drug delivery. The objective of this research was to assess changes in the adhesion, migration, and invasiveness of two glioblastoma cell lines, U87 and U118, after ND, NG, and nGO treatment. All treatments affected the cell surface structure, adhesion-dependent EGFR/AKT/mTOR, and β-catenin signaling pathways, decreasing the migration and invasiveness of both glioblastoma cell lines. The examined nanoparticles did not show strong toxicity but effectively deregulated cell migration. ND was effectively taken up by cells, whereas nGO and NG strongly interacted with the cell surface. These results indicate that nanoparticles could be used in biomedical applications as a low toxicity active compound for glioblastoma treatment.

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Actin polymerization stabilizes α4β1 integrin anchors that mediate monocyte adhesion

Abstract: Rapid signaling and structural adaptations to the actin cytoskeleton enable leukocytes to stabilize α4 integrin–mediated adhesion and resist detachment from inflamed endothelium.

Cited by 39 publications

References 64 publications

B-Raf Regulation of Integrin α4β1-mediated Resistance to Shear Stress through Changes in Cell Spreading and Cytoskeletal Association in T Cells

B-Raf Regulation of Integrin α4β1-mediated Resistance to Shear Stress through Changes in Cell Spreading and Cytoskeletal Association in T Cells

L-selectin shedding is activated specifically within transmigrating pseudopods of monocytes to regulate cell polarity in vitro

Diamond, graphite, and graphene oxide nanoparticles decrease migration and invasiveness in glioblastoma cell lines by impairing extracellular adhesion

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